Inflammatory Bowel Disease (IBD) is a chronic inflammatory condition in the colon that includes ulcerative colitis and Crohn’s disease. Dexamethasone is a steroid anti-inflammatory drug that can be used in IBD therapy. This study aims to obtain an optimum formulation of a dexamethasone drug delivery system for IBD treatment and to investigate its release profile based on an in vitro dissolution test. Dexamethasone was formulated as a double-coated tablet in combination with a probiotic L. acidophilus and B. longum mixture (1:1). The core tablets were produced using the wet granulation method, after which they were coated with pectin 4% b/v on the inner coat and a mixture of Eudragit L100 and S100 (1:4) on the outer coat. Three different core tablet formulas were prepared by varying the concentration of probiotics at 0%, 16% and 40% (F1, F2, and F3, respectively). The cumulative drug release of F1, F2 and F3 in HCl 0.1 N pH 1.2 for 2 hours were 42.92 ± 1.55%, 39.41 ± 4.10%, and 39.39 ± 1.63%, respectively, while in the phosphate buffer pH 6.8 they were 102.83 ± 1.56%, 105.08 ± 1.70%, and 98.81 ± 3.37% respectively, after 12 hours. From the results, we conclude that all formulas could be promising candidates for developing colon-targeted drug delivery.


Alatab, S., Sepanlou, S. G., Ikuta, K., Vahedi, H., Bisignano, C., Safiri, S., & Naghavi, M. (2020). The global, regional, and national burden of inflammatory bowel disease in 195 countries and territories, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. The Lancet Gastroenterology & Hepatology, 5(1), 17-30.

Allen, L. V., Popovich, N. G., & Ansel, H. C. (2011). Ansel’s Pharmaceutical Dosage Forms and Drug Delivery Systems (9th ed.). Philadelphia: Lippincott Williams & Wilkins.

Amidon, S., Brown, J. E., & Dave, F. S. (2015). Colon-targeted oral drug delivery system: design trends and approaches. AAPS PharmSciTech, 16(4), 731-741.

Crowley, E., & Muise, A. (2018). Inflammatory bowel disease: what very early onset disease teaches us. Gastroenterology Clinic of North America, 47(4), 755-772.

Ghosh, P. K., Gupta, V. B., Gondoliya, B., & Rathore, M. S. (2010). Probiotic-assisted colon-spesific delivery of diclofenac sodium from guar gum matrix tablets: in vitro evaluation. Asian Journal of Pharmaceutics, 4(4), 173-178.

Iswandana, R., Amangkoe, E., & Isnaini, R. (2018). Tetrandrine beads using alginate/polyvinyl alcohol and alginate-carboxymethyl cellulose: Not ideal as colon-targeted dosage form. Journal of Pharmaceutical Negative Results, 9(1), 14-20.

Iswandana, R., Mutia, M. P., & Widyaningrum, F. K. (2018). Unsuccessful delivery of tetrandrine from colon-targeted dosage forms comprising alginate/hydroxypropyl methylcellulose and alginate–chitosan beads. International Journal of Applied Pharmaceutics, 10(Special Issue 1), 396-402.

Iswandana, R., Putri, K. S., Dwiputra, R., Yanuari, T., Sari, S. P., & Djajadisastra, J. (2017). Formulation of chitosan tripolyphosphate-tetrandrine beads using ionic gelation method: In vitro and in vivo evaluation. International Journal of Applied Pharmaceutics, 9(5), 109-115.

Iswandana, R., Putri, K. S., Sandiata, C. E., Triani, S., Sari, S. P., & Djajadisastra, J. (2017). Formulation of tetrandrine beads using ionic gelation method Ca-pectinate coated pH-sensitive polymers as colon-targeted dosage form. Asian Journal of Pharmaceutical and Clinical Research, 10(10), 90-95.

Iswandana, R., Putri, K. S., Wulandari, F. R., Najuda, G., Sari, S. P., & Djajadisastra, J. (2018). Preparation of calcium alginate-tetrandrine beads using ionic gelation method as colon-targeted dosage form. Journal of Applied Pharmaceutical Science, 8(5), 68-74.

Kaur, R., Gulati, M., & Singh, S. K. (2016). Role of synbiotics in polysaccharide assisted colon targeted microspheres of mesalamine for the treatment of ulcerative colitis. International Journal of Biological Macromolecules, 95, 438-450

Maestrelli, F., Cirri, M., Corti, G., Mennini, N., & Mura, P. (2007). Development of enteric-coated calcium pectinate microspheres intended for colonic drug delivery. European Journal of Pharmaceutics and Biopharmaceutics, 69, 508-518.

Ng, S. C., Kaplan, G. G., Tang, W., Banerjee, R., Adigopula, B., & Sung, J. Y. (2019). Population density and risk of inflamatory bowel disease: a prospective population-based study in 13 countries or regions in Asia-Pacific. The American Journal of Gastroenterology, 114(1), 107–115.

Oshi, M. A., Naeem, M., Bae, J., Kim, J., Lee, J., & Yoo, J. (2018). Colon-targeted dexamethasone microcrystals with pH-sensitive chitosan/alginate/Eudragit S multilayers for the treatment of inflammatory bowel disease. Carbohydrate Polymers, 198, 434-442.

Pooja, C., Naveen, M., Rathore, M. S., Jain, A., & Kumar, J. A. (2011). Probiotic assisted colon targeted drug delivery system: research scope. Asian Journal of Pharmaceutical and Clinical Research, 4, 12-15.

Prudhviraj, G., Vaidya, Y., Singh, S. K., Yadav, A. K., Kaur, P., Gulti, M., & Gowthamarajan, K. (2015). Effect of co-administration of probiotics with polysaccharide based colon targeted delivery systems to optimize site specific drug release. Europan Journal of Pharmaceutics and Biopharmaceutics, 97, 164-172.

Saez-Lara, M. J., Gomez-Llorente, C., Plaza-Diaz, J., & Gil, A. (2014). The role of probiotic lactic acid bacteria and bifidobacteria in the prevention and treatment of inflammatory bowel disease and other related diseases: a systematic review of randomized human clinical trial. BioMed Research International, 1-15.

Singh, S. K., Yadav, A. K., Prudhviraj, G., Gulati, M., Kaur, P., & Vaidya, Y. (2015). A novel dissolution method for evaluation of polysaccharide based colon spesific delivery system: a suitable alternative to animal sacrifice. Europan Journal of Pharmaceutical Science, 73, 72-80.

Thakral, S., Thakral, N. K., & Majumdar, D. K. (2013). Eudragit®: a technology evaluation. Expert Opinion Drug Delivery, 10(1), 131-149.

Zeng, A., Dong, K., Wang, M., Sun, J., Dong, Y., Wang, K., & Xing, J. (2016). Investigation of the colon-targeting, improvement on the side-effects and therapy on the experimental colitis in mouse of a resin microcapsule loading dexamethasone sodium phosphate. Drug Delivery, 23(6), 1992-2002.



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