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Abstract

Indonesia is rich in aromatic plants, producing approximately 40 types of essential oils used in various industries, including health and aromatherapy. Essential oils have significant potential but face high rates of adulteration. This study aimed to identify kaffir lime leaf oil (Citrus hystrix D.C.), to determine the essential oil profile, and to detect adulteration in commercial oil products. The methods used involved steam distillation and characterization tests of kaffir lime leaf essential oil, including yield percentage and organoleptic, density, refractive index, and solubility in alcohol. An essential oil content test and adulteration detection using FTIR combined with chemometrics were also conducted. The FTIR results showed distinctive peaks for kaffir lime leaf oil, allowing the identification of the main compounds in the oil. Multivariate analysis using the PLS model could predict oil adulteration with RMSEC 1.319, RMSECV 1.913, and RMSEP 2.362, indicating the model’s accuracy. Furthermore, PCA successfully separated and grouped the tested oils. This study concludes that the analyzed oil was kaffir lime leaf oil, based on the characterization results and FTIR spectra showing distinctive peaks of the oil. PLS and PCA analysis showed that the three tested commercial oil products did not contain turpentine adulterants.

References

Agatonovic-Kustrin, S., Ristivojevic, P., Gegechkori, V., Litvinova, T. M., & Morton, D. W. (2020). Essential oil quality and purity evaluation via ft-ir spectroscopy and pattern recognition techniques. Applied Sciences (Switzerland), 10(20), 1–12. https://doi.org/10.3390/app10207294

Baptista-Silva, S., Borges, S., Ramos, O. L., Pintado, M., & Sarmento, B. (2020). The progress of essential oils as potential therapeutic agents: A review. Journal of Essential Oil Research, 32(4), 279-295

BSN. (2014). Essential oil distillation apparatus — Part 1: Steam system — Specifications and test methods (SNI 8028-1:2014). Jakarta: Badan Standardisasi Nasional.

BSN. (2023). Essential oil of kaffir lime leaves (Citrus hystrix DC.) — Specification (SNI 9231:2023). Jakarta: Badan Standardisasi Nasional.

Cui, G., Liu, Z., Wei, M., & Yang, L. (2018). Turpentine as an alternative solvent for the extraction of gutta-percha from Eucommia ulmoides barks. Industrial Crops and Products, 121, 142-150.

Dhavesia, V. (2017). Uji aktivitas antibakteri ekstrak daun jeruk purut (Citrus hystrix D. C.) terhadap Pseudomonas aeruginosa dan Staphylococcus epidermidis. Universitas Atma Jaya Yogyakarta.

Febrina, D. (2019). Pengaruh perbedaan perlakuan pendahuluan terhadap rendemen minyak atsiri daun jeruk purut (Citrus hystrix DC). Viva Medika, 11. http://ejournal.uhb.ac.id/index.php/VM/issue/archive

Guntarti, A., Nurani, L. H., Lestari, P., Edityaningrum, C. A., Irham, L. M., & Rohman, A. (2024). Authentication of clove leaf oil in products (Syzygium aromaticum (L.) Merr. & Lm Perry) using GC-MS and FTIR methods combined with chemometric. Malaysian Journal of Analytical Sciences, 28(3), 664-680

Guntarti, A., Salamah, N., & Fadli, J. C. (2025). Authentication of commercial corn oil products using combined GC-MS and FTIR chemometric methods. Tropical Journal of Natural Product Research, 9(4).

Hussain, S. Z., & Maqbool, K. (2014). GC-MS: Principle, technique and its application in food science. International Journal of Current Science Research and Review, 13, 116–126.

Kawiji, Khasanah, L. U., & Aryani, N. T. (2015). Ekstraksi maserasi oleoresin daun jeruk purut (Citrus hystrix DC): Optimasi rendemen dan pengujian karakteristik mutu. AGRITECH, 35(2), 178–184.

Kemendag RI. (2021). Indonesian Essential Oil The Scents of Natural Life 1st Edition. Ministry of Trade Republic of Indonesia.

