Skrining Fitokimia dan Aktivitas Antioksidan Fraksi Etanol Alga Merah Eucheuma spinosum

Authors

Bina Lohita Sari, Program Studi Farmasi, FMIPA, Universitas PakuanFollow
Nurulia Susanti, Program Studi Farmasi, FMIPA, Universitas Pakuan
Sutanto Sutanto, Program Studi Kimia, FMIPA, Universitas Pakuan

Abstract

The red alga, Eucheuma spinosum from South Bangka Waters was shown to contain antioxidant activity. Natural antioxidants from algae are known to play an important role against various diseases and aging processes. The study objective was to investigate phytochemical screenings and antioxidant activity of ethanol fractions Euchema spinosum from south Bangka waters. Antioxidant activities of fresh algae (FA), dry algae (DA), and algae product (AP) were determined with DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Extraction was conducted using 70% ethanol in acidic environment for 24 hours. Then, the extract was evaporated using rotary evaporator. Dry extract was fractionated with ethanol - n - hexane ( 2 : 3 ) and the ethanol fraction was dried. The phytochemical screenings showed that all fractions had flavonoid, alkaloid and triterpenoid compounds. The most potent antioxidant activity was found in the ethanol fraction of AP with IC50 value 333.66 μg/mL, FA 418.32 μg/mL and DA 472.14 μg/mL. Based on the results, it can be concluded that the ethanol fraction of algae product can be used as a antioxidant source.

Bahasa Abstract

Alga merah (Eucheuma spinosum) dari Perairan Bangka Selatan mengandung pigmen merah yang bersifat sebagai antioksidan. Antioksidan alami dari alga diketahui berperan penting dalam melawan berbagai penyakit dan proses penuaan. Penelitian ini bertujuan untuk menentukan skrining fitokimia dan aktivitas antioksidan fraksi etanol alga Euchema spinosum dari Perairan Bangka Selatan. Alga dalam keadaan segar (AS), alga kering (AK) dan alga olahan (AO) ditentukan aktivitas antioksidannya menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Ekstraksi menggunakan etanol 70% dalam suasana asam selama 24 jam dan diuapkan dengan rotary evaporator. Ekstrak kering difraksinasi dengan etanol-n-heksana (2:3) dan fraksi etanol dikeringkan. Skrining fitokimia menunjukkan semua fraksi mengandung senyawa flavonoid, alkaloid dan triterpenoid. Aktivitas antioksidan paling kuat ditemukan pada fraksi etanol AO dengan nilai IC50 333,66 μg/mL, AS 418,32 μg/mL dan AK 472,14 μg/mL. Berdasarkan hasil tersebut, dapat disimpulkan bahwa fraksi etanol alga olahan dapat dimanfaatkan sebagai sumber antioksidan alami.

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Recommended Citation

Sari, Bina Lohita; Susanti, Nurulia; and Sutanto, Sutanto (2015) "Skrining Fitokimia dan Aktivitas Antioksidan Fraksi Etanol Alga Merah Eucheuma spinosum," Pharmaceutical Sciences and Research: Vol. 2: No. 2, Article 1.
DOI: 10.7454/psr.v2i1.3331
Available at: https://scholarhub.ui.ac.id/psr/vol2/iss2/1