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Abstract

The declining ability of tissues to repair or replace themselves and maintain their structure and normal function is called aging. Aging is caused by free radical that can induce the activation of elastase and collagenase. Ageratum conyzoides (L.) or known as Babadotan is a medicinal plant that has been reported to be a good source of antioxidants that also inhibit aging process. The purpose of this research is to evaluate the antioxidant and antiaging properties of Babadotan Ethanolic Extract (BEE). Franswoth method was used to determine the phytochemical assay of BEE. Measurement of 2,2-diphenyl 1-picrylhydrazyl (DPPH) scavenger was used for antioxidant assay, and inhibitory of elastase and collagenase activities was used for antiaging assay. The analysis of phytochemical compounds exhibited the presence of flavonoids, alkaloids, tannins, polyphenols, steroids and triterpenoids, monoterpenoids and terpenoids, and the absence of saponin. BEE has lower activity on DPPH scavenging activity (IC50 = 80.7 µg/mL) than quercetin (IC50 = 3.25 µg/mL). BEE exhibited lower elastase and collagenase inhibitory activity (IC50 = 45.35 ± 2.2 µg/mL and 55.07 ± 1.1 µg/mL, respectively) compared to quercetin (IC50 = 11.64 ± 0.67 µg/mL and 19.91 ± 0.46 µg/mL). Overall, BEE possesses antioxidant and antiaging activities, although the activities are lower than quercetin.

References

Adam, R. S. H., Medan, M. (2005). Kulit Menua. 38(2). 1-5.

Adnyana, I. K., Abuzaid, A. S., Iskandar, E. Y., & Kurniati, N. F. (2016). Pancreatic lipase and α-amylase inhibitory potential of mangosteen (Garcinia mangostana Linn.) pericarp extract. International Journal of Medical Research & Health Sciences, 5(1), 23-28.

Afrianti, R., Nofiandi, D., Dira, D., & Ulfa, W. (2016). Pengujian efektivitas penyembuhan luka mencit diabetes melitus yang diberikan sediaan krim ekstrak etanol daun bandotan. Scientia: Jurnal Farmasi dan Kesehatan, 6(1), 50-58.

Afrianti, R., Yenti, R., & Monica, H. (2014). Pengamatan serabut kolagen pada proses penyembuhan luka dalam sediaan krim ekstrak etanol daun bandotan (Ageratum conyzoides L.). Perkembangan Terkini Sains Farmasi dan Klinik IV, 39, 40.

Alimsyah, F., Sugihartini, N., Susanti, H. (2020). Optimasi campuran ekstrak etanol buah pepaya (Carica papaya L.) dan ekstrak etanol daun kelor (Moringa oleifera) dalam krim sebagai antiaging. Jurnal Darul Azhar, 9(1), 23-29.

Amadi, B. A., Duru, M. K. C., & Agomuo, E. N. (2012). Chemical profilesof leaf, stem, root and flower of Ageratum conyzoides. Asian Journal of Plant Science and Research, 2(4), 428-432.

Bera, T. K., Chatterjee, K., & Ghosh, D. (2015). In-vitro antioxidant properties of the hydro-methanol extract of the seeds of Swietenia mahagoni (L.) Jacq. Biomarkers and Genomic Medicine, 7(1), 18-24.

Bougeois, C., Leclerc, E. A., Corbin, C., Doussot, J., Serrano, V., Vaier, J.R., Hano, C. (2016): L’ortie (Urtica dioica L.), une source de produits antioxidants et phytochimiques anti-age pour des applications en cosmetique. Comptes Rendus Chimie,19(9), 1090-1100.

Cai, Y., Luo, Q., Sun, M., & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157-2184.

Farnsworth, N. R. (1966). Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences, 55(3), 243–264.

Galati, E. M., Miceli, N., Taviano, M. F., Sanogo, R., & Raneri, E. (2001). Anti-inflammatory and antioxidant activity of Ageratum conyzoides. Pharmaceutical Biology, 39(5), 336-339.

Ikawati, M., Wibowo, A.E., Octa, N.S., & Adelina, R.(2008): Pemanfaatan benalu sebagai agen antikanker. Laporan Penelitian, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Irshad, M., & Chaudhuri, P. S. (2002). Oxidant-antioxidant system: role and significance in human body. Indian Journal of Experimental Biology, 40(11), 1233-1239

Kim, J.H., Cho, Y.H., Lee, B.C., & Pyo, H.B. (2007): Cosmetic Composition Comprising Extracts of Pterocarpus santalinus Capable of Inhibiting Activity. Korea Patent.

