Prolonged fructose intake can activate the renin-angiotensin-aldosterone system and enhance oxidative stress conditions, causing hypertension and hyperuricemia. This study aimed to determine the antihypertensive and antioxidant activity of Jamu B on the activity of superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) in the kidneys of Sprague Dawley rats induced by 10% fructose. Thirty rats were divided into six groups. Groups B1-B3 were Jamu groups at a dose of 0.12 g/kg BW (B1), 0.24 g/kg BW (B2), and 0.36 g/kg BW (B3); group C (positive control) was given captopril 0.1 g/kg BW, group D (negative control) was given only 10% fructose, and group E (normal control) was healthy rats. The 10% fructose was induced for eight weeks and the treatment was given at the week 9th and 10th. Jamu B reduced systolic and diastolic blood pressure at all doses, but not significantly different from the normal control group (p>0.05). The SOD activity in groups B1, B2, B3, and C was significantly different from the negative control group (pJamu B at dose of 0.12 g/kg BW; 0.24 g/kg BW; and 0.36 g/kg BW have antihypertensive and antioxidant activity in the kidneys of rats induced by 10% fructose. Nevertheless, Jamu B at a dose of 0.36 g/kg BW was the most effective as an antihypertensive and antioxidant.


Abarikwu, S. O., Njoku, R. C., Lawrence, C. J., Charles, I. A., & Ikewuchi, J. C. (2017). Rutin ameliorates oxidative stress and preserves hepatic and renal functions following exposure to cadmium and ethanol. Pharmaceutical Biology, 55(1), 2161–2169. https://doi.org/10.1080/13880209.2017.1387575

Ahmad, W., Jantan, I., & Bukhari, S. N. a. (2016). Tinospora crispa (L.) Hook. f. & Thomson: A review of its ethnobotanical, phytochemical, and pharmacological aspects. Frontiers in Pharmacology, 7(MAR), 1–19. https://doi.org/10.3389/fphar.2016.00059

Ayala, A., Munoz, M.F., & Arguelles, S. (2014). Lipid peroxidation: review article lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014,1-31. http://dx.doi.org/10.1155/2014/360438

Azab, A. E., Albasha, M. O., & Elsayed, A. S. I. (2017). Prevention of nephropathy by some natural sources of antioxidants. Yangtze Medicine, 01(04), 235–266. https://doi.org/10.4236/ym.2017.14023

Bratoeva, K., Stoyanov, G. S., Merdzhanova, A., & Radanova, M. (2017). Manifestations of renal impairment in fructose-induced metabolic syndrome. Cureus, 9(11). https://doi.org/10.7759/cureus.1826

Bulboacă, A., Bolboacă, S. D., & Suciu, H. S. (2016). Protective effect of curcumin in fructose-induced metabolic syndrome and in streptozotocin-induced diabetes in rats. Iranian Journal of Basic Medical Sciences, 19(6), 585–593.

Caliceti, C., Calabria, D., Roda, A., & Cicero, A. F. G. (2017). Fructose intake, serum uric acid, and cardiometabolic disorders: A critical review. Nutrients, 9(4), 1–15. https://doi.org/10.3390/nu9040395

Chao, W. W., & Lin, B. F. (2010). Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian). Chinese Medicine, 5, 1–15. https://doi.org/10.1186/1749-8546-5-17

Choi, H.-N., Park, Y.-H., Kim, J.-H., Kang, M.-J., Jeong, S.-M., Kim, H. H., & Kim, J.-I. (2011). Renoprotective and antioxidant effects of Saururus chinensis Baill in rats fed a high-fructose diet. Nutrition Research and Practice, 5(4), 365–369. https://doi.org/10.4162/nrp.2011.5.4.365

Da, M., Wahyuni, A. S., Dk, I. T., Azizah, T., Suhendi, A., & Saifudin, A. (2016). Antioxidant activity of Phyllanthus niruri L. herbs: in vitro and in vivo models and isolation of active compound. National Journal of Physiology Pharmacy and Pharmacology, 6(1), 32–37. https://doi.org/10.5455/njppp.2015.5.0510201575

