Comparative Analysis of Proteomics Biomarkers Associated with Residual Ridge Resorption Induced by Denture Wear

Authors

Rohana Ahmad, Center of Restorative Dentistry Studies, Faculty of Dentistry, Universiti Teknologi MARA, Selangor 47000, MalaysiaFollow
Ainin Sofia Mohamad Napi, Center of Restorative Dentistry Studies, Faculty of Dentistry, Universiti Teknologi MARA, Selangor 47000, MalaysiaFollow
Tong Wah Lim, Center of Restorative Dentistry Studies, Faculty of Dentistry, Universiti Teknologi MARA, Selangor 47000, MalaysiaFollow
Su Keng Tan, Centre of Oral & Maxillofacial Surgery Studies, Faculty of Dentistry, Universiti Teknologi MARA, 47000, Selangor, MalaysiaFollow
Saiful Anuar Karsani, Institute of Biological Science, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, MalaysiaFollow
Musalmah Mazlan, Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor 47000, MalaysiaFollow
Lay Kek Teh, Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Selangor 42300, MalaysiaFollow
Steven M. Morgano, Department of Restorative Dentistry, Rutgers School of Dental Medicine, New Jersey 07103, United StatesFollow
Nadim Z. Baba, Advanced Education Program in Implant Dentistry, School of Dentistry, Loma Linda University, California 92350, United StatesFollow

Abstract

Background: The biochemical bone turnover markers for residual ridge resorption (RRR) are unclear. Therefore, the present study aimed to determine the biochemical bone turnover markers associated with RRR by comparing proteomics between the compressed mucosa of denture wearers and the non-compressed mucosa of non-denture wearers.

Methods: The mucosal specimens of 11 complete-denture wearers were obtained from the alveolar ridge during surgical implant exposure for implant-retained overdentures. All denture wearers had been edentulous and worn dentures for at least 5 years. The tissues of 11 non-denture wearers were taken from the ridge during minor preprosthetic surgery. The mucosal proteins were extracted, purified, precipitated, and subsequently separated by two-dimensional gel electrophoresis for comparative proteomics. Differentially expressed proteins between the groups were analyzed by ANOVA using Progenesis SameSpots software.

Results: Comparative proteomics analysis showed significant upregulation of 78 kDa glucose-regulated protein (GRP78; +2.2 fold, p = 0.015) and lumican (+1.8 fold, p = 0.005), as well as significant downregulation of heat shock protein 27 (HSP27; −1.9 fold, p = 0.029) in the denture group.

Conclusions: Differential expression of the biochemical bone turnover markers of GRP78, lumican, and HSP27 may occur as a result of denture pressure on the mucosa. These markers may play important roles in RRR.

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Recommended Citation

Ahmad R, Mohamad Napi A, Lim T, Tan S, Karsani S, Mazlan M, et al. Comparative Analysis of Proteomics Biomarkers Associated with Residual Ridge Resorption Induced by Denture Wear. Makara J Health Res. 2021;25.