Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine

Authors

Wiyogo P. Wicaksono, Department of Chemistry, Faculty of Mathematics and Science, Universitas Indonesia, Depok 16424, Indonesia
Tribidasari A. Ivandini, Department of Chemistry, Faculty of Mathematics and Science, Universitas Indonesia, Depok 16424, IndonesiaFollow
Endang Saepudin, Department of Chemistry, Faculty of Mathematics and Science, Universitas Indonesia, Depok 16424, Indonesia
Yasuaki Einaga, Department of Chemistry, Faculty of Science and Technology, Keio University 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan . JST-CREST, 3-14-1 Hiyoshi, Yokohama 2238522, Japan

Abstract

An immunochromatographic strip test based on the complex reaction of antigen-antibody (melamine-antimelamine) was developed for quantitative detection of melamine. Gold nanoparticles (AuNP) were used to form AuNP-labeled antibody, which then acted as a biosensor. Melamine quantification was performed by the determination of AuNP using anodic stripping voltammetry technique with a boron-doped diamond as the working electrode. With sample volume of 100 µL and immunoreaction time of 7 min, the developed immunochromatographic strip test produced a linear calibration curve for melamine concentration range of 0–0.6 mg/L, with detection limit of 0.1 mg/L and RSD of ~5%. Furthermore, negative results were obtained for samples containing cyanuric acid and urea, indicating that the developed immunochromatographic strip test has potential for selective and quantitative detection of melamine.

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Recommended Citation

Wicaksono, Wiyogo P.; Ivandini, Tribidasari A.; Saepudin, Endang; and Einaga, Yasuaki (2014) "Development of Immunochromatographic Strip Tests for Selective and Quantitative Detection of Melamine," Makara Journal of Science: Vol. 18: Iss. 3, Article 6.
DOI: 10.7454/mss.v18i3.3722
Available at: https://scholarhub.ui.ac.id/science/vol18/iss3/6