Abstract
Kaffir lime peel waste (Citrus hystrix) has antioxidant effects that have the potential to protect the skin from exposure to UV-A and UV-B rays, as well as moisturize the skin. This study aims to determine the effect of increasing the concentration of condensed extract of kaffir lime fruit peel on its effectiveness as an antioxidant, sunscreen activity, and moisturizer. The condensed extract was obtained by maceration using 96% ethanol. In this study, we tested hydrophilic gels consisting of 4 extract concentrations in their formula: F1 (7.5%), F2 (10%), F3 (15%), and F4 (20%). The research phase begins with the extract standardization process and gel formulation, followed by testing physical quality and efficacy as an antioxidant, sunscreen, and moisturizing activity. Experimental data from different batches and formulas were analyzed using the One-Way ANOVA statistical method. The condensed extract (20%) provides an IC50 value of 15.51 mg/mL. The results of the experiment showed that increasing the concentration of kaffir lime fruit peel extract could reduce the pH value, viscosity and increase the spreadability of the gel preparation. The SPF value increased with increasing extract concentration. It is included in the maximum protection category, while based on the % Erythema Transmission Value ≤ 1 and % Transmission Pigmentation of 3-40, it falls under the total block category. Increasing the concentration of a condensed extract of kaffir lime fruit peel in a hydrophilic gel base can increase its effectiveness as a UV protector and a moisturizer, with F3 (15%) as the best formula.
References
Abdullah A, Haron H, Yusof H, Camalxaman SN, Mohamed E, & Rambely AZ. (2023). Antioxidant potential and phytochemical content from peels of three different citrus species: A mini review. Journal of Sustainability Science and Management, 18(12), 123–135. https://doi.org/10.46754/jssm.2023.12.011
Bayan, MF., Chandrasekaran, B & Alyami , MH. (2023). Development and characterization of econazole topical gel, Gels. 9, 929. https://doi.org/10.3390/gels9120929
Budaraga, I. K & Putra, D.P. (2021). Analysis antioxidant IC50 liquid smoke of cocoa skin with several purification methods. IOP Conference Series: Earth and Environmental Science, 757(1). https://doi.org/10.1088/1755-1315/757/1/012053
Chaudhary, P., Janmeda, P., Docea, AO., Yeskaliyeva, B., Razis, AFA., Modu, B., Calina, D., & Sharifi-Rad, J. (2023). Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases, Available at: 10.3389/fchem.2023.1158198, Frontiers in Chemistry, 10.3389/fchem.2023.1158198
Darsono, Soegianto, & Jesica. (2022). Study efektivitas ekstrak kental kulit buah jeruk purut terstandar (Citrus hystrix) sebagai antioksidan dan antijerawat. Pharmaceutical Journal of Indonesia. https://doi.org/10.21776/ub.pji.2022.008.01.6
Darsono, Soegianto & Jesica (2024). Development of standardized kaffir lime fruit peel extract as a gel for antioxidant and anti-acne. Pharmaciana, 14(1), 57. https://doi.org/10.12928/pharmaciana.v14i1.27076
DirJen POM. (2000). Parameter Standar Umum Ekstrak Tanaman Obat. Departemen Kesehatan RI. 2000: pp. 10–11
Elena, O.B., Maria, N.A., Michael, S.Z., Natalia, B.D., Alexander, I.B., & Ivan, I. K. (2022). Dermatologic gels spreadability measuring methods comparative study. International Journal of Applied Pharmaceutics, 14(1),164–168.https://doi.org/10.22159/ijap.2022v14i1.41267
Fitriansyah, S.N., Fadhilah, S, Ruslan, K., Hartati, R., Fidrianny, I. (2023). Antioxidant activity and sun protection factor of different parts of sawo walanda (Pouteria campechiana ( Kunth .) B .) extract. Indonesian Journal of Pharmaceutical Science and Technology, SUPP 5(2), 207-214
Gabros, S., Nessel, T.A., & Zito, P.M. (2023). Sunscreens and Photoprotection. StatPearls Publishing. 2023. https://www.ncbi.nlm.nih.gov/ books/ NBK537164/?report=printable
Gotwals, J., Stump-Sutli, K., & Lehrer, M. (2021). Ultraviolet (UV) radiation and your health types of UV rays how uv rays affect your health what is the UV Index ?. pp. 23–26. https://www.urmc.rochester.edu/encyclopedia /content.aspx?contenttypeid =85&contentid=P01359
Gromkowska-Kępka, K. J., Puścion-Jakubik, A., Markiewicz-Żukowska, R., & Socha, K. (2021). The impact of ultraviolet radiation on skin photoaging - review of in vitro studies. Journal of Cosmetic Dermatology, 20(11), 3427–3431. https://doi.org/10.1111/jocd.14033
Herranz-Lopez & Barrajon-Catalan (2020). Antioxidants and skin protection. Antioxidants, 9(8), 1–4. https://doi. org/10.3390/antiox9080704
Herrero-Fernandez, M., Montero-Vilchez, T., Diaz-Calvillo, P., Romera-Vilchez, M., Buendia-Eisman, A., & Arias-Santiago, S. (2022). Impact of water exposure and temperature changes on skin barrier function. Journal of Clinical Medicine, 11(2), 298. https://doi.org/10.3390/jcm11020298
Indonesian FDA. (2020). Peraturan Badan Pengawas Obat dan Makanan Nomor 30 Tahun 2020 tentang Persyaratan Teknis Penggunaan Kosmetika. BPOM RI, 1–16.
