Electrochemical Detection of Neuraminidase Based on Zanamivir Inhibition Reaction at Platinum and Platinum-Modified Boron-Doped Diamond Electrodes

Authors

Tribidasari Anggraningrum Ivandini, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, IndonesiaFollow
Jenny Ariani, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Prastika Krisma Jiwanti, Department of Chemistry, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan
Jarnuzi Gunlazuardi, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Endang Saepudin, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Yasuaki Einaga, 2. Department of Chemistry, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan. 3. JSTACCEL, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.

Abstract

Neuraminidase sensors have been developed using platinum (Pt) and platinum-modified boron-doped diamond (Pt-BDD) electrodes based on the difference of electrochemical responses of zanamivir in the absence and in presence of neuraminidase. Cyclic voltammetry of zanamivir showed that the presence of zanamivir decreases the oxidation-reduction peak currents of Pt. The responses were found to be linear in the zanamivir concentration range of 7.5-150 μM. Comparison between Pt-BDD and Pt bulk electrodes showed that Pt-BDD offers approximately two times higher sensitivity of zanamivir responses than Pt bulk. Further comparison to gold-based electrodes showed that Pt-based electrodes provided about one order higher sensitivity of zanamivir responses. In the presence of neuraminidase, the oxidation-reduction currents of Pt again increase. Observation on the oxidation currents in the system with 2 x 10-5 M zanamivir showed that the responses were linear in the neuraminidase concentration range of 5-20 mU. Good reproducibility with an RSD < 3 (n = 10) was achieved even when the measurements were performed in a mucin matrix,suggesting that Pt-based electrodes are suitable to be applied for the detection method of neuraminidase.

Recommended Citation

Ivandini, Tribidasari Anggraningrum; Ariani, Jenny; Jiwanti, Prastika Krisma; Gunlazuardi, Jarnuzi; Saepudin, Endang; and Einaga, Yasuaki (2017) "Electrochemical Detection of Neuraminidase Based on Zanamivir Inhibition Reaction at Platinum and Platinum-Modified Boron-Doped Diamond Electrodes," Makara Journal of Science: Vol. 21: Iss. 1, Article 6.
DOI: 10.7454/mss.v21i1.7535
Available at: https://scholarhub.ui.ac.id/science/vol21/iss1/6