Enzim Papain: Aspek Green Chemistry pada Reaksi Knoevenagel

Authors

Laurentius Haryanto, Departemen Kimia, FMIPA, Universitas Indonesia, Depok. 16424Follow
Antonius Herry Cahyana, Departemen Kimia, FMIPA, Universitas Indonesia, Depok. 16424

Abstract

Green chemistry aspect is the chemical approach that has been studied in the past two decade. One of the principles is the development of green synthesis process that is friendly for the environment. This research showed that papain can be used as catalyst for Knoevenagel reaction with 3 kinds of substituted-benzaldehyde and malononitrile as substrates in aqueous medium. The best reaction condition with 80% yield was reached by utilizing of 25 mg papain/mmol substrate. Reaction was conducted at ambient temperature and pressure for 30 min. Products were as yellowish to yellow needle crystals and successfully characterized by melting point, UV-Vis, IR, mass spectra, and 13C & 1H-NMR, named as 2-(4-hydroxybenzylidene)-malononitrile); 2-(3-hydroxybenzylidene)-malononitrile); and 2-(4-hydroxy-3-methoxybenzylidene)-malononitrile).

Bahasa Abstract

Aspek green chemistry merupakan pendekatan kimia yang banyak digunakan dalam dua dekade belakangan ini. Salah satu prinsipnya adalah pengembangan metode sintesis yang lebih “hijau”. Hasil penelitian ini menunjukkan bahwa papain terbukti dapat mengkatalisis reaksi Knoevenagel untuk 3 macam senyawa benzaldehida tersubstitusi dan malononitrile yang dilangsungkan dalam pelarut air. Kondisi terbaik, dengan rendemen sekitar 80%, diperoleh pada penggunaan papain sebanyak 25 mg/mmol substrat. Reaksi dilangsungkan pada suhu dan tekanan ruang selama 30 menit. Tiga produk yang diperoleh berbentuk kristal jarum berwarna kekuningan hingga kuning dan telah berhasil dikarakterisasi, melalui penentuan titik leleh, spektrum UV-Vis, IR, Massa, serta 13C dan 1H-NMR, sebagai senyawa 2-(4-hydroxybenzylidene)-malononitrile); 2-(3-hydroxybenzylidene)-malononitrile); dan 2-(4-hydroxy-3-methoxybenzylidene)-malononitrile).

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Recommended Citation

Haryanto, Laurentius and Cahyana, Antonius Herry (2015) "Enzim Papain: Aspek Green Chemistry pada Reaksi Knoevenagel," Pharmaceutical Sciences and Research: Vol. 2: No. 2, Article 2.
DOI: 10.7454/psr.v2i2.3320
Available at: https://scholarhub.ui.ac.id/psr/vol2/iss2/2