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Abstract

Excessive free radical production will lead to oxidative stress. One of the indicators used to determine stress oxidative is malondialdehyde (MDA). Hylocereus polyhizus peel is potential used natural exogenous antioxidant. The aim of this research was to study the effect of n-hexane fraction of H.polyhizus peel on MDA level and to determine the dose that could reduce the MDA level. H.polyhizus peel was macerated with chloroform, fractionated with n-Hexane. Rats were divided into 7 groups : normal, negative, positive control vitamin E (18 mg/ kgbw), positive control quercetin (4 mg/200gbw), H.polyhizus peel extract group treated with dose I (10mg/ 200gbw), dose II (20 mg/200gbw) and dose III (40 mg/200gbw). Each group consist of 4 rats and induced to oxidative stress by 10 minute swimming each day and food restriction for 5 days. Measurement of MDA level are using Spectrophotometer UV-Vis in 532 nm. MDA levels were analyzed using One way Anova and LSD test. The data shown that MDA level at normal, negative, positive vitamin E, positive kuersetin, dose I, II, III group is 0.042; 0.051; 0.034; 0.042; 0.038; 0.039; 0.042 µg/ml. The n-Hexane fraction of red dragon fruit peel was a potent antioxidant because posessing the ability to lowering the MDA level on stress oxidative rats.

Bahasa Abstract

Produksi radikal bebas berlebihan dalam tubuh dapat menyebabkan stres oksidatif. Salah satu indikator stres oksidatif pada manusia adalah kadar Malondialdehida (MDA). Kulit buah Hylocereus polyrhizus berpotensi sebagai antioksidan eksogen alami. Tujuan penelitian ini untuk mengetahui efek fraksi n-Heksana kulit H.polyhizus terhadap kadar MDA dan mengetahui dosis fraksi n-Heksana H.polyhizus yang dapat menurunkan kadar MDA. Kulit H.polyhizus dimaserasi menggunakan kloroform p.a, kemudian difraksinasi dengan pelarut n-Heksana. Tikus dibagi menjadi 7 kelompok yaitu kontrol normal, kontrol negatif, kontrol positif vitamin E (18 mg/ kgBB), kontrol positif kuersetin (4 mg/200gBB), dosis I (10 mg/200gBB), Dosis II (20 mg/200gBB) dan dosis III (40 mg/ 200gBB). Setiap kelompok terdiri dari 4 ekor tikus dan diberikan stres oksidatif berupa perenangan 10 menit perhari dan puasa pakan selama 5 hari. Kadar MDA diukur menggunakan Spektrofotometer UV-Vis pada panjang gelombang 532 nm. Analisis data dilakukan dengan menggunakan One way ANOVA dan uji LSD (Least Significant Difference). Hasil analisis data kadar rata-rata MDA pada kelompok normal, negatif, positif vitamin E, positif kuersetin, dosis I, II dan III berturut-turut adalah 0,042; 0,051; 0,034; 0,042; 0,038; 0,039; 0,042 µg/ml. Dosis fraksi n-Heksana kulit H.polyhizus berpotensi sebagai antioksidan karena dapat menurunkan kadar MDA pada tikus yang mengalami stres oksidatif.

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