Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration

Authors

Indrajid Prayoga, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, Indonesia
Ani Mulyasuryani, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, IndonesiaFollow
Sasangka Prasetyawan, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, Indonesia

Abstract

Excessive diazinon residue in vegetables can endanger human health. Therefore, a simple, fast, and accurate method is needed to detect residue. A conductometric biosensor is a good choice because it also offers high selectivity and sensitivity. The principle of detection of the conductometric biosensor is based on enzymatic hydrolysis of diazinon into O,O diethyl phosphorothiate,2-isopropyl-6-methylpyrimidin-4-ol, and H+ catalyzed by organophosphate hydrolase (OPH). The optimum amount of organophosphate hydrolase added to the screen-printed carbon electrode (SPCE) modified with BSA-glutaraldehyde is 118.5 µg, while the optimum pH is 8.5. This biosensor has a response time of 30 sec, a linear dynamic range of 0 to 1 ppm, sensitivity of 42.21 µS/ppm, and limit of detection of 0.19 ppm.

References

[1] M. Pohanka, D. Jun, K. Kuca, Sensors 8 (2008) 5303. [2] M.N.V. Garcia, T. Mortram, Biosyst. Eng. 84 (2003) 1. [3] M. Pohanka, V. Adam, R. Kizek, Sensors 13 (2013) 11498. [4] N. Jaffrezic-Renault, S.V. Dzyadevych, Sensors 8 (2008) 2569. [5] M. Wyer, Metal Ion Promoted Hydrolysis of the Organophosphorus Pesticide, Diazinon, Queen’s University, Ontario, Canada, 2008. [6] B. Eggins, Chemical Sensors and Biosensors, John Wiley & Sons, Chichester, England, 2012, p.444. [7] B.T. Feyssa, Thesis, Faculty of Chemistry, University of Barcelona, Spain, 2010. [8] S.M. Naghib, M. Rabiee, E. Omidinia, P. Khoshkenar, D. Zeini, Int. J. Electrochem. Sci. 7 (2012) 120. [9] G.S. Nunes, G. Jeanty, J.L. Marty, Analytica Chimica Acta 523 (2004) 107. [10] N. Jaffrezic-Renault, Sensors 1 (2001) 60. [11] B. Krajewska, A. Olech, Polym. Gels Netw. 4 (1996) 33. [12] Y.A. Votchitseva, E.N. Efremenko, T.K. Aliev, S.D. Varfolomeyev, Biochemistry (Moscow), 71 (2006) 167. [13] A. Mulchandani, W. Chen, P. Mulchandani, J. Wang, K.R. Rogers, Biosens. Bioelectron. 16 (2001) 225. [14] W. Ningfeng, D. Minjie, S. Xiuyun, L. Guoyi, Y. Bin, F. Yunliu, Chinese Sci. Bull. 49 (2004) 268. [15] D. Rochu, N. Beaufet, F. Renault, N. Viguie, P. Masson, Biochimica et Biophysica Acta 1594 (2002) 207. [16] D.A. Kraut, P.A. Sigala, B. Pybus, C.W. Liu, D. Ringe, G.A. Petsko, D. Herschlag, Plos Biology 4 (2006) 501. [17] J.N. Miller, J.C. Miller, Statistics and Chemometrics for Analytical Chemistry, Pearson Education, Harlow, England, 2010, p.296.

Recommended Citation

Prayoga, Indrajid; Mulyasuryani, Ani; and Prasetyawan, Sasangka (2014) "Construction and Characterization of Conductometric Biosensor for Determination of the Diazinon Concentration," Makara Journal of Science: Vol. 8: Iss. 1, Article 12.
DOI: 10.7454/mss.v18i1.3051
Available at: https://scholarhub.ui.ac.id/science/vol8/iss1/12