PENGARUH PENAMBAHAN URASIL DALAM MEDIA FERMENTASI TERHADAP HASIL β-GLUKAN DARI DUA GALUR AGROBACTERIUM

Authors

Kusmiati Kusmiati, Pusat Penelitian Bioteknologi, LIPI, Cibinong, Bogor 16911, IndonesiaFollow
Swasono R. Tamat, Pusat Radioisotop dan Radiofarmaka, BATAN, Kawasan Puspiptek, Serpong, Banten 15310, Indonesia. Fakultas Farmasi, Universitas Pancasila, Jakarta 12640, Indonesia
Sukma Nuswantara, Pusat Penelitian Bioteknologi, LIPI, Cibinong, Bogor 16911, Indonesia
Salmah Muhamad, Fakultas Farmasi, Universitas Pancasila, Jakarta 12640, Indonesia

Abstract

Influence of Uracil in Fermentation Media on β-Glucan Production by Agrobacterium Radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1. Optimum β-glucan production can be achieved by an optimum condition in the fermentation media. Uracil, as a precursor of UDP-glucose, may act as a glucose donor in the formation of polysaccharides such as β-glucan. It is expected that addition of certain quantity of uracil into the fermentation media in a suitable growth phase of Agrobacterium radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1, will significantly increase the β-glucan production. In this investigation, 0.025%; 0.05% or 0.1% of uracil were added into the fermentation media during the logarithmic phase (24 hour) or stationary phase (46 hour) of growth. The β-glucan product was evaluated from the β-glucan (crude) dry-weight and from the β-glucan content. Beta-glucan content was determined as glucose by the Hisamatsu-AOAC and HPLC methods. The highest β-glucan (crude) dry-weight produced by the A. 1.5 was in a medium containg 0.025% uracil (24 hour), whilst by the A. Bro 1.2.1 was in a medium containg 0.1% uracil (46 hour), both higher than control. The highest β-glucan content produced by the A. 1.5 (27.03%) was in a medium containg 0.025% uracil (46 hour), while control produced only 23.28%. The highest β-glucan content produced by the Bro 1.2.1 (29.34%) was in a medium containg 0.025% uracil (24 hour), while control produced only 28.75%. Two-way anova analysis showed that there were no significant influence difference (α = 0,05) from various concentration of uracil in either growth phases, to the yield of β-glucan (crude) dry-weight nor to the β-glucan equivalent glucose content.

References

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Recommended Citation

Kusmiati, Kusmiati; Tamat, Swasono R.; Nuswantara, Sukma; and Muhamad, Salmah (2007) "PENGARUH PENAMBAHAN URASIL DALAM MEDIA FERMENTASI TERHADAP HASIL β-GLUKAN DARI DUA GALUR AGROBACTERIUM," Makara Journal of Science: Vol. 11: Iss. 2, Article 17.
Available at: https://scholarhub.ui.ac.id/science/vol11/iss2/17