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Abstract

Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was cultured in Sabouraud dextrose broth at 37°C for 48 hours. A total of 24 single-rooted premolar teeth were standardized and inoculated with C. albicans before irrigation with 50 μg/mL reuterin as a single, independent variable (Indonesian strain), 50 μg/mL reuterin Prodentis (a strain combination of L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289), 2.5% sodium hypochlorite as positive control, and saline as negative control. A real-time quantitative polymerase chain reaction (RT-qPCR) assay was used to detect the expression of BCR1, ACE2, EFG1, and TEC1 in C. albicans root canal biofilms. Results: Reuterin significantly reduced the expression of BCR1 and ACE2 genes, which play a role in C. albicans biofilm formation, at the biofilm maturation stage (P < 0.05). Reuterin also affected the expression of the EFG1 and TEC1 genes, although the effect was not significant. Conclusion: A reuterin isolate of L. reuteri exhibits antibiofilm activity against the expression of C. albicans genes involved in biofilm formation. Reuterin has potential as an irrigation agent in the treatment of root canals. Further research is needed to shed light on the effectiveness of reuterin against the expression of genes that play important roles in the formation of C. albicans biofilms.

References

  1. GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017;390(10100):1211-59.
  2. Shuler CF. Inherited risks for susceptibility to dental caries. J Dent Educ. 2001;65(10):1038-45.
  3. Sampaio-Maia B, Caldas IM, Pereira ML, Pérez- Mongiovi D, Araujo R. The oral microbiome in health and its implication in oral and systemic diseases. Adv Appl Microbiol. 2016;97:171-210.
  4. Peciuliene V, Maneliene R, Balcikonyte E, Drukteinis S. Microorganisms in root canal infections : a review. Balt Dent Maxillofac J. 2008;10(1):4–9.
  5. Siqueira JF Jr, Sen BH. Fungi in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97(5):632-41.
  6. Thosar N, Chandak M, Bhat M, Basak S. Antifungal effect of zinc oxide based pastes containing various essential oils against Candida albicans and comparison of its effect with zinc oxide eugenol. Indian J Med Res Pharm Sci. 2016;3:60–5.
  7. Ashraf H, Samiee M, Eslami G, Ghodse Hosseini MR. Presence of Candida albicans in root canal system of teeth requiring endodontic retreatment with and without periapical lesions. Iran Endod J. 2007;2(1):24-8.
  8. Nobile CJ, Fox EP, Nett JE, Sor rells TR, Mitrovich QM, Hernday AD, et al. A recently evolved transcriptional network controls biofilm development in Candida albicans. Cell. 2012;148(1- 2):126-38.
  9. Finkel JS, Mitchell AP. Genetic control of Candida albicans biofilm development. Nat Rev Microbiol. 2011 Feb;9(2):109-18. doi: 10.1038/nrmicro24
  10. Nobile CJ, Johnson AD. Candida albicans biofilms and human disease. Annu Rev Microbiol. 2015;69:71-92.
  11. Finkel JS, Xu W, Huang D, Hill EM, Desai JV, Woolford CA, et al. Portrait of Candida albicans a dherence r egulators. P LoS P athog. 2012;8(2):e1002525.
  12. Mahasneh SA, Mahasneh AM. Probiotics: A promising role in dental health. Dent J (Basel). 2017;5(4):26.
  13. Rose M, Kragelund C, Østrup P, Kirstine M. Probiotic Lactobacillus reuteri has antifungal effects on oral Candida species in vitro. J Oral Microbiol. 2017;9(1):1–8.
  14. Widyar man AS, Lazaroni NK. Persistent Endodontics Pathogens Biofilm Inhibited by Lactobacillus reuteri Indonesian Strain. J Dent Indones. 2019;26(3):160-4.
  15. Jaiswal N, Sinha DJ, Singh UP, Singh K, Jandial UA, Goel S. Evaluation of antibacterial efficacy of Chitosan, Chlorhexidine, Propolis and Sodium hypochlorite on Enterococcus faecalis biofilm: An in vitro study. J Clin Exp Dent. 2017 Sep;9(9):e1066.
  16. Widyarman AS, Theodorea CF. Novel indigenous probiotic lactobacillus reuteri strain produces anti-biofilm Reuterin against pathogenic periodontal bacteria. Eur J Dent. 2021 Jul 24. DOI: 10.1055/s- 0041-1731591
  17. Nikoomanesh F, Roudbarmohammadi S, Roudbary M, Bayat M, Heidari G. Investigation of BCR1 gene expression in Candida albicans isolates by RT- PCR technique and its impact on biofilm formation. IEM. 2016;2(1):22–4.
  18. Ranjith K, Chakravarthy SK, Adicherla H, Sharma S, Shivaji S. Temporal expression of genes in biofilm-forming ocular Candida albicans isolated from patients with keratitis and orbital cellulitis. Invest Opthalmol Vis Sci. 2018;59(1):528-38
  19. Schweizer A, Rupp S, Taylor BN, Rollinghoff M, Schroppel K. The TEA / ATTS transcription factor CaTec1p regulates hyphal development and virulence in Candida albicans. Mol Microbiol. 2000;38(3):435-45.
  20. Nailis H, Coenye T, Nieuwerburgh F Van, Deforce D, Nelis HJ. Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by Real-time PCR. BMC Mol Biol. 2006;4(7):25.
  21. Mu Q, Tavella VJ, Luo XM. Role of Lactobacillus reuteri in human health and diseases. Front Microbiol. 2018;9:757.
  22. Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 2013;3(3):71-85.
  23. Nobile CJ, Mitchell AP. Regulation of Cell-Surface Genes and Biofilm Formation by the C . albicans Transcription Factor Bcr1p. 2005;15:1150–5.
  24. Nikoomanesh F, Roudbarmohammadi S, Roudbary M, Bayat M, Heidari G. Investigation of bcr1 gene expression in Candida albicans isolates by RT-PCR technique and its impact on biofilm formation. Infect Epidemiol Med. 2016;2(1):22–4.
  25. Lassak T, Schneider E, Bussmann M, Kurtz D, Manak JR, Srikantha T, Soll DR, Ernst JF. Target specificity of the Candida albicans Efg1 regulator. Mol Microbiol. 2011;82(3):602-18.
  26. Leng P, Lee PR, Wu H, Brown AJP. Efg1, a morphogenetic regulator in Candida albicans, is a sequence-specific DNA binding protein. J Bacteriol. 2001;183(13):4090–3.
  27. Daniels KJ, Srikantha T, Pujol C, Park YN, Soll DR. Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans. Eukaryot Cell. 2015;14(3):228-40.
  28. Mulhern SM, Logue ME, Butler G. Candida albicans transcription factor Ace2 regulates metabolism and is required for filamentation in hypoxic conditions. Am Soc Microbiol. 2013;5(12):2001–13.
  29. Karimi S, Azizi F, Nayeb-Aghaee M, Mahmoodnia L. The antimicrobial activity of probiotic bacteria Escherichia coli isolated from different natural sources against hemorrhagic E. coli O157:H7. Electron Physician. 2018;10(3):6548-53.

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