ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P

Authors

Ratna Yuniati, Research Center for Bioresources and Biotechnology, Institut Pertanian Bogor, Bogor 16680, Indonesia. Department of Biology, Institut Pertanian Bogor, Bogor 16680, Indonesia
Utut Widyastuti, Research Center for Bioresources and Biotechnology, Institut Pertanian Bogor, Bogor 16680, Indonesia. Department of Biology, Institut Pertanian Bogor, Bogor 16680, Indonesia
Didy Sopandie, Department of Agronomy and Horticulture, Institut Pertanian Bogor, Bogor 16680, Indonesia
Akiho Yokota, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
Kinya Akashi, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
Suharsono Suharsono, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, JapanFollow

Abstract

Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotide sequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis.

References

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Recommended Citation

Yuniati, Ratna; Widyastuti, Utut; Sopandie, Didy; Yokota, Akiho; Akashi, Kinya; and Suharsono, Suharsono (2011) "ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P," Makara Journal of Science: Vol. 15: Iss. 2, Article 27.
Available at: https://scholarhub.ui.ac.id/science/vol15/iss2/27