AKTIVITAS LIPOLITIK Rhizopus microsporus var. rhizopodiformis ISOLAT UICC No. 6

Authors

Iman Iman, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, IndonesiaFollow
Sitaresmi Sitaresmi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, Indonesia
Doni Prayudi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, Indonesia
Kudin Kudin, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Indonesia, Depok 16424, Indonesia

Abstract

The lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. A study was carried out to examine the effect of peptone concentration (2,5% or 5%), inoculum concentration (0,1%, 0,5% or 1%) and incubation period (0 – 96 hours, interval 12 hours) on the lipolytic activity of Rhizopus microsporus var rhizopodiformis isolat UICC No. 6. Fermentation was done using the basal medium from Samad and the lipolytic activity on olive oil substrate was measured employing titration method with 0,05M NaOH. Lipolytic activity is expressed as unit/ml and one unit is defined as 1 mmol fatty acid liberated per minute. Results show that optimum lipolytic activity was obtained from 1 % inoculum, 5% peptone after 72 hours incubation period

References

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Recommended Citation

Iman, Iman; Sitaresmi, Sitaresmi; Prayudi, Doni; and Kudin, Kudin (2002) "AKTIVITAS LIPOLITIK Rhizopus microsporus var. rhizopodiformis ISOLAT UICC No. 6," Makara Journal of Science: Vol. 6: Iss. 3, Article 1.
Available at: https://scholarhub.ui.ac.id/science/vol6/iss3/1