Jesus A, Mota S, Torres A, Cruz, MT, Sousa E, Almeida IF, & Cidade H. (2023). Antioxidants in Sunscreens: Which and What For?, Antioxidants. 2023; 12: 138. https://doi.org/10.3390/antiox12010138
Kemenkes RI (2022). Suplemen I Farmakope Herbal Indonesia Edisi II. Jakarta: Departement Kesehatan Republik Indonesia.
Knox S, & O’Boyle NM. (2021). Skin lipids in health and disease: A review. Chemistry and Physics of Lipids, 236(January), 105055. https://doi.org/10.1016/j.chemphyslip.2021.105055
Levy, SB. (2023). In vitro antibacterial and antioxidant activities of roasted and green coffee beans originating from different regions of Ethiopia. International Journal of Food Science, 2020. https://doi.org/10.1155/2020/8490492
Mawazi SM, Ann J, Othman N, Khan J, Alolayan SO, Al thagfan SS, & Kaleemullah M. (2022). A Review of Moisturizers; History, Preparation, Characterization and Applications. Cosmetics, 9, 61. https://doi.org/10.3390/cosmetics9030061
Michalak, M., Pierzak, M., Kręcisz, B., & Suliga, E. (2021). Bioactive compounds for skin health: A review. Nutrients, 13(1), 203. https://doi.org/10.3390/nu13010203
Mukherjee, A. (2021). The Good, Bad and Bubbly Of Surfactants In Skincare – SkinKraft, 1–9. Retrieved from https://skinkraft.com/blogs/articles/role-of-surfactants-in-skincare, SkinKraft Laboratory.
Naik SN, & Desai S. (2019). Estimation and comparison of hydro- alcoholic and water extract for sun protection factor activity from naturally available resources. International Journal of Environmental Sciences & Natural Resources, 21(1). https://doi.org/10.19080/ ijesnr.2019.21.556052
Ngoc LTN, Tran VV, Moon JY, Chae M, Park D, & Lee YC. (2019). Recent trends of sunscreen cosmetic: An update review. Cosmetics. 2019: 6(4):64. https://doi.org/10.3390 /cosmetics6040064,
Nur S, Hanafi M, Setiawan H, & Elya B. (2022). In vitro ultra violet (UV) protection of curculigo latifolia extract as a sunscreen candidate. IOP Conference Series: Earth and Environmental Science, 1116(1). https://doi.org/10.1088/1755-1315/1116/1/012009
Portilho, L., Aiello, L.M., Vasques, L.I., Bagatin, E., & Leonardi, G.R. (2022). Effectiveness of sunscreens and factors influencing sun protection: a review. Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e20693
Pyrzynska, K. (2022). Hesperidin: A Review on Extraction Methods, Stability and Biological Activities. Nutrients, 14(12), 2387. https://doi.org/10.3390/nu14122387
Rajkumar, J., Chandan, N., Lio, P., & Shi, V. (2023). The skin barrier and moisturization: Function, disruption, and mechanisms of repair. Skin Pharmacology and Physiology, 36(4), 174–185. https://doi.org/10.1159/000534136
Rijar, GY., Sari, N., & Aliah, AI. (2022). Comparison of percent value of erythema and pigmentation transmission with maceration and infusion method of Robusta Coffee (Coffea canephora Pierre A. Frohner) derived from the district Tana Toraja. Jurnal Multidisiplin Madani (MUDIMA), 2(6), https://doi.org/10.55927/mudima.v2i6.443
Riski, R., Nur, S., Pabontong, E. (2021). Activity test and formulation of antioxidant cream from ethanol extract combination of several citrus fruit peels (Citrus sp.). Journal of Pharmaceutical and Medicinal Sciences.
