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Abstract

Diabetes mellitus (DM) is a chronic metabolic disease that affects approximately 537 million people worldwide (1 in 10 individuals), and its prevalence is projected to reach 783 million by 2045. One of the plants that has the potential to be an antidiabetic is red shoots (Syzygium myrtifolium) since it contains various secondary metabolite compounds such as flavonoids, triterpenoids, steroids, saponins, phenolics, and anthocyanins. This study aimed to evaluate the antidiabetic properties of red shoot leaves extract and to identify and predict interactions between its active compounds and the α-glucosidase receptor. Ethanol, ethyl acetate, and n-hexane extracts of red shoot leaves were tested in vitro method for α-glucosidase inhibitory activity. The most active extract was analyzed using gas chromatography–mass spectrometry (GC–MS) to identify its chemical constituents. The identified compounds were then tested in silico using the molecular docking method to simulate interactions with the α-glucosidase receptor. The ethanol extract showed the strongest α-glucosidase inhibitory activity (IC₅₀ = 83.69 ppm), as compared to the ethyl acetate and n-hexane extracts. GC–MS analysis identified 5 secondary metabolites, predominantly terpenoids. Molecular docking revealed that three compounds includes α-Gurgujene, farnesyl acetate and Neophytadiene exhibited strong binding affinities (ΔG), hydrogen bonds and nteractions with the same amino acid residues as the standard control acarbose, indicating potential α-glucosidase inhibitory activity. These findings suggest that red shoot leaves extract, particularly its ethanol extract, contains bioactive compounds with promising potential as natural antidiabetic agents, warranting further pharmacological and clinical investigation.

References

Indonesian Food and Drug Authority. (2014). Regulation of the Head of the Indonesian Food and Drug Authority No. 12 of 2014 concerning Quality Requirements of Traditional Medicines. Jakarta: Head of Indonesian FDA 

Etsassala, N. G. E. R., Badmus, J. A., Marnewick, J. L., Egieyeh, S., Iwuoha, E. I., Nchu, F., & Hussein, A. A. (2022). Alpha-glucosidase and alpha-amylase inhibitory activities, molecular docking, and antioxidant capacities of Plectranthus colonic constituents. Antioxidants (Basel), 11(2), 378. https://doi.org/10.3390/antiox11020378 

Haryati, N. A., Saleh, C., & Erwin. (2016). Uji toksisitas dan aktivitas antibakteri ekstrak daun pucuk merah (Syzygium myrtifolium Walp.) terhadap Staphylococcus aureus dan Escherichia coli [Toxicity and antibacterial activity of red shoot (Syzygium myrtifolium Walp.) leaf extract against Staphylococcus aureus and Escherichia coli]. Jurnal Kimia Mulawarman, 13(1), 35–40. 

International Diabetes Federation. (2021). IDF Diabetes Atlas (10th ed.). Brussels: IDF. 

Jena, S., Ray, A., Sahoo, A., Das, P. K., Dash, K. T., Kar, S. K., & Nayak, S. (2021). Chemical composition and biological activities of leaf essential oil of Syzygium myrtifolium from Eastern India. Journal of Essential Oil-Bearing Plants, 24(3), 582–595. 

Juwita, R., Saleh, C., & Sitorus, S. (2017). Uji aktivitas antihiperurisemia daun pucuk merah (Syzygium myrtifolium Walp.) pada mencit jantan (Mus musculus) [Antihyperuricemic activity of red shoot (Syzygium myrtifolium Walp.) leaves in male mice (Mus musculus)]. Jurnal Atomik, 2(1), 162–168. 

Kumar, S., Narwal, S., Kumar, V., & Prakash, O. (2011). α-Glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacognosy Reviews, 5(9), 19–29. https://doi.org/10.4103/0973-7847.79096 

Mulyati, A. H., Alawiyah, H., Marom, M. F., & Warnasih, S. (2024). Antioxidant testing and identification of bioactive compounds in ethanol extract of propolis from various locations in Indonesia using LCMS-QTOF. Chimica et Natura Acta, 12, 193–203. 

Naufa, F., Mutiah, R., & Indrawijaya, Y. Y. A. (2022). Studi in silico potensi senyawa katekin teh hijau (Camellia sinensis) sebagai antivirus SARS-CoV-2 terhadap spike glycoprotein (6LZG) dan main protease (5R7Y) [In silico study of green tea (Camellia sinensis) catechins as SARS-CoV-2 inhibitors targeting spike glycoprotein (6LZG) and main protease (5R7Y)]. Journal of Food and Pharmaceutical Sciences, 10(1), 584–596. 

