Over consuming of caffeine is one of the factors to a few health problems such as insomnia, hypertension and cardiovascular disease. This preliminary study was conducted to evaluate the Caffeine-Imprinted Polymer (CAF-MIP) toxicity that was synthesized for a new alternative method for decaffeination. It is crucial to evaluate the toxicity of CAF-MIPas this product is potential to be used as complimentary with any drinks containing caffeine. In this study, the CAF-MIP toxicity potential was confirmed on Normal Chang Liver cell (NCLC) based on its IC50 value andacridine orange and propidium iodide (AO/PI) staining for mode of cell death observation.Proliferation assay was also conducted after 24, 48 and 72 hours at 30 μg/ml on NCLC and it showed that CAF-MIP promote NCLC growth as shown by at various concentrationof CAF-MIPincrease the percentage of NCLC viability. Observation under light microscopes on NCLC incubated wit CAF-MIP and NIP showed the normal, viable cell morphology, cuboidal and monolayer cell morphology and this can be seen with green fluorescence when view under fluorescence microscope. In conclusion, from this study, it is proved that the CAF-MIP does not initiate toxicity effects on human liver cells, meanwhile induction of cell proliferation was observed.

Bahasa Abstract

Evaluasi Ketoksikan Caffeine Imprinted Polymer (CAF-MIP) Secara In Vitro untuk Proses Dekafeinasi pada Sel Normal Hati Chang. Konsumsi kafein yang berlebihan merupakan salah satu faktor penyebab beberapa masalah kesehatan seperti insomnia, tekanan darah tinggi atau hipertensi dan penyakit kardiovaskular. Kajian ini bertujuan untuk menguji kadar ketoksiksan Caffeine Imprinted Polymer (CAF-MIP) yang telah disintesis untuk membantu proses dekafeinasi pada tubuh. Evaluasi ketoksikan CAF-MIP penting dilakukan, karena produk ini berpotensi untuk dikonsumsi bersama minuman yang mengandung kafein. Dalam kajian ini, potensi ketoksikan CAF-MIP telah dianalisis dengan menggunakan sel normal hati Chang (NCLC) berdasarkan nilai IC50 dan pewarnaan sel oleh akridinoren dan propidium iodide untuk menentukan nilai kematian sel. Analisis pergandaan sel juga telah dilakukan selama 24, 48, dan 72 jam pada konsentrasi 30 μg/mL terhadap NCLC. Hasil penelitian menunjukkan bahwa CAF-MIP menginduksi pertumbuhan NCLC yang bergantung pada konsentrasi CAF-MIP yang digunakan juga meningkatkan derajat viabilitas NCLC. Analisis di bawah mikroskop cahaya menunjukkan bahwa inkubasi NCLC bersama CAF-MIP dan NIP tidak mengubah bentuk sel seperti bentuk sel normal, morfologi sel yang viabel, berbentuk kuboid dan morfologi lapisan sel juga dapat dilihat dibawah mikroskopi floresen. Data penelitian ini telah menunjukkan CAF-MIP tidak menyebabkan ketoksikan pada sel-sel hati manusia, hasil ini juga menunjukkan bahwa sel tetap bisa bertambah.


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