AKTIVITAS LIPOLITIK Rhizopus microsporus var. rhizopodiformis ISOLAT UICC No. 6

Authors

Iman Iman, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, IndonesiaFollow
Sitaresmi Sitaresmi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, Indonesia
Doni Prayudi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, Indonesia
Kudin Kudin, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, Indonesia

Abstract

The lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. A study was carried out to examine the effect of peptone concentration (2,5% or 5%), inoculum concentration (0,1%, 0,5% or 1%) and incubation period (0 – 96 hours, interval 12 hours) on the lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. Fermentation was done using the basal medium from Samad and the lipolytic activity on olive oil substrate was measured employing titration method with 0,05M NaOH. Lipolytic activity is expressed as unit/ml and one unit is defined as 1 mmol fatty acid liberated per minute. Results show that optimum lipolytic activity was obtained from 1 % inoculum, 5% peptone after 72 hours incubation period

References

[1] M.F. Chaplin, C. Bucke, Enzyme technology, Cambridge University Press, Cambridge, 1990. [2] I. Gandjar, Indonesian Food and Nutrition Progress 2 (1995) 51. [3] W. Crueger, A. Crueger, Biotechnology: A Textbook of Industrial Microbiology, Science Tech. Inc., Madison, 1984. [4] M.A. Rifai, UNESCO Regional Workshop on Culture Collection of microorganisms in Southeast Asia, PAU Pangan & Gizi UGM, Yogyakarta, 1995. [5] I. Santoso, UNESCO Regional Workshop on Culture Collection of microorganisms in Southeast Asia, PAU Pangan dan Gizi UGM, Yogyakarta, 1995, p.1. [6] M.Y.A. Samad, A.B. Salleh, C.N.A. Razak, K. Ampon, W. M. Z. Yunus, M. Basri, World J. of Microbiol & Biotech. 6 (1990) 390. [7] P.J. Fellow, Food Processing Technology, Ellis Horwood, New York (1990), p. 505. [8] E. Nahas. J. Gen. Microbiol. 134 (1988) 227. [9] G. Lazar, F.R. Schroder. In : G. Winkerman (Ed.), Microbial Degradation of Natural Products, VCH, Weinheim (1992) 267. [10] Y. Gao, C. Breuil. J. of Microbiol. & Biotech. 11 (1990) 638. [11] C.L. Cooney, In : H.J. Rehm, & G. Reed (Eds.). Biotechnology 1, Verlag Chemie, Wienheim (1981) p. 73

Recommended Citation

Iman, Iman; Sitaresmi, Sitaresmi; Prayudi, Doni; and Kudin, Kudin (2002) "AKTIVITAS LIPOLITIK Rhizopus microsporus var. rhizopodiformis ISOLAT UICC No. 6," Makara Journal of Science: Vol. 6: Iss. 3, Article 9.
Available at: https://scholarhub.ui.ac.id/science/vol6/iss3/9