Abstract
Mangosteen rind possesses the ability to conduct antibacterial activity. Under the development relating to drug delivery system, SNEDDS were known to boost the penetration of active compound. The aim of this research is to compare the effectiveness of the antibacterial activity at both SNEDDS preparation and the ethanol extract of mangosteen rind as the antibacteria against the bacteria that cause diabetic ulcers with the most prevalence whether it was Gram positive or Gram negative bacteria like Proteus mirabilis and Staphylococcus epidermidis. The research started by maceration process with the crude extract as the result continued with the phytochemical screening which subsequently prepared as SNEDDS preparations. The SNEDDS preparation prepared earlier were then analyzed to see the content of the active compound using spectrophotometer UV-Vis and the measuring of antibacteria activity with bacteria growth inhibitory parameter at ethanol extract SNEDDS preparations of mangosteen rind then compared to ciprofloxacin and were analyzed the data afterwards using ANOVA. The results shows that SNEDDS preparation of mangosteen extract have an activity against both typed of bacteria that cause diabetic ulcers shows by significant differences between both group which is P.mirabilis bacteria group and S.epidermidis bacteria group in ANOVA test with significant number of 0,000 and shows significant differences between both group.
Bahasa Abstract
Kulit buah manggis memiliki aktivitas antibakteri. Dalam pengembangan sistem penghantaran obat, SNEDDS memiliki banyak keuntungan untuk meningkatkan penetrasi senyawa aktif. Tujuan dari penelitian ini adalah untuk mengetahui perbandingan efektivitas antibakteri dari sediaan SNEDDS dan ekstrak etanol kulit buah manggis sebagai antibakteri terhadap bakteri penyebab ulkus diabetik dengan prevalensi tertinggi baik bakteri Gram positif maupun bakteri Gram negatif yaitu Proteus mirabilis dan Staphylococcus epidermidis. Pada penelitian ini diawali dengan maserasi sehingga diperoleh ekstrak kental dilanjutkan skrining fitokimia, kemudian dipersiapkan menjadi bentuk sediaan SNEDDS. Sediaan obat yang sudah disiapkan kemudian dianalisis kandungan senyawa aktif dengan menggunakan spektrofotometer UV-Vis dan pengukuran daya aktivitas antibakteri dengan parameter daya hambat pertumbuhan bakteri terhadap sediaan SNEDDS ekstrak etanol kulit manggis dibandingkan dengan siprofloksasin kemudian data dianalisis menggunakan ANOVA. Hasil penelitian menunjukkan bahwa sediaan SNEDDS ekstrak kulit manggis memiliki aktivitas terhadap kedua jenis bakteri penyebab ulkus diabetik terlihat adanya perbedaan bermakna pada tiap kelompok pada bakteri P. mirabilis dan S. epidermidis yang ditunjukkan dengan anova dengan angka signifikansi pada 0,000.
References
Moongkarndi, P., et al. (2004). Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. Journal of Ethnopharmacology, 90, 161–166.
Nazzal, S., dan Khan, M.A. (2002).Response surface methodology for the optimization of ubiquinone self-nanoemulsified drug delivery system. AAPS PharmSciTech,3, 23–31.
Pedraza-Chaverri, J., et al. (2008). Medicinal properties of mangosteen (Garcinia mangostana). Food and Chemical Toxicology, 46, 3227–3239.
Shafiq-un-Nabi, S., et al., dkk. (2007). Formulation development and optimization using nanoemulsion technique: a technical note. AAPS pharmscitech, 8, E12–E17.
Susanto Y dkk. (2009). Efek serbuk biji kopi robusta (Coffea robusta Lindl Ex de Willd), jkm terhadap waktu penutupan luka pada mencit jantan galur Balb/c yang diinduksi aloksan. JKM, 8(2),121-126.
Sutjahjo A. (2013). Kuman dan Uji kepekaan Antibiotik, Indonesian Journal of Clinical Pathology and medical laboratory, 20(1).
Vishnu Priya et al. (2010). Antimicrobial activity of pericarp extract of Garcinia mangostana Linn, International Journal of Pharma Sciences and Research, 1(8), 278-281.
Weecharangsan, W., et al. (2006). Antioxidative and neuro protective activities of extracts from the fruit hull of mangosteen (Garcinia mangostana Linn.). Journal Medical Princ Practice,15,281-287.
Recommended Citation
Sari, Rafika; Pratiwi, Liza; and Apridamayanti, Pratiwi
(2016)
"Efektivitas SNEDDS Ekstrak Kulit Manggis Terhadap Bakteri P. mirabilis dan S. epidermidis yang Terdapat pada Ulkus Diabetik,"
Pharmaceutical Sciences and Research: Vol. 3:
No.
3, Article 3.
DOI: 10.7454/psr.v3i3.3287
Available at:
https://scholarhub.ui.ac.id/psr/vol3/iss3/3
Included in
Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacy and Pharmaceutical Sciences Commons, Pharmaceutics and Drug Design Commons
