To reduce the level of CO2 content in air, effort on converting CO2 to useful products is required. One of the alternatives includes CO2 fixation to produce biomass using Chlorella vulgaris Buitenzorg. Chlorella vulgaris Buitenzorg is applied for production of food supplement. Chlorella vulgaris Buitenzorg is also easy to handle due to its superior adaptation. Currently, Chlorella vulgaris Buitenzorg has been analyzed by some experts for its cellular composition, its ability to produce high quality biomass and the content of essential nutrition. A series of experiments was conducted by culturing Chlorella vulgaris Buitenzorg using Beneck medium in bubbling column photobioreactor. The main variation in this experiment was photoperiodicity, where growth of Chlorella vulgaris Buitenzorg was examined during photoperiodicity condition. The difference between CO2 gas concentration of inlet and outlet of the reactor during operational period, was compared to the same experiment under continuous illumination. Under photoperiodicity of 8 and 9 h/d, the culture cell densities (N) were approximately 40 % higher than under continuous illumination. Final biomass density of Chlorella vulgaris Buitenzorg at 9 h/d illumination was 1.43 g/dm3, around 46% higher than under continuous illumination. Specific carbon dioxide transfer rate (qCO2) in photoperiodicity was 50-80% higher than under continuous illumination. These experiments showed that photoperiodicity affects the growth of Chlorella vulgaris Buitenzorg The specific growth rate (μ) by photoperiodicity was higher than that by continuous ilumination while the growth period was two times longer. Based on the experiments, it can be concluded that photoperiodicity might save light energy consumption. The prediction of kinetic model under continuous illumination as well as under photoperiodicity illumination showed that Haldane model became the fitted kinetic model.


A. Wijanarko, K. Ohtaguchi, Comp. Biochem. and Physiol. 134 (2003) S214.

J. E. Bailey, D. F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill Book Co., New York, 1986.

S. Jacquet, F. Partensky, D. Marie, R. Casotti, D. Vaulot, Appl. Environ. Microbiol. 67 (2001) 782.

G. T. Marino, Y. Asato, J. Gen. Microbiol. 132 1986) 2123.

B. J. Binder, S. W. Chisholm, J. Bacteriol. 172 (1990) 2313.

A. F. Post, R. de Wit, L.R. Mur, J. of Plankton Res. 7 (1985) 487.

K. Ohtaguchi, S. Kajiwara, D. Mustaqim, N. Takahashi, Energy Convers. Mgt. 38 (1997) S523. [8] G. Oquist, J. M. Anderson, S. McCaffery, W. S. Chow, Planta 188 (1992) 422.

N. K. Boardman, Ann. Rev. Plant Physiol. 28 (1972) 355.

P. G. Falkowsky, T. G. Owens, Plant Physiol. 66 (1980) 592.

P. A. Ensminger, Life under the Sun, Yale University Press, London, 2001.

J.C. Ogbonna, H. Tanaka, J. of Ferment. Bioeng. 82 (1996) 558.

R. H. Foy, C. E. Gibson, R. V. Smith, J. Phycol. 11 (1976) 151.

N. Mann, N. G. Carr, J. Gen. Microbiol. 83 (1979) 399.

A. Kaplan, M. R. Badger, J. A. Berry, Planta 149 (1980) 219.

A. Wijanarko, K. Asami, K. Ohtaguchi, J. of Chem. Eng. Japan 37 (2004) 1019.

R. A. Pelroy, J. A. Bassham, Arch. Microbiol. 86 (1972)25.

K. Schugerl, K. H. Bellgardt, Bioreaction Engineering,, Modelling and Control, Springer-Verlag, Berlin, 2000.

A. Wijanarko, K. Ohtaguchi, Stud. Surf. Sci.Catal. 153 (2004) 461.

Cited By




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.