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Abstract

Caffeine consumption by pregnant women at doses above 300 mg/day was suggested to cause skeletal damage. Propolis with high flavonoids concentration could increase the number of osteoblasts. This research aims to evaluate the effect of propolis and milk administration on fetal skeletal of caffeine-induced female mice (Mus musculus). Mice were divided into six groups: negative group, positive group of caffeine (a dose of 75 mg/kg BW), positive group of propolis (a dose of 1400 mg/kg BW), positive group of milk (200 ml), group D1 (caffeine 75 mg/kg BW and propolis 1400 mg/kg BW) and group D2 (caffeine 75 mg/kg BW and milk 200 ml). Data were processed using one-way ANOVA. The results showed that administration of propolis and milk on caffeine-induced mice during pregnancy does not affect the mice body weight, the number of fetuses and fetal weight significantly (P> 0.05). No skeletal defects detected in group D1 and D2 (observation with Alizarin solution) compared to the negative group. In conclusion, the admnistration of propolis at the dose of 1400 mg/kg BW and 200 ml of milk can repair skeletal damage caused by caffeine induction.

References

Almahdy A. (2012). Experimental Teratology. Padang: Andalas University Press.

Anderson HC, Hallström H, & Kihlman BA. (2005). Intake of caffeine and other metylxhantine during pregnancy and risk for adverse effect in pregnant women and their fetuses. Copenhagen: Nordic Council of Ministers.

Beck SL & Urbano CM. (1991). Potentiating effect of caffeine on the teratogenicity of acetazolamide in C57BL / 6J mice. Teratology, 44, 241-259.

Bereket C, Ozan F, Sener I, Tech M, Altunkaynak BH, & Semirgin SU. (2014). Propolis accelerates the consolidation phase in distraction osteogenesis. J Craniofac Surg, 25, 1912-1916.

Broussard CS, Louik C, Honein MA, & Mitchell AA, & National Birth Defects Prevention Study. (2010). Herbal use before and during pregnancy. Am J Obstet Gynecol, 202, 443.e1-6.

Burdock GA. (1998). Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol, 36, 347-363. doi: 10.1016 / S0278-6915 (97) 00145-2.

Burger J, Kirchner M, Bramanti B, Haak W, & Thomas M.G. (2007). Absence of the lactase-persistance-associated allele in Early Neolithic Europeans. Proc Natl Acad Sci USA, 104, 3741.

Burns RN, Chaturvedula A, Turner DC, Zhang H, & Van Den Berg CM. (2014). Population pharmacokinetic modeling of caffeine pharmacodynamic using visual analogue scales. Pharmacol Pharm, 5, 444-454.

Committee on Toxicity. (2001). COT statement on the reproductive effects of caffeine. London: Committee on Toxicity of Chemicals in Food, Consumer Products and the Environment. Retrieved from https://goo.gl/kZ1exf, 26 November 2016 (06:34).

Dillasamola D., Almahdy A, Adrul F, Biomechy OP, Noverial. (2016). The Effect of bluetooth of smartphone against radiation teratogenicity in mice fetuses. Res J Pharm Biol Chem Sci,Vol 7 No 2, 94, 1493-1498.

Drake C, Roehrs T, Shambroom J, & Roth T. (2013). Caffeine effects on sleep taken 0, 3, or 6 hours before going to bed. J Clin Sleep Med, 11, 1195-1200.

El Sohaimy SA & Masry SHD. (2014). Phenolic content, antioxidant and antimicrobial activities of egyptian and chinese propolis. Am Eurasian J Agric Environ Sci, 10, 1116-1124.

Elwakkad USA, El-Elshamy KA, & Sibaii H. (2008). Fish liver oil and natural products propolis as protective against the effect of the anti-epileptic drug valproate on immunological markers of bone formation in rats. Epilepsy Res, 80, 47-56.

European Food and Safety Authority. (2015). Pharmacokinetic and pharmacodinamic effects of caffeine (papers scientific speech). EFSA Stakeholders Meeting on the Safety of Caffeine, March 5th 2015, Brussels.

Franz. (2008). Healthy with Bee Therapy (Apitherapy). Jakarta: PT. Elex Media Komputindo.

Guharaj PV & Chandran MR. (2003). Forensic Medicine 2th edition. India: Orient Longman Ltd.

Guyton AC. (1990). Medical Physiology (5th ed.), Translators: A. Dharma. Jakarta: Book Medical Publishers EGC.

Harbinson RD. (2001). The Basic science of poison cassaret and doull’s toxicology. New York: Macmillan Publishing Co. Inc.

