Abstract
Javanese women often consume kunir asam to relieve dysmenorrhea symptom. It is a traditional herbal medicine which is conventionally prepared from curcuma domestica. One of the active compound of curcuma domestica is curcumin. The purpose of this research is to determine the amount of curcumin available in kunir asam selling in daily market of Kota Gede. Curcumin level in daily batches consistency was also evaluated. Curcumin analysis was conducted by qualitative and quantitative tests by TLC densitometry method. A total of 50.0 ml kunir asam was extracted with 25.0 ml of chloroform, and then the mixture was dried by evaporation. The dried residue of extract was then reconstituted in 5.0 ml of 96% ethanol. 5 μl of sample supernatant and standard solutions spotted on the plates of silica gel 60 F254 that has been activated previously. The organic solvent mixture comprise of chloroform: ethanol: glacial acetic acid (94:5:1) was used as the mobile phase for elution, and the spots were then analyzed by using densitometry at wavelength of 425 nm. Curcumin levels was calculated by inputting the size of spot area data into the standard curve linear regression equation of curcumin concentration versus the size of spot area. The data analysis was performed by normality test Kolmogorov-Smirnov test, Levene test of homogeneity followed by Post Hoc / Test LSD (Least Significant Difference) or Mann Whitney. TLC analysis conducted on four samples of “kunir asam” displayed a disparities on the curcumin level between the three days of sampling in a row. Statistical analysis of the curcumin content in traditional herbal medicine of “kunir asam” samples showed that the levels were not consistent in the three days of the examination. Curcumin levels in all four samples were also shows a significant difference in variations.
Bahasa Abstract
Wanita jawa sering mengkonsumsi jamu kunir asam sebagai obat dismenorea. Salah satu zat aktif jamu tradisional kunir asam adalah kurkumin. Tujuan penelitian ini adalah mengetahui kadar kurkumin dalam jamu tradisional kunir asam di pasar Kota Gede serta melihat konsistensinya. Penetapan kadar kurkumin kualitatif dan kuantitatif dilakukan dengan metode KLT densitometri. Sebanyak 50,0 ml jamu tradisional kunir asam diekstraksi dengan 25,0 ml kloroform, diuapkan hingga kering, lalu larutkan dengan 5,0 ml etanol p.a. Sebanyak 5 μl sampel dan larutan standar ditotolkan pada lempeng silika gel 60 F254 yang sudah diaktifkan, dikembangkan dengan fase gerak kloroform : etanol : asam asetat glasial (94 : 5 : 1), dianalisis menggunakan KLT densitometri pada panjang gelombang 425 nm. Kadar kurkumin dihitung dengan memasukkan data AUC ke persamaan regresi linier kurva baku kurkumin. Analisis data dan uji normalitas dilakukan dengan uji Kolmogorov-Smirnov, homogenitas uji Levene dilanjutkan dengan Uji LSD (Least Significant Difference) atau Kruskall-Wallis. Analisis statistik kandungan kurkumin jamu tradisional kunir asam menunjukkan bahwa kadar tidak konsisten pada tiga hari pemeriksaan. Kadar kurkumin pada keempat sampel juga menunjukkan variasi yang signifikan (p<0.01).
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Recommended Citation
Yusuf, Fitrilya Mustikahati and Nurkhasanah, Nurkhasanah
(2015)
"Evaluasi Kadar Kurkumin dalam Jamu Tradisional Kunir Asam yang Dijual di Pasar Kota Gede Bulan Februari 2015,"
Pharmaceutical Sciences and Research: Vol. 2:
No.
3, Article 1.
DOI: 10.7454/psr.v2i3.3342
Available at:
https://scholarhub.ui.ac.id/psr/vol2/iss3/1
Included in
Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacy and Pharmaceutical Sciences Commons, Pharmaceutics and Drug Design Commons
