Abstract
The use of temephos as larvicides in disease prevention Dengue Hemorrhagic Fever (DHF) in Indonesia has been going on for 40 years. Larviciding very long led the resistance to temephos. Cloves, one of native Indonesia plant contains eugenol that are toxic to the insect’s body. This study aims to examine the effectiveness of Zanzibar clove leaf essential oil compared to temephos in killing the larvae of Aedes aegypti. This study uses experimental laboratory research design. The samples are larvae of Aedes aegypti stage III. The mosquito larvae were classified into six groups, each given a clove leaf essential oil with different concentrations. Next will be observed the number of larvae that die after 24 hours. Based on statistical test p-value p < 0.05 it indicates there are differences in the average mortality in the test group, and post hoc analysis showed the test group with 100 ppm of essential oil is not a significant difference with temephos. This study suggests the essential oil of clove leaf varieties of Zanzibar has the same effectiveness with temephos in killing the larvae of Aedes aegypti.
Bahasa Abstract
Penggunaan temephos sebagai larvasida dalam pencegahan penyakit demam berdarah dengue (DBD) di Indonesia sudah berlangsung selama 40 tahun. Penggunaan larvasida yang sangat lama ini memunculkan adanya resistensi terhadap temephos. Cengkeh, salah satu tanaman asli Indonesia mengandung eugenol yang bersifat racun pada tubuh serangga. Penelitian ini bertujuan untuk meneliti efektifitas minyak atsiri daun cengkeh Zanzibar dibandingkan dengan temephos dalam membunuh larva nyamuk Aedes aegypti. Penelitian ini menggunakan desain penelitian ekperimental laboratorik. Sampel yang digunakan adalah larva nyamuk A aegypti instar III. Larva nyamuk dikelompokan kedalam 6 kelompok, masing- masing diberikan minyak atsiri daun cengkeh dengan konsentrasi yang berbeda-beda. Selanjutnya akan diamati jumlah larva setelah 24 jam. Berdasarkan uji stastistik didapatkan nilai p < 0,05 hal ini menandakan terdapat perbedaan rata-rata kematian pada kelompok uji, dan analisa post hoc menunjukan pada kelompok uji dengan 100 ppm minyak atsiri tidak terdapat perbedaan yang signifikan dengan temephos. Dari penitian ini didapatkan minyak atsiri daun cengkeh varietas Zanzibar memiliki efektifitas larvasida yang sama dengan temephos dalam membunuh larva nyamuk A aegypti.
References
Arivia, S., Kurniawan, B., Zuraida, R. (2013). Efek larvasida ekstrak daun lidah buaya (Aloe vera) terhadap larva aedes aegypti instar III. Medical Journal of Lampung University, 137–146
Cania, E., Setyaningrum, E. (2013). Uji efektivitas larvasida ekstrak daun legundi (Vitex trifolia) terhadap larva aedes aegypti. Medical Journal of Lampung University, 2(4), 52–60
Faraco, A., Araujo, D. O., Ribeiro-paes, J. T., Deus, J. T. De. (2016). Larvicidal activity of Syzygium aromaticum (L.) Merr and Citrus sinensis ( L .) Osbeck essential oils and their antagonistic effects with temephos in resistant populations of Aedes aegypti, Memórias do Instituto Oswaldo Cruz, 111(7), 443-449
Fayemiwo, K. A., Adeleke, M. A., Okoro, O. P., Awojide, S. H., Olufemi, I., Awoniyi. (2014). Larvicidal efficacies and chemical composition of essential oils of Pinus sylvestris and Syzygium aromaticum against mosquitoes. Asian Pacific Journal of Tropical Biomedicine, 4(1), 30–34
Fuadzy, H., Hodijah, D. N., Jajang, A., & Widawati, M. (2015). Kerentanan larva aedes aegypti terhadap temefos di tiga kelurahan endemis demam berdarah dengue kota sukabumi. Buletin Penelitian Kesehatan, 43(1), 41–46
Gunandini G, Kardinan, A. (2008). Pengaruh ekstrak kemangi (Ocimum basilicum forma citratum) terhadap perkembangan lalat rumah (Musca domestica) ( L .). Jurnal Entomologi Indonesia, 5(1), 36–44
Istiana, I., Heriyani, F., & Isnaini, I. (2012). Resistance status of Aedes aegypti larvae to temephos in West Banjarmasin. Jurnal Buski, 4(2)
Kementrian Kesehatan. (2014). Profil Kesehatan Indonesia Tahun 2013. Jakarta: Kementerian Kesehatan RI
Lariska, S. S., Martini, & Lintang Dian, S. (2016). Status resistensi larva Aedes aegypti (Linnaeus) terhadap Temephos (Studi di Kelurahan Jatiasih Kecamatan Jatiasih Kota Bekasi Provinsi Jawa Barat). Jurnal Kesehatan Masyarakat, 4(1), 142–152
Manimaran, A., Jee, M. M., Cruz, J., Muthu, C., Vincent, S., & Ignacimuthu, S. (2012). Larvicidal and knockdown effects of some essential oils against Culex quinquefasciatus Say , Aedes aegypti ( L .) and Anopheles stephensi ( Liston ). Advances in Bioscience and Biotechnology, 3, 855-862
Mohammad Nazrul Islam Bhuiyan. (2012). Constituents of the essential oil from leaves and buds of clove (Syzigium caryophyllatum (L.) Alston). African Journal of Pharmacy and Pharmacology, 6(16), 1260–1263
Pratiwi, A. (2014). Studi deskriptif penerimaan masyarakat terhadap larvasida alami. Jurnal Ilmu Kesehatan Masyarakat, 3(2), 1–10
Taher, D. M., & Papuangan, N. (2015). Potensi cengkeh (Syzygium aromaticum) varietas Afo sebagai larvasida alami nyamuk Anopheles subpictus dan Aedes aegypti, Biodiversitas Indonesia, 1(6), 1478–1482
Tennyson, S., Samraj, D. A., Jeyasundar, D., Chalieu, K., College, M. C., & Nadu, T. (2013). Larvicidal Efficacy of Plant Oils Against the Dengue Vector Aedes aegypti ( L .) ( Diptera : Culicidae ). Middle-East Journal of Scientific Research, 13(1), 64-68
Recommended Citation
Pamungkas, Ridzmullah Wishnu; Syafei, Neneng Syarifah; and Soeroto, Arto Yuwono
(2016)
"Perbandingan Efek Larvasida Minyak Atsiri Daun Cengkeh (Syzygium aromaticum L.) Varietas Zanzibar dengan Temephos terhadap Larva Nyamuk Aedes aegypti,"
Pharmaceutical Sciences and Research: Vol. 3:
No.
3, Article 4.
DOI: 10.7454/psr.v3i3.3566
Available at:
https://scholarhub.ui.ac.id/psr/vol3/iss3/4
Included in
Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacy and Pharmaceutical Sciences Commons, Pharmaceutics and Drug Design Commons