Component Analysis and Antiangiogenic Activity of Thailand Stingless Bee Propolis

Authors

Eriko Ishizu, Department of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 424-0414, Japan
Sari Honda, Department of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 424-0414, Japan
Tosihro Ohta, Department of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 424-0414, Japan
Boonyadist Vongsak, Faculty of Pharmaceutical Sciences, Burapha University, Chon Buri 22170, Thailand
Shigenori Kumazawa, Department of Food and Nutritional Sciences, University of Shizuoka, Shizuoka 424-0414, JapanFollow

Abstract

Propolis is a natural resin produced by honey bees from certain plants, has gained popularity as a food and alternative medicine. However, to the best of our knowledge, few studies on native Thailand stingless bee propolis are available. Information on the chemical composition and biological activities of propolis is needed to investigate its potential utility. Recently we have reported the possible plant origin of Thailand stingless bee propolis, Garcinia mangostana. In this study, further component analysis, functional evaluation, and identification of the plant origin of Thailand stingless bee propolis are conducted. Nine xanthones, including α-mangostin, garcinone C, γ-mangostin, cochinchinone T, β- mangostin, gartanin, 8-deoxygartanin, 9-hydroxycalabaxanthone, and mangostanol, were identified from the propolis. Comparative analysis of 70% ethanol extracts of Thailand stingless bee propolis (EEP) and the yellow resin from the fruit surface of G. mangostana (EEM) was performed using LC-MS, and similar chromatographic patterns were obtained. This result suggests that the plant origin of Thailand stingless bee propolis is confirmed to be the yellow resin from the fruit surface of G. mangostana. EEP and EEM were then tested for their ability to inhibit the tube formation of human umbilical vein endothelial cells, and both samples inhibited the tube formation of these cells in a concentrationdependent manner. This result indicates that Thailand stingless bee propolis may have future applications in the prevention and treatment of angiogenesis-related diseases.

Bahasa Abstract

Analisis Komponen dan Aktivitas Antiangiogenik dari Lebah Madu Tanpa Sengat Thailand. Propolis, campuran resin dari zat yang dikumpulkan oleh lebah madu dari tanaman tertentu, telah mendapatkan popularitas sebagai makanan dan obat alternatif. Namun, sejauh yang kami ketahui, hanya sedikit penelitian tentang propolis lebah madu tanpa sengat asli Thailand yang tersedia. Informasi tentang komposisi kimia dan aktivitas biologis propolis diperlukan untuk menyelidiki kegunaan potensialnya. Dalam penelitian ini, dilakukan analisis komponen, evaluasi fungsional, dan identifikasi asal tanaman dari propolis lebah madu tanpa sengat Thailand. Sembilan xanthone, termasuk α-mangostin, garcinone C, γ-mangostin, garcinone D, β-mangostin, gartanin, 8-deoxygartanin, 9-hydroxycalabaxanthone, dan mangostanol, diisolasi dari propolis. Analisis perbandingan dari 70% ekstrak etanol dari propolis lebah madu tanpa sengat Thailand (EEP) dan resin kuning dari permukaan buah Garcinia mangostana (EEM) dilakukan dengan menggunakan kromatografi cair fasa balik kinerja tinggi ditambah dengan spektrometri massa electrospray resolusi tinggi , dan pola-pola kromatografi serupa diperoleh. Hasil ini menunjukkan bahwa asal tanaman propolis lebah madu tanpa sengat Thailand adalah resin kuning dari permukaan buah G. mangostana. EEP dan EEM kemudian diuji kemampuannya untuk menghambat pembentukan tabung sel endotel vena umbilikal manusia, dan kedua sampel menghambat pembentukan tabung sel-sel ini dengan tergantung pada konsentrasi. Hasil ini menunjukkan bahwa propolis lebah madu tanpa seengat Thailand dapat memiliki aplikasi jangka panjang dalam pencegahan dan pengobatan penyakit terkait angiogenesis.

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Recommended Citation

Ishizu, Eriko; Honda, Sari; Ohta, Tosihro; Vongsak, Boonyadist; and Kumazawa, Shigenori (2019) "Component Analysis and Antiangiogenic Activity of Thailand Stingless Bee Propolis," Makara Journal of Technology: Vol. 23: Iss. 2, Article 5.
DOI: 10.7454/mst.v23i2.3703
Available at: https://scholarhub.ui.ac.id/mjt/vol23/iss2/5