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Abstract

Chitinase is an enzyme that degrades chitin on β-1,4-acetamido-2-deoxy-D-glycoside bound to produce Chitin oligosaccharide or its soluble monomer, N-acetylglucosamine (NAG). Chitinase are usually produced naturally by chitinolytic microorganism that are found in soil or water environment. Chitinase have been widely used for biocontrol agents of plant pests. The product from chitinase hydrolysis can be used as antiinflammatory agent and anti hyperpigmentation. This research was aimed to screen and evaluate the chitinolytic ability of 9 isolate. The best isolate was the one which produced high chitinolytic activity and hydrolyzed chitin into NAG. 9 isolate were examined and BPPTCC-2 was the best isolate which produced highest level of chitinolytic activity 0.1780 U/ml. The chitinase was able to hydrolyzed chitin resulting high yields of NAG 11.5% as the only product.

Bahasa Abstract

Kitinase merupakan enzim yang mendegradasi kitin pada ikatan β-1,4-asetamido-2-deoksi-D-glikosida menjadi produk turunannya yaitu kitin oligosakarida atau monomer N-asetilglukosamin (NAG). Enzim kitinase dihasilkan oleh mikroorganisme kitinolitik yang sebagian besar terdapat di lingkungan tanah dan air. Kitinase dimanfaatkan pada bidang pertanian sebagai agen biokontrol. Produk hasil hidrolisisnya dimanfaatkan dalam bidang kesehatan dan kosmetik sebagai suplemen anti inflamasi dan anti hiperpigmentasi.Penelitian kali ini bertujuan untuk skrining dan evaluasi kemampuan 9 bakteri isolat dalam menghasilkan kitinase. Isolat yang terbaik adalah isolat yang menghasilkan kitinase dengan aktivitas yang tinggi dan mampu menghidrolisis kitin menjadi monomer NAG. Dari 9 isolat bakteri tersebut, bakteri BPPTCC-2 merupakan bakteri yang terpilih karena mampu menghasilkan kitinase dengan aktivitas tertinggi yaitu 0,1780 U/mL. Kitinase tersebut dapat menghidrolisis substrat kitin dan menghasilkan NAG sebagai produk tunggal dan dengan rendemen NAG paling tinggi yaitu 11,5%.

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