•  
  •  
 

Abstract

The combined use of natural materials and antibiotic compound is one of treatment that can be done against infections caused by bacteria. This combination is expected to be more potent to inhibit the bacteria with lower side effects. FICI (Fractional Inhibitory Concentration Index) is an index that can indicate the activity of a combination of natural ingredients and antibiotic compounds. FICI would indicate that the combination has a synergistic effect, additive, indifferent or antagonistic. The aim of this study is to determine the FICI to the combination of Aloe vera leaves skin (Aloe vera (L.) Burm.f.) ethanol extract and gentamicin sulphate againts Escherichia coli. FICI was determined by disc diffusion method. Combination of Aloe vera leaves skin (Aloe vera(L.) Burm.f.) ethanol extract and gentamicin sulphate with concentration of 1.25 mg/mL + 2,5 µg/mL was not form zone of inhibition; while the concentration of 2.5 mg/ml + 5 µg/ mL formed zone of inhibition of 6.95 mm; 6.75 mm; and 6.65 mm. The results showed that the combination of Aloe vera leaves skin (Aloe vera (L.) Burm.f.) ethanol extract and gentamicin sulphate against the Escherichia coli has the indifference effect on Escherichia coli, and FICI to this combination is 2.

Bahasa Abstract

Penggunaan kombinasi senyawa bahan alam dan antibiotik merupakan salah satu pengobatan yang dapat dilakukan terhadap infeksi yang disebabkan bakteri. Kombinasi ini diharapkan mampu menghambat bakteri lebih poten dan efek sampingnya rendah. FICI (Fractional Inhibitory Concentration Index) adalah indeks yang dapat menunjukkan aktivitas dari kombinasi senyawa bahan alam dan antibiotik. Nilai FICI akan menunjukkan bahwa kombinasi memiliki efek sinergis, aditif, indiferen, atau antagonis. Penelitian ini bertujuan untuk menentukan nilai FICI kombinasi ekstrak etanol kulit daun lidah buaya (Aloe vera (L.) Burm.f.) dan gentamisin sulfat terhadap bakteri Escherichia coli. Penentuan nilai FICI dilakukan dengan metode disk difusi. Konsentrasi kombinasi ekstrak etanol kulit daun lidah buaya (Aloe vera (L.) Burm.f.) dan gentamisin sulfat yang digunakan yaitu 1,25 mg/mL + 0,0025 mg/mL tidak membentuk zona hambat; dan 2,5 mg/mL + 0,005 mg/mL membentuk zona hambat sebesar 6,95 mm; 6,75 mm; dan 6,65 mm. Hasil ini menunjukkan bahwa kombinasi ekstrak etanol kulit daun lidah buaya (Aloe vera (L.) Burm.f.) dengan gentamisin sulfat terhadap Escherichia coli memiliki efek indiferen dengan nilai FICI 2.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.