Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines

Authors

Hanisuhana Hamidon, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Muhammad Taher, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Juliana M. Jaffri, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Tg M. Tg Zakaria, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Wan MAW Sulaiman, Department of Basic Medical Science, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Deny Susanti, Department of Chemistry, Faculty of Science, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Solachuddin JA Ichwan, Faculty of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Malaysia
Zainul A. Zakaria, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia

Abstract

Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation.

Recommended Citation

Hamidon H, Taher M, Jaffri JM, Tg Zakaria TM, Sulaiman WM, Susanti D, et al. Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines. Makara J Health Res. 2016;20.