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Abstract

Production of excess free radicals cause oxidative damage and degenerative diseases. Catalase enzyme is one of the endogenous intracellular antioxidant that can be reduced by oxidative stress conditions. The peel of red dragon fruit (Hylocereus polyrhizus) as a potent exogenous antioxidants could support the internal enzyme activity to prevent free radicals. The aim of this research was to determine the effect of n-hexane fraction of H.polyrhizus’s peel and the dose that could increase activity of catalase. The peel was maserated with chloroform, then fractionated by n-hexane. Stress condition was achieved by five days fasting and swimming for 10 mins/day to test animals. UV-Vis spectrophotometer was used to measure the activity of catalase. The rats were divided into 6 groups: normal control, negative control, dose I (10 mg/200 gBB), dose II (40 mg/200 gBB), positive control quercetin as (4 mg/200 gBB) and vitamin E (17.64 mg/kg). The activity of catalase enzyme in the normal control is 29.4 units/ml; negative control is 7.08 units/ml; dose I is 14.42 units/ml; dose II is 14.79 units/ml’quercetin is 28.18 units/ml; and vitamin E is 71.29 units/ml. Our conclusion is the activity of the catalase enzyme rat under oxidative stress can be increased by the n-hexane fraction of H. polyrhizus.

Bahasa Abstract

Produksi radikal bebas berlebih dapat menyebabkan kerusakan oksidatif dan menimbulkan penyakit degeneratif. Enzim katalase yang merupakan salah satu enzim antioksidan endogen intrasel dapat mengalami penurunan aktivitas akibat kondisi stres oksidatif. Kulit buah naga merah (Hylocereus polyrhizus) berpotensi sebagai antioksidan eksogen yang dapat membantu kerja enzim antioksidan untuk mengatasi radikal bebas. Tujuan penelitian ini untuk mengetahui pengaruh pemberian fraksi n-heksana kulit buah H.polyrhizus dan dosis yang dapat meningkatkan aktivitas katalase. Simplisia kulit buah diisi dimaserasi dengan kloroform dan difraksinasi dengan n-heksana. Perlakuan stres dilakukan dengan puasa selama 5 hari dan berenang selama 10 menit/hari. Pengukuran aktivitas enzim katalase menggunakan spektrofotometer UV-Vis. Tikus dibagi menjadi 6 kelompok yaitu kontrol normal, kontrol negatif, perlakuan dosis I (10 mg/200 gBB), II (40 mg/200 gBB), kontrol postitif kuersetin (4 mg/200 gBB) dan vitamin E (17,64 mg/kgBB). Hasil analisis data aktivitas enzim katalase pada kelompok kontrol normal 29,4 Unit/ml, kontrol negatif 7,8 Unit/ml, dosis I 14,42 Unit/ml, dosis II 14,79 Unit/ml, kuersetin 28,18 Unit/ml, dan vitamin E 71,29 Unit/ml. Hasil penelitian menunjukkan bahwa, fraksi n-heksana H. polyrhizus berpotensi sebagai antioksidan karena dapat meningkatkan aktivitas enzim katalase tikus yang mengalami stres oksidatif.

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