Obi, L. K., & Okwute, S. K. (2023). Chemical and biological investigations of the leaf extracts of Andrographis paniculata (Acanthaceae). Journal of Biomedical Research & Environmental Sciences, 4(3), 350–362. 

Patil, R., Das, S., Stanley, A., Yadav, L., Sudhakar, A., & Varma, A. K. (2010). Optimized Hydrophobic Interactions and Hydrogen Bonding at the Target-Ligand Interface Leads the Pathways of Drug-Designing. PLoS ONE, 5(8): e12029. 

Rasyid, A. (2016). Analisis metabolit sekunder, aktivitas antibakteri, dan komposisi senyawa dalam ekstrak teripang Bohadschia sp. [Secondary metabolites analysis, antibacterial activity, and compound composition of sea cucumber (Bohadschia sp.) extract]. Jurnal Ilmu dan Teknologi Kelautan Tropis, 8(2), 645–653. 

Reese, T. C., Devineni, A., Smith, T., Lalami, I., Ahn, J.-M., & Raj, G. V. (2024). Evaluating physicochemical properties of FDA-approved orally administered drugs. Expert Opinion on Drug Discovery, 19(2), 225–238. 

Suryati, Y., Taa, T. A., Fadhia, S. H., Ulia, R. V., Salsabilla, M. M., & Arifin, B. (2023). Komponen kimia minyak atsiri dari daun pucuk merah (Syzygium myrtifolium Walp.): Potensi antibakteri dan toksisitasnya [Chemical composition of essential oil from red shoot (Syzygium myrtifolium Walp.) leaves: Antibacterial potential and toxicity]. Jurnal Riset Kimia, 14(1), 70–80. 

Syafriana, V., Hamida, F., Damayanti, R., & Verananda, E. (2020). Aktivitas antibakteri ekstrak biji anggur (Vitis vinifera L.) terhadap Streptococcus pyogenes [Antibacterial activity of grape seed (Vitis vinifera L.) extract against Streptococcus pyogenes]. Sainstech Farma, 13(1), 40–44. 

Syarif, S., Nurnaningsih, & Pratama, M. (2020). Uji aktivitas ekstrak etanol daun kersen (Muntingia calabura L.) sebagai inhibitor enzim α-glukosidase dengan menggunakan Elisa Reader [Antidiabetic activity of ethanol extract of kersen (Muntingia calabura L.) leaves as α-glucosidase inhibitor using Elisa Reader]. Jurnal Fitofarmaka Indonesia, 7(2), 1–5. 

Hasti, S., Emrizal, & Susilawati, F. (2016). Antidiabetic activities test of n-hexane extract of Syzygium myrtifolium Walp. leaves on diabetic mice. Journal Pharmacy, 13(2), 172–181.

Tahya, C. Y., & Karnelasatri, K. (2021). Gas chromatography–mass spectrometry analysis and α-glucosidase inhibitory activity of n-hexane extract of Merremia vitifolia leaves. Walisongo Journal of Chemistry, 4(2), 162–172. 

Tiwari, R., Puthusseri, B., & Saha, S. (2019). β-Caryophyllene protects against diabetic cardiomyopathy through modulation of oxidative stress and inflammation. Journal of Molecular and Cellular Cardiology, 132, 191–201. 

Valdes, M., Calzada, F., & Mendieta-Wejebe, J. (2019). Structure–activity relationship study of acyclic terpenes in blood glucose levels: Potential α-glucosidase and sodium–glucose cotransporter (SGLT-1) inhibitors. Molecules, 24(22), 4020.

 Widiastuti, D., Sinaga, S. E., Warnasih, S., Pujiyawati, E., Salam, S., & Putra, W. E. (2023). Identification of active compounds from Averrhoa bilimbi L. flower using LC–MS and evaluation of antidiabetic activity through in vitro and in silico approaches. Trends in Sciences, 20(8), 6761. 

Williamson, E. M. (2021). Synergy and other interactions in phytomedicines. Phytomedicine, 85, 153497. https://doi.org/10.1016/j.phymed.2021.153497 

Windardi, I. (2019). Komponen bioaktif, aktivitas antioksidan, dan inhibisi α-glukosidase in vitro daun gandaria untuk pencegahan hiperglikemia [Bioactive components, antioxidant activity, and α-glucosidase inhibition in vitro of gandaria leaves for hyperglycemia prevention]. Institut Pertanian Bogor. 

World Health Organization. (2019). Classification of diabetes mellitus. Geneva: World Health Organization.

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