The Biocompatibility of a Ginger-Containing Herbal Toothpaste on Developing Zebrafish Embryos

Authors

Simge Meseli, Department of Restorative Dentistry, Faculty of Dentistry, Marmara University, Istanbul, TurkeyFollow
Unsal Veli Ustundag, Department of Biochemistry, Faculty of Medicine, Istanbul Medipol University, Istanbul, TurkeyFollow
Perihan Seda Ates, Department of Biochemistry, Faculty of Pharmacy, Saglik ve Teknoloji University, Istanbul, TurkeyFollow
Ismail Unal, Department of Biochemistry, Faculty of Dentistry, Marmara University, Istanbul, TurkeyFollow
Ebru Isik Alturfan, Department of Biochemistry, Faculty of Dentistry, Marmara University, Istanbul, TurkeyFollow
Dilek Tağtekin, Department of Restorative Dentistry, Faculty of Dentistry, Marmara University, Istanbul, TurkeyFollow
Funda Yanikoglu, Department of Restorative Dentistry, Faculty of Dentistry, Istanbul Kent University, Istanbul, TurkeyFollow

Abstract

The biocompatibility of toothpaste in an oral cavity should be approved by clinical trials. Nowadays, herbal toothpaste is increasing in popularity due to its natural ingredients. Being genetically similar to humans, zebrafish are used in potential toxicity testing. The zebrafish embryotoxicity test is a fast and straightforward method to study chemical toxicity during embryogenesis.

Objective: This study aimed to evaluate if there was any biocompatibility of the toothpaste on zebrafish embryos.

Methods: Adult AB strain zebrafish were used according to Institutional Animal Care and Use Committee protocols. Normally dividing, spherical embryos were exposed to herbal toothpaste with ginger (Gumgumix, Turkey) (50 mg/L) and conventional toothpaste (Signal, Expert Protection, Bulgaria) (50 mg/L) in well plates containing 20 embryos, having four replicates. Developmental effects, mortality, and hatching rates were evaluated for 72h.

Results: Zebrafish embryos exposed to conventional toothpaste had a higher mortality rate than those exposed to herbal toothpaste; they hatched later and delayed in development. There was no difference between herbal toothpaste and the control group regarding mortality and hatching rates (p > 0.005).

Conclusion: The herbal toothpaste showed higher biocompatibility on zebrafish embryos compared to the conventional toothpaste under the condition of this study.

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Recommended Citation

Meseli, S., Ustundag, U. V., Ates, P. S., Unal, I., Alturfan, E. I., Tağtekin, D., & Yanikoglu, F. The Biocompatibility of a Ginger-Containing Herbal Toothpaste on Developing Zebrafish Embryos. J Dent Indones. 2023;30(2): 115-120