Dennis, J. M., & Witting, P. K. (2017). Protective role for antioxidants in acute kidney disease. Nutrients, 9(7), 1-25

Dornas, W. C., Cardoso, L. M., Silva, M., MacHado, N. L. S., Chianca, D. A., Alzamora, A. C., Lima, W. G., Lagente, V., & Silva, M. E. (2017). Oxidative stress causes hypertension and activation of nuclear factor-ΰ B after high-fructose and salt treatments. Scientific Reports, 7(July 2016), 1–11. https://doi.org/10.1038/srep46051

Eff, A. R. Y., Hurit, H. E., Rahayu, S. T., Januarko, M. U., & Gita, P. (2020). Antihypertensive, antidiabetic, antioxidant and cytotoxic activities of Indonesian traditional medicine. Pharmacognosy Journal, 12(6), 1623–1629. https://doi.org/10.5530/pj.2020.12.222

Eff, A. R. Y., Hurit, H. E., Rahayu, S. T., & Lena, A. P. (2021). Investigation of Angiotensin-Converting Enzyme Inhibitory effects of Indonesian traditional medicine (Jamu). Tropical Journal of Natural Product Research, 5(April), 692–697. https://doi.org/ 10.26538/tjnpr/v5i4.17

Eff, A. R. Y., Rahayu, S. T., Mahayasih, P. G., & Januarko, M. U. (2020). Standardization of Indonesian traditional antihypertensive medicines (JAMU) through the ACE inhibitor mechanism. Pharmacognosy Journal, 12(3), 422–429. https://doi.org/10.5530/pj.2020.12.65

Farahnak, A. Golestani, A., & Eshraghian, M. (2013). Activity of superoxide Dismutase (SOD) Enzyme in the excretory-secretory products of Fasciola hepatica and F. gigantica parasites. Iranian Journal of Parasitology, 8(1), 167-170

Giribabu, N., Rao, P. V., Kumar, K. P., Muniandy, S., Swapna Rekha, S., & Salleh, N. (2014). Aqueous extract of Phyllanthus niruri leaves displays in vitro antioxidant activity and prevents the elevation of oxidative stress in the kidney of streptozotocin-induced diabetic male rats. Evidence-Based Complementary and Alternative Medicine, 2014. https://doi.org/10.1155/2014/834815

Hwang, I. S., Ho, H., Hoffman, B. B., & Reaven, G. M. (1987). Fructose-induced insulin resistance and hypertension in rats. Hypertension, 10(5), 512–516. https://doi.org/10.1161/01.HYP.10.5.512

Ighodaro, O. M., & Akinloye, O. A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287–293. https://doi.org/10.1016/j.ajme.2017.09.001

Jayakumar, T., Hsieh, C. Y., Lee, J. J., & Sheu, J. R. (2013). Experimental and clinical pharmacology of andrographis paniculata and its major bioactive phytoconstituent andrographolide. Evidence-Based Complementary and Alternative Medicine, 2013, 1-16. https://doi.org/10.1155/2013/846740

Karimani, A., Mamashkhani, Y., Moghadam Jafari, A., Akbarabadi, M., & Heidarpour, M. (2018). Captopril attenuates Diazinon-induced oxidative stress: A Subchronic Study in Rats. Iranian Journal of Medical Sciences, 43(5), 514-522.

Kim, M., Do, G. Y., & Kim, I. (2020). Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome. Korean Journal of Physiology and Pharmacology, 24(4), 319–328. https://doi.org/10.4196/KJPP.2020.24.4.319

Köseler, E., Kızıltan, G., Türker, P. F., Saka, M., Ok, M. A., Bacanlı, D., Aydos, T. R., Bayraktar, N., & Özdemir, H. (2018). The effects of glucose and fructose on body weight and some biochemical parameters in rats. Progress in Nutrition, 20(1), 46–51. https://doi.org/10.23751/pn.v20i1.5956

Kum, C., Sekkin, S., Kiral, F., & Akar, F. (2007). Effects of xylene and formaldehyde inhalations on renal oxidative stress and some serum biochemical parameters in rats. Toxicology and Industrial Health, 23(2), 115–120. https://doi.org/10.1177/0748233707078218

Nahar, B., Morshed, M. T., Kumar Paul, A., Tabassum Zabir, A., Ahmed, I., & Rahmatullah, M. (2013). Indonesian Jamu medicines-Antinociceptive activity evaluation of two herbal preparations Anrat and Donrat. American-Eurasian Journal of Sustainable Agriculture, 7(4), 435–441.

