Porous Calcium Carbonate-Poly-2-Acrylamido-2-Methylpropanesulfonic Acid Microspheres Embedded with Silver-Based Nanoparticles as Potential Antibacterial Carriers for Bone Infections

Authors

Novi Dwi Widya Rini, Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Bandung 40132, Indonesia
Adinda Asmoro, Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Bandung 40132, Indonesia
Systi Adi Rachmawati, Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Bandung 40132, Indonesia
Husaini Ardy, Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Bandung 40132, Indonesia
Akfiny Hasdi Aimon, Department of Physics, Faculty of Mathematical and Natural Sciences, Institut Teknologi Bandung, Bandung 40132, Indonesia
Akihiro Kishimura, Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 819-0395, Japan
Yoshiki Katayama, Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 819-0395, Japan
arie wibowo, Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Bandung 40132, IndonesiaFollow

Abstract

Implant-associated infections (IAIs) are a significant complication following orthopedic surgeries and are mainly caused by Staphylococcus aureus. In this study, microspheres based on hybrid calcium carbonate–poly-2-acrylamido-2-methylpropane sulfonic acid (CaCO₃-PAMPS) embedded with silver-based nanoparticles (AgNPs) were developed as potential antibacterial drug carriers for IAIs. AgNPs were prepared via a green synthesis approach, which employed Indonesian wild honey as a reducing and capping agent. PAMPS concentrations (0.025%, 0.050%, and 0.075% w/v) and silver ion (Ag⁺) concentrations in solution (0%, 2.5%, and 4% w/v) were varied. Scanning electron microscopy images showed that samples with 0.050% w/v PAMPS and 2.5% w/v Ag⁺ exhibited remarkable stability against recrystallization and aggregation. The sample exhibited more homogeneous, free-standing vaterite particles. Moreover, the particles obtained displayed a porous structure, promising for loading active ingredients. In addition, the X-ray diffraction results confirmed that the Ag-based particles embedded were not only AgNPs but were also predominantly composed of AgCl. AgNPs and AgCl in this sample demonstrated antibacterial activity against Staphylococcus aureus (zone of inhibition of 7.2 ± 0.6 mm). The development of stable, homogeneous microspheres with antibacterial properties and drug-loading potential offers a promising solution for the prevention and treatment of IAIs, addressing a critical challenge in orthopedic surgery.

Recommended Citation

Rini, Novi Dwi Widya; Asmoro, Adinda; Rachmawati, Systi Adi; Ardy, Husaini; Aimon, Akfiny Hasdi; Kishimura, Akihiro; Katayama, Yoshiki; and wibowo, arie (2025) "Porous Calcium Carbonate-Poly-2-Acrylamido-2-Methylpropanesulfonic Acid Microspheres Embedded with Silver-Based Nanoparticles as Potential Antibacterial Carriers for Bone Infections," Makara Journal of Science: Vol. 29: Iss. 2, Article 3.
DOI: 10.7454/mss.v29i2.2424
Available at: https://scholarhub.ui.ac.id/science/vol29/iss2/3