•  
  •  
 

Abstract

Identification of the genus Cercospora is still complicated due to the host preferences often being used as the main criteria to propose a new name. We determined the relationship between host plants and multilocus sequence variations (ITS rDNA including 5.8S rDNA, elongation factor 1-α, and calmodulin) in Cercospora spp. to investigate the host specificity. We used 53 strains of Cercospora spp. infecting 12 plant families for phylogenetic analysis. The sequences of 23 strains of Cercospora spp. infecting the plant families of Asteraceae, Cucurbitaceae, and Solanaceae were determined in this study. The sequences of 30 strains of Cercospora spp. infecting the plant families of Fabaceae, Amaranthaceae, Apiaceae, Plumbaginaceae, Malvaceae, Cistaceae, Plantaginaceae, Lamiaceae, and Poaceae were obtained from GenBank. The molecular phylogenetic analysis revealed that the majority of Cercospora species lack host specificity, and only C. zinniicola, C. zeina, C. zeae-maydis, C. cocciniae, and C. mikaniicola were found to be host-specific. Closely related species of Cercospora could not be distinguished using molecular analyses of ITS, EF, and CAL gene regions. The topology of the phylogenetic tree based on the CAL gene showed a better topology and Cercospora species separation than the trees developed based on the ITS rDNA region or the EF gene.

References

[1] P.W. Crous, U. Braun, Mycosphaerella and its Anamorphs, 1. Names published in Cercospora and Passalora. CBS Biodiversity Series 1, Utrecht, Netherland, 2003, p.571. [2] S. Simons, Management Strategies for Maize Grey Leaf Spot (Cercospora zeae-maydis) in Kenya and Zimbabwe, http://www.dfid.gov.uk/r4d/SearchResearchDataba se.asp?OutPutId=180879, 2003. [3] J. Weiland, G. Koch, Molecular Plant Pathology, 5 (2004) 157. [4] R.T. Lartey, T.C. Caesar-TonThat, A.J. Caesar, W.L. Shelver, N.I. Sol, J.W. Bergman, Plant Dis. 89 (2005) 797. [5] S.C. Nelson, Plant Dis. 41 (2008) 1. [6] M.E. Daub, K.R. Chung, In: K. Esser, H.B. Deising (eds.), Plant relationships. 2nd ed. The Mycota V: A Comprehensive Treatise on Fungi as a Experimental Systems for Basic and Applied Research, Springer-Verlag Berlin Heidelberg, Germany, 2009, p.397. [7] C. To-anun, J. Nguenhom, J. Meeboon, I. Hidayat, Mycol. Prog. 8 (2009) 115. [8] C. To-anun, I. Hidayat, J. Meeboon, J. Agric. Technol. 6 (2010) 331. [9] P.W. Crous, A. Aptroot, J.-C. Kang, U. Braun, M.J. Wingfield, Stud. Mycol. 45 (2000) 107. [10] P.W. Crous, G.J.M. Verkley, J.Z. Groenewald, R.A. Samson, Fungal Diversity, CBS-KNAW Fungal Biodiversity Centre, Netherland, 2009, p.269. [11] P.W. Crous, J.Z. Groenewald, K. Pongpanich, W. Himawan, M. Arzanlaou, M.J. Wingfield, Stud. Mycol. 50 (2004) 457. [12] V. Ayala-Escobar, M.J. Yanez-Morales, U. Braun, J.Z. Groenewald, P.W. Crous, Mycotaxon 93 (2005) 115. [13] M. Groenewald, J.Z. Groenewald, P.W. Crous, Phytophatology. 95 (2005) 951. [14] M. Groenewald, J.Z. Groenewald, U. Braun, P.W. Crous, Mycologia. 98 (2006) 275. [15] P.W. Crous, J.Z. Groenewald, M. Groenewald, P. Caldwell, U. Braun, T.C. Harrington, Stud. Mycol. 55 (2006) 189. [16] C. Chupp, A Monograph of the Fungus Genus Cercospora, Ithaca, New York, 1954, p.667. [17] C. To-Anun, I. Hidayat, J. Meeboon, Plant Pathology & Quarantine. 1 (2011) 11. [18] T.J. White, T. Bruns, J. Taylor, In: M.A. Innis, D.H. Gelfand, J.J. Sninsky, T. White (Eds.), A Guide to Molecular Methods and Applications, Academic Press, New York, 1990, p.315. [19] I. Carbone, L.M. Kohn, L.M, Mycologia. 91 (1999) 553 [20] K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28 (2011) 2731. [21] N. Saitou, M. Nei, Mol. Biol. Evol. 4 (1987) 406. [22] J. Felsenstein, Evol. 39 (1985) 783. [23] R.D.M. Page, Comput. Appl. Biosci. 12 (1996) 357. [24] J.S. Farris, M. Kallersjo, A.G. Kluge, C. Bult, Cladistic 10 (1995) 315. [25] S.B. Goodwin, L.D. Dunkle, V.L. Zismann, Phytopathology 91 (2001) 648. [26] J. Wang, M. Levy, L.D. Dunkle, Phytopathology 88 (1998) 1269. [27] D.M. Geiser, M.M. Jimenez-Gasco, S. Kang, I. Makalowska, N. Veeraraghavan, T.J. Ward, N. Zhang, G.A. Kuldau & K. O’Donnell, Eur. J. Plant Pathol. 110 (2004) 473.

Download

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.