Nakagawa, T., Johnson, R. J., Andres-Hernando, A., Roncal-Jimenez, C., Sanchez-Lozada, L. G., Tolan, D. R., & Lanaspa, M. A. (2020). Fructose production and metabolism in the kidney. Journal of the American Society of Nephrology, 31(5), 898–906. https://doi.org/10.1681/ASN.2019101015

Nurul Alia Azizan. (2012). The in vivo antihypertensive effects of standardized methanol extracts of Orthosiphon stamineus on spontaneous hypertensive rats: A preliminary study. African Journal of Pharmacy and Pharmacology, 6(6), 376–379. https://doi.org/10.5897/ajpp11.362

Rosidi, A., Khomsan, A., Setiawan, B., Riyadi, H., & Briawan, D. (2016). Antioxidant potential of Temulawak. Pakistan Journal of Nutrition, 15(6), 556–560.

Sánchez-lozada, L. G., Tapia, E., Jiménez, A., Bautista, P., Nepomuceno, T., Soto, V., Ávila-casado, C., Johnson, R. J., Herrera-acosta, J., Franco, M., Sa, L. G., Jime, A., Cristo, M., and Carmen, A. (2012). Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats and renal microvascular damage in rats. American Journal of Physiology, 292, F423-F429. https://doi.org/10.1152/ajprenal.00124.2006

Sandeva, R. V., Mihaylova, S. M., Sandeva, G. N., Trifonova, K. Y., & Popova-Katsarova, R. D. (2015). Effect of high-fructose solution on body weight, body fat, blood glucose and triglyceride levels in rats. Journal of Biomedical and Clinical Research, 8(1), 5–8. https://doi.org/10.1515/jbcr-2015-0143

Tran, L. T., Yuen, Æ. V. G., & Mcneill, Æ. J. H. (2009). The fructose-fed rat : a review on the mechanisms of fructose-induced insulin resistance and hypertension. Molecular and Cellular Biochemistry, 145–159. https://doi.org/10.1007/s11010-009-0184-4

Warsinah, Baroroh, H. N., & Harwoko. (2020). Phytochemical analysis and antioxidant activity of Brotowali (Tinospora crispa L. Mier) Stem. Molekul, 15(2), 73-78. https://doi.org/10.20884/1.jm.2020.15.2.533

Yanti, A. R., Radji, M., Mun’im, A., & Suyatna, F. D. (2014). Effects of the methanol extracts Phaleria macrocarpa (Scheff) Boerl fruits on Angiotensin Converting Enzyme (ACE) activity. International Jounal of Advances in Pharmacy, Biology and Chemistry, 3(4), 912–918.

Yanti, A. R., Radji, M., Mun’Im, A., & Suyatna, F. D. (2015). Antioxidant effects of methanolic extract of Phaleria macrocarpa (Scheff.) Boerl in fructose 10%-induced rats. International Journal of PharmTech Research, 8(9), 41–47.

Zeng, X., Xi, Y., & Jiang, W. (2019). Protective roles of flavonoids and flavonoid-rich plant extracts against urolithiasis: A review. Critical Reviews in Food Science and Nutrition, 59(13), 2125–2135. https://doi.org/10.1080/10408398.2018.1439880

Zhang, D. M., Jiao, R. Q., & Kong, L. D. (2017). High dietary fructose: direct or indirect dangerous factors disturbing tissue and organ functions. Nutrients, 9(4). https://doi.org/10.3390/nu9040335

Zhang, X.F., & Tan, B.K.H. (2000). Antihyperglycaemic and anti-oxidant properties of Andrographis paniculata in normal and diabetic rats. Clinical and Experimental Pharmacology and Physiology, 27(5-6), 358-363. https://doi.org/10.1046/j.1440-1681.2000.03253x



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.