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Abstract

Hydroxyapatite (HAp) is one of the constituent minerals of bone and teeth, that has been widely used for synthesizing bone graft. Due to the limitation on properties of the hydroxyapatite, it is doping with Magnesium (HAp-Mg). The addition of Chitosan (Chi) was expected to improve the antibacterial properties of HAp-Mg. The present research aims to study the influence of Chitosan with 0, 5, 15, and 25 wt% addition on biocompatibility properties of Chi/HAp-Mg composite. HAp-Mg was synthesized using the sol-gel method; meanwhile, Chi/HAp-Mg composite was manufactured by mixing Chitosan in acetic acid, and HAp-Mg was added into the mixture. The synthesized samples were characterized using XRD and SEM. In vitro antibacterial activities of the Chi/HAp-Mg composite were evaluated against Escherichia coli bacteria. Biocompatibility analysis from antibacterial activity showed that composite with the optimal composition on the addition of 15 wt% Chitosan has the best ability in inhibiting the growth of E. coli bacteria.

Bahasa Abstract

Studi Antibakteri Komposit Kitosan/Hidroksiapatit Terdoping Magnesium sebagai Material untuk Aplikasi Cangkok Tulang. Hydroxyapatite (HAp) adalah salah satu mineral penyusun tulang dan gigi yang telah banyak digunakan untuk sintesis cangkok tulang. Karena keterbatasan pada sifat hidroksiapatit diperlukan doping dengan Magnesium (HAp-Mg). Penambahan Chitosan (Chi) diharapkan dapat meningkatkan sifat antibakteri dari HAp-Mg. Penelitian ini bertujuan untuk mempelajari pengaruh Chitosan dengan penambahan 0, 5, 15, dan 25 wt.% pada sifat biokompatibilitas komposit Chi/HAp-Mg. HAp-Mg disintesis menggunakan metode sol-gel; Sementara itu, komposit Chi/HAp-Mg diproduksi dengan mencampurkan Chitosan dalam asam asetat kemudian HAp-Mg ditambahkan ke dalam campuran. Sampel yang disintesis dikarakterisasi menggunakan XRD dan SEM. Aktivitas antibakteri in vitro dari komposit Chi/HAp-Mg diuji terhadap bakteri Escherichia coli. Analisis biokompatibilitas dari aktivitas antibakteri menunjukkan bahwa komposit dengan komposisi optimal pada penambahan 15 wt.% Chitosan memiliki kemampuan terbaik dalam menghambat pertumbuhan bakteri E. coli.

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