Mutation of Aspergillus sp. using Ultraviolet Light and Nitrous Acid for Amylase Production from Banana Peels

Authors

Cyprian Erumiseli Oshoma, Department of Microbiology, University of Benin, Benin City 300213, NigeriaFollow
Johnson Oche Akor, Department of Microbiology, University of Benin, Benin City 300213, Nigeria
Beckley Ikhajiagbe, Department of Plant Biology and Biotechnology, University of Benin, Benin City 300213, Nigeria
Marcel James Ikenebomeh, Department of Microbiology, University of Benin, Benin City 300213, Nigeria

Abstract

Strain improvement through mutagenesis is important in the commercial development of microbial fermentation. This study aimed to produce amylase from banana (Musa sapientum) peels by using mutant Aspergillus sp. strains obtained via ultraviolet (UV) radiation and nitrous acid-based mutagenesis. Results revealed that banana peels pretreated with 0.8 N HCl had a biomass yield of 3.02 g/L and amylase activity of 2.81 U/L. Of the UV mutants, strain AUV1 had the highest biomass and amylase activity of 4.50 ± 0.21 g/L and 3.46 ± 0.14 U/L, respectively. Subsequently, HNO₂ mutants showed that strain ANA6 had 30.64% and 60.85% higher amylase activity than AUV1 and the wild strain, respectively. This study showed that banana peels can be utilized for amylase production. In addition, UV and HNO₂ are effective mutagens for Aspergillus sp. strain improvement to enhance amylase productivity.

Recommended Citation

Oshoma, Cyprian Erumiseli; Akor, Johnson Oche; Ikhajiagbe, Beckley; and Ikenebomeh, Marcel James (2022) "Mutation of Aspergillus sp. using Ultraviolet Light and Nitrous Acid for Amylase Production from Banana Peels," Makara Journal of Science: Vol. 26: Iss. 3, Article 7.
DOI: 10.7454/mss.v26i3.1357
Available at: https://scholarhub.ui.ac.id/science/vol26/iss3/7