Rodrigues, C. V., & Pintado, M. (2024). Hesperidin from orange peel as a promising skincare bioactive: An overview. International Journal of Molecular Sciences, 25(3), 1890. https://doi.org/10.3390/ijms25031890
Saffrin, M.A., & Sureshkumar, R. (2019). An overview on herbal sunscreen formulation and sun protection factor value. Journal of Pharmaceutical and Scientific Innovation, 8(4), 127–135. https://doi.org/10.7897/2277-4572.084141
Safitri, FI., Nawangsari, D & Febrina, D. (2020). Overview: Application of Carbopol 940 in Gel. Advances in Health Sciences Research, volume 34, Proceedings of the International Conference on Health and Medical Sciences (AHMS 2020), Published by Atlantis Press B.V.DOI: 10.2991/ahsr.k.210127.018
Salomon, G., & Giordano-Labadie, F. (2022). Surfactant irritations and allergies. Surfactant irritations and allergies. European Journal of Dermatology : EJD, 32(6), 677–681. https://doi.org/10.1684/ejd.2022.4290
Shah, Y., & Mewada, W. (2023). Recent advances in sunscreen agents and their formulations: A review. International Journal of Pharmaceutical Chemistry and Analysis, 9(4):141–150, https://doi.org/10.18231/j.ijpca.2022.027
Shilpa, VS., Shams, R., Dash, KK., Pandey, VK., Dar, AH., Mukarram, SA., Harsányi, ., & Kovács, B. (2023). Phytochemical properties, extraction, and pharmacological benefits of naringin: A review. Molecules. 28, 5623. https://doi.org/10.3390/molecules28155623
Siti, H. N., Mohamed, S., & Kamisah, Y. (2022). Potential therapeutic effects of Citrus hystrix DC and its bioactive compounds on metabolic disorders. Pharmaceuticals (Basel, Switzerland), 15(2), 167. https://doi.org/10.3390/ph15020167
SNI. (1996). Sediaan Tabir Surya,Jakarta:Dewan Standarisasi Nasional. 1996.
Solano, F. (2020). Photoprotection and skin pigmentation: Melanin-related molecules and some other new agents obtained from natural sources. Molecules, 25(7), 1–18. https://doi.org/10.3390/molecules25071537
Sonkar, P.K., & Kumar. (2022). pH Determination of Water Sample Using A pH Meter, In: Laboratory Manual for School projects. Publisher: Aknik Publication. India. 2020:p.50-53
Strodhal, D. (2023). Paula’s Choice Skin Care Antioxidant for Better Sun Protection. https://www.paulaschoice.com/expert-advice/skincare-advice/ingredient-spotlight/antioxidants-sunscreen
Su, CY., Morocho-Jácome, AL., Lima,FB., Marques, GA., Rodriguez, WLA., Julca, CAC., Robles, ERA., Rosado, C., Velasco, MVR., & Baby, AR. (2020). In vitro water resistance evaluation of a bioactive sunscreen containing distinct film /barrier-forming agents. Biomedical and Biopharmaceutical Research, 17(2): 313-326
Susanto, D.W.C., Yamlean, P.V.Y,, & Mansauda, K.R.L. (2024). Formulasi dan evaluasi krim kombinasi ekstrak kulit semangka (Citrullus lanatus) dan ekstrak kulit pepaya (Carica papaya L.) sebagai pelembap. Pharmacon, 13(1). DOI: 10.35799/pha.13.2024.49564
Verma, A., Zanoletti, A., Kareem, KY., Adelodun, B., Kumar, P., Ajibade, FO., Silva, LFO., Phillips, AJ., Kartheeswaran, T., Bontempi, T., & Dwivedi, A. (2023). Skin protection from solar ultraviolet radiation using natural compounds: A review. Environmental Chemistry Letters, https://doi.org/10.1007/s10311-023-01649-4
Wahyudi, R., Kasjono, HS., & Haryanti, S. (2022). Efektivitas penurunan surfaktan pada air limbah cuci tangan menggunakan filtrasi kelara, Jurnal Ruwa Jurai, 16(3), 146-151. http://dx.doi.org/10.26630/rj.v16i3.3588
Yusharyahya, S,N. (2021). Mekanisme penuaan kulit sebagai dasar pencegahan dan pengobatan kulit menua. e-Journal Kedokteran Indonesia, 9(2),150. https://doi.org/10.23886/ejki.9.49.150
Zaim, R., Wahab, M., Ismail, H.F., Othman, N., & Hara, H. (2023). Extraction and determination of flavonoid compounds in citrus fruit waste. IOP Conf. Series: Earth and Environmental Science. 7th Malaysia-Japan Joint International Conference. 1144 012005. https//www.doi:10.1088/1755-1315/1144/1/012005
Zou, W., Ramanathan, R., Urban, S., Sinclair, C., King, K., Tinker, R., & Bansal, V. (2022). Sunscreen testing: A critical perspective and future roadmap. TrAC - Trends in Analytical Chemistry, 157, 116724. https://doi.org/10.1016/j.trac.2022.116724
Zhou L, Peng Y, Xu Z, Chen J, Zhang N, Liang T, Chen T, Xiao Y, Feng S. & Ding C. (2024). The antioxidant, anti-inflammatory and moisturizing effects of Camellia oleifera oil and its potential applications. Molecules. 2024;29:1864. Available at: https://doi.org/10.3390/molecules29081864
Recommended Citation
Darsono, Farida Lanawati and Soegianto, Lisa
(2025)
"Formulation and In vitro Evaluation of Kaffir Lime Fruit Peel Extract-Containing Gel for Sunscreen, Antioxidant, and Moisturizing Activity,"
Pharmaceutical Sciences and Research: Vol. 12:
No.
2, Article 1.
DOI: 10.7454/psr.v12i2.1424
Available at:
https://scholarhub.ui.ac.id/psr/vol12/iss2/1
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