Mohandas, G.G., Kumaraswamy, M. (2018) Antioxidant activities of terpenoids from Thuidium tamariscellum (C. Muell.) Bosch. and Sande-Lac. a Moss. Pharmacognosy Journal, 10(4), 645-649

Mukherjee, P. K., Maity, N., Nema, N. K., & Sarkar, B. K. (2011). Bioactive compounds from natural resources against skin aging. Phytomedicine, 19(1), 64-73.

Nasrin, F. (2013). Antioxidant and cytotoxic activities of Ageratum conyzoides stems. International Current Pharmaceutical Journal, 2(2),33-37.

Nurulita, N.A., Sundhani, E., Amalia, I., Rahmawati, F., & Utami, N.N.D. (2019). Uji aktivitas antioksidan dan anti-aging body butter dengan bahan aktif ekstrak daun kelor. Jurnal Ilmu Kefarmasian Indonesia, 17(1),1-8.

Ozgen, S., Kilinc, O. K., & Selamoğlu, Z. (2016). Antioxidant activity of quercetin: A mechanistic review. Turkish Journal of Agriculture-Food Science and Technology, 4(12), 1134-1138.

Sin, B. Y., & Kim, H. P. (2005). Inhibition of collagenase by naturally-occurring flavonoids. Archives of Pharmacal Research, 28(10), 1152-1155.

Sohn, D. H., Kim, Y. C., Oh, S. H., Park, E. J., Li, X., & Lee, B. H. (2003). Hepatoprotective and free radical scavenging effects of Nelumbo nucifera. Phytomedicine, 10(2-3), 165-169.

Tambunan, A. P., Bahtiar, A., & Tjandrawinata, R. R. (2017). Influence of extraction parameters on the yield, phytochemical, TLC-densitometric quantification of quercetin, and LC-MS profile, and how to standardize different batches for long term from Ageratum conyoides L. leaves. Pharmacognosy Journal, 9(6),767-774

Taniguchi, M., Arai, N., Kohno, K., Ushio, S., & Fukuda, S. (2012). Anti-oxidative and anti-aging activities of 2-O-α-glucopyranosyl-L-ascorbic acid on human dermal fibroblasts. European Journal of Pharmacology, 674(2-3),126-131.

Thring, T. S., Hili, P., & Naughton, D. P. (2009). Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC complementary and Alternative Medicine, 9(1), 27.

Utami, S., Sachrowardi, Q. R., Damayanti, N. A., Wardhana, A., Syarif, I., Nafik, S., Arrahman, B.C., Kusuma, H.S.W & Widowati, W. (2018). Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging. Journal of Herbmed Pharmacology, 7(2),88-93

Widowati, W., Fauziah, N., Herdiman, H., Afni, M., Afifah, E., Kusuma, H.S.W, Nufus, H., Arumwardana, S., & Rihibiha, D.D. (2016). Antioxidant and anti aging assays of Oryza sativa extract, vanillin and coumaric acid. Journal of Natural Remedies, 16(3), 88-99.

Widowati, W., Rani, A.P., Hamzah, R.A., Arumwardana, S., Afifah, E., Kusuma, H.S.W., Rihibiha, D.D., Nufus, H.,& Amalia, A. (2017). Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Natural Product Sciences, 23(3),192-200.

Widowati, W., Janeva, B.W., Nadya, S., Amalia, A., Arumwardana, S., Kusuma, H.S.W., & Arinta, Y. (2018). Antioxidant and antiaging activities of Jasminum sambac extract, and its compounds. Journal of Reports in Pharmaceutical Sciences, 7(3), 270-285

Wiedow, O., Wiese, F., & Christophers, E. (1995). Lesional elastase activity in psoriasis. Archives of Dermatological Research, 287(7), 632-635.

Wuyep, P.A., Musa, H.D., Ezemokwe, G.C., Nyam, D.D., & SilaGyang, M.D. (2017). Phytochemicals from Ageratum conyzoides L. extracts and their antifungal activity against virulent Aspergillus spp. Journal of Academia and Industrial Research, 6(3),32-39.

Xu, G.H., Ryoo, I.J., Kim, Y.H., Choo, S.J., & Yoo, I.D. (2009). Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. Archives of Pharmacal Research, 32(2), 275-282.

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