Abstract
Gummy candies have emerged as one of the most popular dosage forms for multivitamins and nutraceutical supplements, including those for brain health. Gelatin is the primary gelling agent used to achieve the gummy texture; however, the influence of different gelatin sources on the physicochemical properties of gummies has not been systematically examined. This study investigated the physicochemical characteristics of gummies prepared with porcine, bovine, and fish gelatin. The gummies were evaluated for mechanical properties (gumminess, hardness, cohesiveness, springiness, adhesiveness, and stringiness), color, water activity, antioxidant activity, and protein content. Additionally, the effects of intentional adulteration—specifically the addition of porcine gelatin to fish or bovine gelatin—were assessed. The results showed that gumminess, hardness, cohesiveness, and springiness were comparable across different gelatin sources, whereas adhesiveness and stringiness were highly sensitive to source variation. Notably, adulteration with porcine gelatin significantly altered those two parameters. These findings underscored the importance of verifying gelatin origin in gummy formulations, as source-related variations and gelatin adulteration could have a direct impact on product quality and performance.
References
Abd, M., Saeed, E., Mirghani, M. E. S., & Adam, A. (2005). Fish gelatin and its applications in selected pharmaceutical aspects as alternative source to pork gelatin. Journal of Food, Agriculture and Environmental, 11(1), 73–79. https://www.researchgate.net/publication/312332853
Afjani, Q., Baiti, N., Novrizal, M., Sahid, A., Darsih, C., & Laksitorini, M. D. (2024). The development of plant-based jelly candy for Chrysanthemum indicum L. flower extract and evaluation of the antioxidant activity. Indonesian Journal of Pharmacy, 35(2), 292–304.
Alemán, A., Giménez, B., Montero, P., & Gómez-Guillén, M. C. (2011). Antioxidant activity of several marine skin gelatins. LWT, 44(2), 407–413. https://doi.org/10.1016/j.lwt.2010.09.003
Alipal, J., Mohd Pu’ad, N. A. S., Lee, T. C., Nayan, N. H. M., Sahari, N., Basri, H., Idris, M. I., & Abdullah, H. Z. (2019). A review of gelatin: Properties, sources, process, applications, and commercialisation. Materials Today: Proceedings, 42, 240–250. https://doi.org/10.1016/j.matpr.2020.12.922
Aurelia, J., Trifena, F., Levi, K., Marcella, M., Septiyani, & Agustinah, W. (2022). Dragon Fruit peel extract and encapsulated catfish oil formulation in gummy candy with potential in vitro antihyperglycemia properties. Journal of Functional Food and Nutraceutical, 71–81. https://doi.org/10.33555/jffn.v3i2.87
Beuchat, L. R., Komitopoulou, E., Beckers, H., Betts, R. P., Bourdichon, F., Fanning, S., Joosten, H. M., & Kuile, B. H. T. (2013). Low-water activity foods: Increased concern as vehicles of foodborne pathogens. Journal of Food Protection, 76(1), 150–172. https://doi.org/10.4315/0362-028X.JFP-12-211
Devi, D. N., Darsih, C., Yuniarti, N., Ardiningtyas, B., & Laksitorini, M. D. (2024). Formulation and antioxidant activity of gotu kola jelly candy with plant-based polymers as a gelling agent. Majalah Obat Tradisional (Trad. Med. J.), 29(3), 266–275. https://doi.org/10.22146/mot.89699
Gomez-Guillen, M. C., Gimenez, B., Lopez-Caballero, M. E., & Montero, M. P. (2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A review. In Food Hydrocolloids (Vol. 25, Issue 8, pp. 1813–1827). Elsevier B.V. https://doi.org/10.1016/j.foodhyd.2011.02.007
Li, Z., & Srigley, C. T. (2017). A novel method for the quantification of long-chain omega-3 polyunsaturated fatty acids (PUFA) in gummy dietary supplements. Journal of Food Composition and Analysis, 56, 1–10. https://doi.org/10.1016/j.jfca.2016.11.006
Marfil, P. H. M., Anhê, A. C. B. M., & Telis, V. R. N. (2012). Texture and microstructure of gelatin/corn starch-based gummy confections. Food Biophysics, 7(3), 236–243. https://doi.org/10.1007/s11483-012-9262-3