Herbold B. (1985). Micronucleus test on the mouse to Evaluate for mutagenic effects. Report No. 14102E (Study Nos. T6019042 and T8019675). Institute of Toxicology, Pharmaceutical Division, Bayer AG, Wuppertal, Germany. Submitted to WHO by Bayer AG, Leverkusen, Germany (report No. 74 164).

Jeong TC, Noh K, Oh DG, Nepal MR, Jeong KS, Kang W, Kang MJ, Jeong HG. (2016). Pharmacokinetic interaction of chrysin with caffeine in rats. Biomol Ther, 24, 446-452.

Katzung BG. (2004). Basic and clinical pharmacology (9RD ed.). San Francisco: Mc Graw-Hill Medical.

Kementerian Kesehatan RI. (1979). Indonesian Pharmacopoeia : The third edition. Jakarta: Kementerian Kesehatan RI.

Lee KH. (2009). Medical students’ use of caffeine for academic purposes “and their knowledge of its benefits, side-effects and withdrawal symptoms. S Afr Fam Pract, 51, 322-327.

Lu FC. (1995). Basic Toxicology (2nd ed.). Translator: E. Nugroho. Chicago: University of Chicago Press.

Manson JM, Zenict H, & Costlow R.D. (1982). Teratology test methods for laboratory animals. New York: Revent Press.

Marcucci MC, Ferreres F, Viguera GC, Bankova VS, De Castro SL, Dantas AP, Valente PHM, and Paulino N. (2001). Phenolic compounds from brazilian propolis with pharmacological activities. J Ethnopharmacol, 74, 105-112.

Marquinaa AC, Tarínb JJ, & Cano A. (2013). Review: The impact of coffee on health. Maturity, 75, 7-21.

Masyitha D. (2006). Struktur mikroskopik tulang mandibula pada tikus ovariektomi dan pemberian pakan rasio fosfat/kalsium tinggi. Media Kedokteran Hewan, 22 (2).

Mehta T. (2007). Milk intake in pregnancy. CMAJ Suppl, 176, 1460.

Mesas AE, Mun~oz LML, Artalejo FR, & Garcia EL (2011). The effect of coffee on blood pressure and cardiovascular disease in hypertensive individuals: A systematic review and meta-analysis. Am J Clin Nutr,94, 1113-26.

Mose T, Kjaerstad MB, Mathiesen L, Nielsen JB, Edelfors S, & Knudsen LE. (2008). Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system. J Toxicol Environ Health, 71, 984-991.

Nawrot P, Jordan S, Eastwood J, Rotstein J, Hugenholtz A, & Feeley M. (2002). Effects of caffeine on human health. Food Addit Contam, 20 (1), 1-30.

Nishimura H & Nakai K. (1960). Congenital malformation in off spring of mice treated with caffeine. Proc Soc Exp Biol Med, 104, 140-142.

Organization of Teratology Information Specialists (OTIS). (2015). Caffeine. Mother to Baby. Retrieved from http://mothertobaby.org/fact-sheets/caffeine-pregnancy/pdf/, October 24, 2016 (08:12) Paul ST. (2010). Drinking milk during pregnancy may lower baby’s risk of MS. In: American Academy of Neurology’s 62nd Annual Meeting. Canada: Toronto.

Santoso HB. (2004). Structural abnormalities anatomy fetus mice due to caffeine. Bioscientiae, 1, 23-30.

Sartika N, Narmada IB, Sjamsudin J. (2013). Effect of propolis extracts on alveolar bone around osteoblasts expression during orthodontic tooth movement in cavia cobaya. Orthodontic Dental Journal, 4, 5-9.

The US Food and Drug Administration Oakridge National Laboratory. (2010). Caffeine Intake by the US Population. USA: FDA. Weinsier RL & Krumdieck CL. (2000). Diary foods and bone health: examination of the evidence. Am J Clin Nutr, 72, 681-689. Welsh EJ, Bara A, Barley E, & Cates C.J., (2001). Caffeine for asthma. Cochrane Database of Systematic Reviews, Issue 4.

Weng X, Odouli R, & Li DK. (2008). Maternal caffeine consumption during pregnancy and the risk of miscarriage: A prospective cohort study. Am J Obstet Gynecol, 198, e1-8.

Wilson JG, Fraser FC. (1973). Handbook of Teratology 1-4. New York and London: Plenum Press

Winarno. (1993). Food, Nutrition, and Consumer Technology. Jakarta: PT. Gramedia Pustaka General.

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