Abstract
Phylogentic Relationship among Limnonectes (Ranidae: Amphibia) found in West Sumatra with Other Species from South East Asia based on the based on the 16S rRNA Gen. The objective of this research was to study the phylogenetic relationship among Limnonectes species found in West Sumatra and with other species from South East Asia based on the partial DNA sequences16S rRNA sequences. DNA sequences were aligned using ClustalX version 1.64b, and the phylogenetic relationship within samples were analyzed using PHYLIP version 3.5c program. The alignment showed that, from 805 sites, there are 250 parsimony informative polymorphism sites. The phylogenetic tree showed that all of the Limnonectes spesies were divided in two clusters, the L. blythii complex and L. kuhlii complex. L. kuhlii and L. sp1 clustered into L. kuhlii complex and L. shomponerum and L. macrodon were clustered to L. blythii complex. This result showed that L. kuhlii and L. blythii are species complexes that are actually constituted of several species.
References
[1] S.B. Emerson, D. Berringan, Herpetologica, 49 (1993) 22. [2] S.B. Emerson, R. Ward, Zool. J. Linn. Soc. (1998) 537. [3] S.B. Emerson, R.F. Inger, D.T. Iskandar, Mol. Phyl. Evol. 16/1 (2000) 131. [4] S.P. Fuller, Baverstock, D. King, Mol. Phyl. Evol. 9 (1998) 294. [5] J. Sambrook, E.F. Frissch, T. Maniatis, Molecular Cloning A Laboratoty Manual, vol. 3, Cold Spring Harbor Laboratory Press, New York, 2006, p.215. [6] J. Felsenstein, Evolution 39 (1985) 783. [7] M. Kuramoto, Bull. Fukuoka Univ. Ed. 33 (1983) 83. [8] D.T. Iskandar, Amfibi Jawa dan Bali, Puslitbang Biologi-LIPI, 1998. [9] R.A.F.P. Sari, Skripsi Sarjana, Jurusan Biologi, FMIPA, ITB, Bandung, 1996. [10] H.K. Voris, Maps Journal Biogeography 27 (2000) 1153. [11] R. Hall, In: R. Hall and J. Holloway, Biogeography and Geological Evolution of SE Asia, Backhuys, Leiden, 1998, p.99. [12] J.C. Avise, Molecular Markers, Natural History and Evolution, New York: Chapman and Hall, 1994, p.100. [13] J. Kosuch, M. Veith, uni-halle.de download//04 abstract. Pdf. 2002. [14] R.F Inger, H.K. Voris, J. of Biogeography 28 (2001) 863. [15] D.T. Iskandar, E. Colijn, Treubia 31(Supplement), 2001, p.252.
Recommended Citation
Tjong, Djong Hon; Iskandar, Djoko T.; and Gusman, David
(2010)
"HUBUNGAN FILOGENETIK SPESIES LIMNONECTES (RANIDAE: AMPHIBIA) ASAL SUMATERA BARAT DAN ASAL ASIA TENGGARA BERDASARKAN GEN 16S RIBOSOMAL RNA,"
Makara Journal of Science: Vol. 14:
Iss.
1, Article 20.
Available at:
https://scholarhub.ui.ac.id/science/vol14/iss1/20
