Hemoglobin-Modified Core–Shell Fe3O4@Au Nanostructures for the Electrochemical Detection of Acrylamide

Authors

Endang Saepudin, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Tri Yuliani, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Mochammad Arfin Fardiansyah Nasution, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Munawar Khalil, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Jong Wook Hong, Department of Chemistry, University of Ulsan, Ulsan 44776, South Korea
Tribidasari Anggraningrum Ivandini, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, IndonesiaFollow

Abstract

In this study, electrochemical detection of acrylamide using hemoglobin (Hb)-modified core–shell Fe₃O₄@Au nanostructures was conducted. Fe₃O₄ nanoparticles (~4.9 nm) and core–shell Fe₃O₄@Au (5.0–6.4 nm) nanostructures were successfully synthesized by the thermal decomposition method. Electrochemical investigation revealed that the optimum amount of Hb of 2 mg/mL could be immobilized in 0.1 M acetate buffer solution (pH = 6). Moreover, the detection of acrylamide using Fe₃O₄@Au/Hb was evaluated by the cyclic voltammetry technique. A linear calibration curve (R² = 0.98) in the concentration range of 0.1 to 1.0 µM could be achieved with an estimated limit of detection, limit of quantification, and sensitivity of 0.136 µM, 0.453 µM, and 0.4411 µA/µM, respectively. Furthermore, the developed biosensor exhibited high selectivity in the presence of ascorbic acid, melamine, and caffeine. The developed biosensor was applied to the detection of acrylamide in coffee samples and validated using the standard high-performance liquid chromatography (HPLC) method. The concentration of acrylamide in coffee samples was determined to be 37.450 and 35.377 ppm using electrochemical measurement and HPLC, respectively.

Recommended Citation

Saepudin, Endang; Yuliani, Tri; Nasution, Mochammad Arfin Fardiansyah; Khalil, Munawar; Hong, Jong Wook; and Ivandini, Tribidasari Anggraningrum (2021) "Hemoglobin-Modified Core–Shell Fe3O4@Au Nanostructures for the Electrochemical Detection of Acrylamide," Makara Journal of Science: Vol. 25: Iss. 3, Article 1.
DOI: 10.7454/mss.v25i3.1232
Available at: https://scholarhub.ui.ac.id/science/vol25/iss3/1