Abstract
Ethanolic extract of bacang mango leaves (Mangifera foetida L.) decreased iron concentration in blood Sprague dawley rats that had been induced iron overload. The aim of this experiment was to determine acute toxicity (LD50) value of ethanolic extract of M.foetida by weil method and its effect to blood component. This study was conducted by employing a complete random design using 25 male and 25 female mice of DDY strain which devided into 5 groups. The first group until fourth group were administered ethanolic extract of M.foetida with dose variation which suspension in aquadest orally. The fifth group was control that administered aquadest. The LD50 was determined by the total of death on all group. LD50 value of the extract showed no death in the biggest doses (13.013 g/kg). The examination was continued with measured blood count (erythrocites, trombocytes, leukocytes, and haemoglobin concentration). It was shown that the extract at dose 1.626; 3.253; 6.506 and 13.013 g/kg unchanged the blood count measurement.
Bahasa Abstract
Ekstrak etanol daun mangga bacang (Mangifera foetida L.) dapat menurunkan konsentrasi besi dalam darah tikus galur Sprague dawley yang telah diinduksi dengan besi berlebih (iron overload). Tujuan penelitian ini adalah menetukan nilai toksisitas akut (LD50) dari ekstrak etanol M.foetida dengan menggunakan metode weil dan pengaruhnya terhadap komponen dalam darah. Penelitian ini menggunakan desain randomisasi secara acak. Pada penelitian ini menggunakan 25 mencit jantan dan 25 mencit betina galur DDY yang dibagi menjadi 5 kelompok. Kelompok pertama sampai keempat merupakan kelompok yang diberikan ekstrak etanol M.foetida dengan dosis bertingkat yang disuspensikan dengan aquades yang diberikan secara oral. Kelompok kelima merupakan kelompok kontrol yang diberikan aquades. Nilai LD50 ditentukan dengan cara menghitung jumlah kematian total dari semua kelompok. Hasil LD50 dari ekstrak menunjukkan tidak adanya kematian sampai pada dosis terbesar (13,013 g/kg). Pengukuran dilanjutkan dengan penghitungan komponen darah (eritrosit, trombosit, leukosit, dan konsentrasi hemoglobin). Hasil menunjukkan bahwa ekstrak pada dosis 1,626; 3,253; 6,506 and 13,013 g/kg tidak mengubah dari jumlah atau konsentrasi komponen darah.
References
Anonim. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Cetakan Pertama. Jakarta: Departemen Kesehatan Republik Indonesia Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional.
Brittenham GM. (2003). Iron chelators and iron toxicity. Alcohol. 2003;30:151-8.
Anonim. (1995). Farmakope Indonesia, Ed IV. Jakarta: Departemen Kesehatan Republik Indonesia.
Franck, C Lu. (1995). Toksikologi Dasar: Asas, Organ Sasaran, dan Penilaian Risiko Edisi 2 (Edi Nugroho, Penerjemah.). Jakarta: UI Press.
Gonza´lez JE, Rodrı´guez MD, Rodeiro I, Morffi J, Guerra E, Leal F, et al. (2007). Lack of in vivo embryotoxic and genotoxic activities of orally administered stem bark aqueous extract of Mangifera indica L. (Vimang®). Food and Chemical Toxicology, 45, 2526–2532.
Harmita & Maksum Radji. (2005). Buku Ajar Analisis Hayati Ed.2. Depok: Departemen Farmasi FMIPA UI.
Haśková P, Koubková L, Vávrová A, Macková E, Hruśková K, Kovaŕíková P, et al. (2011). Comparison of various iron chelators used in clinical practice as protecting agents against catecholamine-induced oxidative injury and cardiotoxicity. Toxicology, 289:122– 31.
Liu ZD, Hider RC. (2002). Design of iron chelators with therapeutic application. Coordination Chemistry Reviews, 232:151-71.
Nishiyama K, Choi YA, Honsho C, Eiadthong W, Yonemori K. (2006). Application of genomic in situ hybridization for phylogenetic study between Mangifera indica L. and eight wild species of Mangifera. Scientia Horticulturae, 110:114–17.
Pardo-Andreu G, Delgado R, Velho JA, Curti C, Vercesi AE. (2005). Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibit mitochondrial lipid peroxidation induced by Fe2+-citrate. European Journal of Pharmacology, 513:47-55.
Pardo-Andreu GL, Cavalheiro RA, Dorta DJ, Naal Z, Delgado R, Vercesi AE, et al. (2007). Fe(III) shifts the mitochondria permeability transition-eliciting capacity of mangiferin to protection of organelle. The Journal of Pharmacology and Experimental Therapeutics, 320:646-53.
Purwaningsih EH, Hanani E, Amalia P, Krisnamurti DG. (2011). The chelating effect of Mangifera foetida water extract on serum thalassemic patient. J Indon Med Assoc, 61(8):321-5.
Rodeiro I, Donato MT, Jime´nez N, Garrido G, Delgado R, Go´mez-Lecho´n MJ. (2007). Effects of Mangifera indica L. aqueous extract (Vimang) on primary culture of rat hepatocytes. Food and Chemical Toxicology, 45,2506–12.
Siah CW, Trinder D, Olynnyk JK. (2005). Iron overload. Clinica Chimica Acta, 358:24-36.
Soebrata G. (2001). Penentuan Laboratorium Klinis. Jakarta:Dian Rakyat.
Szuber N, Buss JL, Soe-Lin S, Felfly H, Trudel M, Ponka P. (2008). Alternative treatment paradigm for thalassemia using iron chelators. Experimental Hematology, 36,773-85.
Wong C, Richardson DR. (2003). β-Thalassaemia: emergence of new and improved iron chelators for treatment. The International Journal of Biochemistry & Cell Biology, 35, 1144-9.
Recommended Citation
Wahyuni, Tri; Sari, Santi Purna; Estuningtyas, Ari; and Freisleben, HJ
(2015)
"Toksisitas Ekstrak Etanol Mangifera foetida L. sebagai Pengkelat Besi Ditinjau dari LD50 dan Komponen Sel Darah,"
Pharmaceutical Sciences and Research: Vol. 2:
No.
3, Article 2.
DOI: 10.7454/psr.v2i3.3342
Available at:
https://scholarhub.ui.ac.id/psr/vol2/iss3/2
Included in
Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacy and Pharmaceutical Sciences Commons, Pharmaceutics and Drug Design Commons