Latifah, F., Taufiq, H., & Fitriyana, N. M. (2023). Uji antioksidan dan karakterisasi minyak atsiri dari kulit jeruk purut (Citrus hystrix D. C). Journal of Pharmaceutical Science and Clinical Research, 8(1), 46–62. https://doi.org/10.20961/jpscr.v8i1.67396

Mahendra, M. R., Salamah, N., & Guntarti, A. (2024). Purity analysis of sesame oil extracted from Sesamum indicum L. using the ATR-FTIR method. Indonesian Journal of Pharmaceutical Science and Technology, 11(3), pp. 382-392.

Maulida, Z. (2019). Pengaruh perlakuan pendahuluan fermentasi terhadap kualitas minyak atsiri kulit jeruk purut (Citrus hystrix). Fakultas Pertanian Universitas Brawijaya.

Miftahendarwati. (2014). Efek Antibakteri Ekstrak Daun Jeruk Purut (Citrus hystrix) terhadap Bakteri Streptococcus mutans (in vitro). Universitas Hasanuddin.

Miller, J. N., & Miller, J. C. (2010). Statistics and Chemometrics for Analytical Chemistry Sixth edition. Pearson Education Limited. www.pearsoned.co.uk/Miller

Pavia, D. L., Lampman, G. M., & Kriz, G. S. (2010). Introduction to Spectroscopy 3rd Edition. Thomson Learning. https://doi.org/10.1520/stp37187s

Purwakusumah, E. D., Rafi, M., Syafitri, U. D., Nurcholis, W., & Adzkiya, M. A. Z. (2014). Identifikasi dan autentikasi jahe merah menggunakan kombinasi spektroskopi FTIR dan Kemometrik. AGRITECH, 34(1), 82-87.

Rafi, M., Anggundari, W. C., & Irawadi, T. T. (2016). Potensi spektroskopi FT-IR-ATR dan kemometrik untuk membedakan rambut babi, kambing dan sapi. Indonesian Journal of Chemical Science, 5(3), 229-234. http://journal.unnes.ac.id/sju/index.php/ijcs

Rifqi, A., Siadi, K., & Sudarmin. (2014). Isolasi sitronelal dari minyak sereh dan oksidasinya dengan KMnO4 dalam suasana basa. Indonesian Journal of Chemical Science, 3(3), 198-202.

Rohman, A., Irnawati, & Riswanto, F. D. O. (2020). Kemometrika. Gadjah Mada University Press.

Rohman, A., Windarsih, A., Riyanto, S., Sudjadi, Shuhel Ahmad, S. A., Rosman, A. S., & Yusoff, F. Mohd. (2016). Fourier transform infrared spectroscopy combined with multivariate calibrations for the authentication of avocado oil. International Journal of Food Properties, 19(3), 680–687. https://doi.org/10.1080/10942912.2015.1039029

Salamah, N., Cantika, C. D., Nurani, L.H., & Guntarti. A. (2024). Authentication of citrus peel oils from different species and commercial products using FTIR Spectroscopy combined with chemometrics. Pharmacia, 71(1), pp. 107

Smith, M. B., & March, J. (2006). March’s Advanced Organic Chemistry: Reactions, Mechanisms, and Structure 6th Edition. John Wiley & Sons. https://doi.org/10.1002/0470084960

Sopi, N. Z. M., Hamzah, M. H., Man, H. C., Nor, M. Z. M., & Radzuan, M. N. (2021). Process optimization of ultrasonic-assisted-soxhlet-extraction of essential oil from Citrus hystrix using response surface methodology. AMA, 52(3), 4043–4061.

Syafri, S., Jaswir, I., Yusof, F., Rohman, A., Ahda, M., & Hamidi, D. (2022). The use of instrumental technique and chemometrics for essential oil authentication: A review. Results in Chemistry, 4, 100622.

Yanti, R., Wulandari, P., Pranoto, Y., & Cahyanto, M. N. (2017). Karakterisasi, identifikasi dan uji aktivitas anti jamur minyak atsiri daun jeruk purut (Citrus hystrix) terhadap aspergillus. Jurnal Teknologi Pertanian, 8(2), 8–15.

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