Optimization of Laccase Adsorption-Desorption Behaviors on Multi-Walled Carbon Nanotubes for Enzymatic Biocathodes

Authors

Alex Lukmanto Suherman, 1. Faculty of Medicine and Pharmacy, University of Grenoble Alpes, Grenoble 38400, France. 2. Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University South Parks Road, Oxford OX1 3QZ, United Kingdom. 3. Laboratoire TIMC-IMAG équipe Systèmes Nanobiotechnologiques, Biomimetiques et Biotechnologies, UMR 5525 CNRS, Grenoble F-38041, FranceFollow
Abdelkader Zebda, Laboratoire TIMC-IMAG équipe Systèmes Nanobiotechnologiques, Biomimetiques et Biotechnologies, UMR 5525 CNRS, Grenoble F-38041, France
Donald K. Martin, 1. Faculty of Medicine and Pharmacy, University of Grenoble Alpes, Grenoble 38400, France. 3. Laboratoire TIMC-IMAG équipe Systèmes Nanobiotechnologiques, Biomimetiques et Biotechnologies, UMR 5525 CNRS, Grenoble F-38041, France

Abstract

Laccase adsorption-desorption behaviors on the surface of multi-walled carbon nanotubes (MWCNTs) were investi-gated using spectrophotometry and voltammetry. The optimum condition for laccase adsorption is 5.0 mg/mL of laccase in 0.01 M phosphate-buffered saline (PBS) at pH 5.0. Laccase adsorption is a reversible phenomenon that is dependent upon the nature of MWCNTs and the concentration of ionic strength in the laccase solution. Chitosan was functiona-lized as a nanoporous reservoir to minimize laccase desorption. Chitosan was found to protect approximately 97.2% of the adsorbed laccase from MWCNTs during the first six hours of observation. The three-dimensional (3D) biocathode, MWCNTs-laccase-chitosan with a 0.2 cm2 geometric area, was shown to have a stable open circuit potential (OCP) of 0.55 V, a current density of 0.33 mA cm-2 at 0.2 V vs. saturated calomel electrode (SCE), and a stable current for 20 hours of successive measurements. This report provides a new insight into the study of a high-performance laccase-based biocathode via optimization of adsorption and minimization of desorption phenomena.

Recommended Citation

Suherman, Alex Lukmanto; Zebda, Abdelkader; and Martin, Donald K. (2018) "Optimization of Laccase Adsorption-Desorption Behaviors on Multi-Walled Carbon Nanotubes for Enzymatic Biocathodes," Makara Journal of Science: Vol. 22: Iss. 1, Article 7.
DOI: 10.7454/mss.v22i1.8619
Available at: https://scholarhub.ui.ac.id/science/vol22/iss1/7