Morphological and Molecular Analysis and Flowering Time of T2 Generation Transgenic Rice cv. Nipponbare Carrying CONSTANS (CO) Gene

Authors

Firdha Meidi Irshanty, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Andi Salamah, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, IndonesiaFollow
Tri Joko Santoso, Molecular Biology Research Group, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetika Pertanian, Ministry of Agriculture, Bogor 16111, Indonesia

Abstract

Flowering is an important process that initiates plant productivity. Rice (Oryza sativa cv. Nipponbare) was genetically engineered by introducing a CONSTANS (CO) gene from Arabidopsis thaliana (AtCO) to improve rice productivity. The CO gene is a gene responsible for inducing early flowering. The aims of the experiment were to use morphological and molecular analysis to study the integration of the AtCO gene and its influence on agronomic traits in the T2 generation of Nipponbare transgenic AtCO rice. Morphological observations showed that agronomic traits tended to be better in the transgenic plants than in the wild type (controls), although the transgenic plants flowered later than the wild type. Polymerase Chain Reaction (PCR) analysis showed that 169 out of 227 transgenic rice plants showed hpt and CO gene integration.

References

[1] R.A. Fahliani, M. Khodambashi., S. Houshmand, A. Arzani., K. Sorkheh, Turk. J. Agric. For. 35 (2011) 481. [2] H. Gao, X. Zheng, G. Fei, J. Chen, M. Jin, Y. Ren, W. Wu, K. Zhou, P. Sheng, Feng Zhou1, L. Jiang, J. Wang, X. Zhang, X. Guo, J. Wang, Z. Cheng, C. Wu, H. Wang, J. Wan, PLoS Genet. 9 (2013) 1. [3] W.G. Hopkins, N.P.A Hüner, Introduction to Plant Physiology, 4th ed., John Wiley & Sons, Inc., Hoboken, 2009, p.418. [4] R.M. Amasino, S.D. Michaels, Plant Physiol. 154 (2010) 516. [5] R. Komiya, K. Shimamoto, Plant Biotechnol. 25 (2008) 279. [6] J. Putterill, Phil. Trans. R. Soc. Lond. B 356 (2001) 1762. [7] S.K. Yoo, K.S. Chung, J. Kim, J.H. Lee, S.M. Hong, S.J. Yoo, S.Y. Yoo, J.S. Lee, J.H. Ahn, Plant Physiol. 139 (2005) 770. [8] T. Izawa, Int. Rev. Cytol. 256 (2007) 195. [9] B. Thomas, J. Exp. Bot. 57 (2006) 3388. [10] P. Suarez-Lopez, K. Wheatley, F. Robson, Onouchi, F. Valverde & G. Coupland, Nature 410 (2001) 1116. [11] G. Coupland, Phil. Trans. R. Soc. Lond. B. 350 (1995) 30. [12] F.F. Tryani, Undergraduate Thesis, Department Biochemistry, Faculty of Mathematics and Sciences, Institut Pertanian Bogor, Indonesia, 2011 [In Indonesia]. [13] J. Finnegan, D. McElroy, In: M.R.L. Owen, J. Pen, Transgene Stability, Transgenic Plants: A Production System for Industrial and Pharmaceutical Proteins, John Wiley & Sons, New York, 1996, p.169. [14] I.H. Slamet-Loedin, S. Rahmawati, A. Estiati, S. Hugroho, E.S. Mulyaningsih, B. Saksono, A. Rachmat, T. Kuswara, Y. Sulistyowati, C. Pantouw, F. Rahman, Y. Andriani, S. Indrayani, Alvian, Lasimur, M. Sajam, A. Sutiawan, D. Soeharjo, D. Gozali, P. Zaenal, Maryanto, Sudiro, M. Napis, A.S. Surapermana, Biotechnology Research Project Technical Reports, Cibinong, Indonesia, 2001, p.42. [15] J.J. Doyle, J.L. Doyle, Phytochem. Bull. 19 (1987) 11 [16] Departemen Pertanian, Sekretariat Komisi Nasional Plasma Nutfah, Bogor, 2003, p.10,11,16,18,24. [in Indonesia] [17] A. Kohli, R.M. Twyman, R. Abranches, E. Wegel, E. Stoger, P. Christou, Plant Mol. Biol. 52 (2003) 247. [18] S. Yoshida, Fundamentals of Rice Crop Science, The International Rice Research Institute, Los Banõs, 1981, p.68. [19] J. Huang, X. Zhao, X. Weng, L. Wang, W. Xie, PLoS ONE 7 (2012) 1. [20] S. Hernando-Amado, V. González-Calle, P. Carbonero, C. Barrero-Sicilia, BMC Plant Biol. 12 (2012) 2. [21] P. Más, Int. J. Dev. Biol. 49 (2005) 496. [22] BMKG, Buletin Analisis Hujan Bulan Januari 2013 dan Perkiraan Hujan Bulan Maret, April, dan Mei, Stasiun Klimatologi Darmaga, Bogor, 2013. [in Indonesia] [23] R. Hayama, G. Coupland, Plant Physiol. 135 (2004) 682. [24] K. Miwa, M. Serikawa, S. Suzuki, T. Kondo, T. Oyama, Plant Cell Physiol.47 (2006) 610. [25] J.F. Martínez-García, A. Virgós-Soler, S. Prat, Proc. Nat. Acad. Sci. USA. 99 (2002) 15211. [26] A. Osugi, H. Itoh, K. Ikeda-Kawakatsu, M. Takano, T. Izawa, Plant Physiol. 157 (2001) 1128. [27] H. Tsuji, K. Taoka, K. Shimamoto, Curr. Opin. Plant Biol. 14 (2010) 4. [28] M. Yano, S. Kojima, Y. Takahashi, H. Lin, T. Sasaki, Plant Physiol. 127 (2001) 1425.

Recommended Citation

Irshanty, Firdha Meidi; Salamah, Andi; and Santoso, Tri Joko (2014) "Morphological and Molecular Analysis and Flowering Time of T2 Generation Transgenic Rice cv. Nipponbare Carrying CONSTANS (CO) Gene," Makara Journal of Science: Vol. 8: Iss. 1, Article 9.
DOI: 10.7454/mss.v18i1.3044
Available at: https://scholarhub.ui.ac.id/science/vol8/iss1/9