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Abstract

Acne can be caused by Staphylococcus epidermidis and Propionibacterium acnes. By using antibiotic for the acne theraphy in a long term period can cause resistance, organ detriment and imunohypersensitivity. Red dragon fruit’s peel (Hylocereus polyrhizus) is one of natural materials which can be used for alternative antibacterial. This study aims to determine the antibacterial activity of n-Hexane fraction of red dragon fruit’s peel against S. epidermidis dan P. acnes by the method of disc diffusion Kirby-Bauer. Red dragon fruit’s peel powder macerated within chloroform, and macerate then fractionated with n-Hexane. Phytochemical screening result red dragon fruit’s peel contains terpenoid and alkaloid. Clindamycin 4µg/disk were used for positive control while DMSO 10% were used for negative control. Data analyzed by using R-Commander program 3.0.3 version.This research show that n-Hexane fraction of red dragon fruit’s peel have antibacterial activity against P. acnes where the averagezone of inhibition obtained from the concentrations from 20; 40; and 80 mg/mL in a row were 9 mm; 10,25 mm; and 10,5 mm. In while, for S epidermidis n-hexane fraction of red dragon fruit’s peel didn’t have antibacterial activity.

Bahasa Abstract

Bakteri yang dapat memicu tumbuhnya jerawat diantaranya adalah Staphylococcus epidermidis dan Propionibacterium acnes. Pengobatan jerawat menggunakan antibiotik dalam jangka panjang dapat menimbulkan resistensi, kerusakan organ dan imuno hipersensitifitas. Kulit buah naga merah (Hylocereus polyrhizus) merupakan salah satu bahan alam yang dapat dimanfaatkan sebagai alternatif antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari fraksi n-Heksana kulit buah naga merah terhadap bakteri S. epidermidis dan P. acnes dengan metode disc diffusion Kirby-Bauer. Simplisia kulit buah naga merah dimaserasi dengan kloroform. Maserat yang didapat selanjutnya difraksinasi dengan n-Heksana. Hasil penapisan fitokimia fraksi n-Heksana kulit buah naga merah mengandung terpenoid dan alkaloid. Kontrol positif yang digunakan adalah klindamisin 4µg/disk sedangkan kontrol negatif yang digunakan adalah DMSO 10%. Analisis data menggunakan program R-Commander versi 3.0.3. Berdasarkan hasil penelitian fraksi n-Heksana kulit buah naga merah memiliki aktivitas antibakteri terhadap P. acnes, dengan rata-rata zona hambat dari konsentrasi 20; 40 dan 80 mg/mL masing-masing secara berurutan adalah 9 mm; 10,25 mm dan 10,5 mm. Sedangkan pada S. epidermidis fraksi n-Heksana kulit buah naga merah tidak memiliki aktivitas antibakteri.

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