Ninan, G., Joseph, J., & Aliyamveettil, Z. A. (2014). A comparative study on the physical, chemical and functional properties of carp skin and mammalian gelatins. Journal of Food Science and Technology, 51(9), 2085–2091. https://doi.org/10.1007/s13197-012-0681-4
Nur Hanani, Z. A., Roos, Y. H., & Kerry, J. P. (2012). Use of beef, pork and fish gelatin sources in the manufacture of films and assessment of their composition and mechanical properties. Food Hydrocolloids, 29(1), 144–151. https://doi.org/10.1016/j.foodhyd.2012.01.015
Otsuki, K., Uchino, S., Li, D., Kikuchi, T., Tei, K., & Li, W. (2024). Antioxidant activity of gelatins from sika deer (Cervus nippon). Pharmacognosy Research, 16(3), 588–593. https://doi.org/10.5530/pres.16.3.70
Rahmasari, F. S., Sahid, M. N. A., Siswanti, D. U., Darsih, C., Utami, I. D., Alam, L. P. M., & Laksitorini, M. D. (2024). The Development of antioxidant nutraceuticals containing chrysanthemum indicum l. gummy candy. Majalah Obat Tradisional, 29(1), 37–45. https://doi.org/10.22146/mot.87112
Ryveka, A., Lestari, L., Pratiwi, D., & Sundjaya, T. (2023). The development of multivitamin mineral jelly candy “previmin” for stunting prevention. Amerta Nutrition, 7(3SP). https://doi.org/https://doi.org/10.20473/amnt.v7i3SP.2023.10-19
Saha, T., Rifat, T., Shimanto, S. (2019). Prospects of halal pharmaceuticals. Asian Journal of Ethnopharmacology and Medical Foods, 5(2), 17–23.
Salamah, N., Erwanto, Y., Martono, S., & Rohman, A. (2021). The employment of real-time polymerase chain reaction using species-specific primer targeting on d-loop mitochondria for identification of porcine gelatin in soft candy. Indonesian Journal of Chemistry, 21(4), 852–859. https://doi.org/10.22146/ijc.60413
Salsabila, Z., Hardyawati, W., Ramadhan, M. B., Karen, E., Valencia, V., Yuniarti, N., Murwanti, R., & Laksitorini, M. (2025). Incorporation of cod liver oil to Centella asiatica (gotu kola) gummy candy as a cognitive enhancement supplement. Journal of Research in Pharmacy, 29(3), 1067–1077. https://doi.org/10.12991/jrespharm.1694222
Sam, T. (2000). Regulatory implications of excipient changes in medicinal products. Drug Information Journal, 34, 875–894.
Shukla, G., Candramauli, & Balawamy. (2024). Happi gummies L-carnitine L-trtrate:A comprehensive nutritive gummy formula for energy, brain and heart health support. International Journal of Pharmaceuticals and Health Care Research, 12, 55–3.
Syaifabila, I., Yuniarti, N., Darsih, C., & Laksitorini, M. D. (2024). Development of gotu kola (Centella asiatica (L.) Urban) gummy candy and its evaluation on antioxidant activity. Journal of Research in Pharmacy, 28(4)(28(4)), 1047–1056. https://doi.org/10.29228/jrp.787
Tarahi, M., Mohamadzade Fakhr-davood, M., Ghaedrahmati, S., Roshanak, S., & Shahidi, F. (2023). Physicochemical and sensory properties of vegan gummy candies enriched with high-fiber jaban watermelon exocarp powder. Foods, 12(7). https://doi.org/10.3390/foods12071478
Tarahi, M., Tahmouzi, S., Kianiani, M. R., Ezzati, S., Hedayati, S., & Niakousari, M. (2024). Current innovations in the development of functional gummy candies. In Foods (Vol. 13, Issue 1). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/foods13010076
Vojvodić Cebin, A., Bunić, M., Mandura Jarić, A., Šeremet, D., & Komes, D. (2024). Physicochemical and sensory stability evaluation of gummy candies fortified with mountain germander extract and prebiotics. Polymers, 16(2). https://doi.org/10.3390/polym16020259
Recommended Citation
Laksitorini, Marlyn Dian; Darsih, Cici; Yuniarti, Nunung; Rumiyati, Rumiyati; and Abidin, Mohammad Zainal
(2026)
"Comparative Physicochemical Characterization of Porcine, Fish, and Bovine Gelatin in Gummy Candies: Toward Halal Nutraceutical Development,"
Pharmaceutical Sciences and Research: Vol. 12:
No.
3, Article 1.
DOI: 10.7454/psr.v12i3.1448
Available at:
https://scholarhub.ui.ac.id/psr/vol12/iss3/1
Included in
Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacy and Pharmaceutical Sciences Commons, Pharmaceutics and Drug Design Commons


