Abstract
This paper discusses the results of improved optical probe that works according to optical fluorescence and absorption phenomena for measuring the phytoplankton suspension concentrations. Measurements are made on the Scenedesmus sp. culture. The laboratory test has shown that range of concentration from 104 up to around 1 x 106 cells/mL; fluorescence intensity at λ = 685 nm; and logarithmic of transmission intensity at λ = 405 nm are proportional to the cell concentration linear with proportional constant γ = 4 × 10-5 and β = –2 × 10-7 mL/cell respectively.
Bahasa Abstract
Penyempurnaan Probe Optik untuk Mengukur Konsentrasi Suspensi Phytoplankton dengan Memanfaatkan Fluoresensi dan Absorpsi Cahaya. Pada makalah ini dilaporkan hasil rancang bangun penyempurnaan probe optik untuk mengukur konsentrasi suspensi phytoplankton. Dari hasil pengujian skala laboratorium dengan menggunakan kultur Scenedesmus sp. ditunjukkan bahwa untuk rentang konsentrasi dari 104 hingga di sekitar 1 x 106 sel/mL intensitas fluoresensi pada λ = 685 nm memiliki hubungan yang linier terhadap konsentrasi sel dengan konstanta proporsianl γ = 4 × 10-5 mL/sel. Sementara itu untuk rentang konsentrasi yang sama, logaritma intensitas pada λ = 405 nm berbanding lurus dengan konsentrasi sel dengan konstatnta proporsional β = –2 × 10-7 mL/sel.
References
B.R. Vashishta, Botany for Degree Students: ALGAE, S. Chand, New Delhy, India, 1999, p.456.
L.E. Graham, W. Wilcox, Algae, Prentice Hall Inc., Upper Saddle River, USA, 2000, p.640.
E.J. D’Sa, S.E. Lohrenz, Applied Optic 38/12 (1999) 2524.
R.K.Y. Chan, K.M. Un, Applied Optic 40/12 (2001) 2956.
A.W. Herman, B. Beanlands, E.F. Philips, J. Plankton Res. 26 (2004) 1135.
J. Watson, S. Alexander, G. Craig, C.C. Hendry, P.R. Hobson, R.S. Lampitt, J.M. Marteau, H. Nareid, M.A. Palyer, K. Tipping, Meas. Sci. Technol. 12 (2001) L9.
E. Malkiel, O. Alquaddomi, J. Katz, Meas. Sci. Technol. 10 (1999) 1142
R.W. Novia, A. Handojo, H. Kurniawan, S. Sardy, Proceedings of Indonesia-Japan Joint Scientific Symposium, Chiba, Japan, 2004.
R. Wigajatri, A. Handojo, H. Kurniawan, N.B. Prihantini, Proceeding of The 8th International Conference on Quality in Research, Department of Electrical Engineering, University of Indonesia, Depok, Indonesia, 2005.
R. Wigajatri, N.B. Prihantini, A. Udhiarto, Prosiding Seminar Nasional Aplikasi Fotonika, Kampus ITS, Surabaya, 2008.
R.W.P. Novia, N.B. Prihantini, A. Udhiarto, Proceedings of Indonesia-Japan Joint Scientific Symposium, University of Chiba, Chiba, Japan, 2008.
R. Wigajatri, A. Handojo, H. Kurniawan, N.B. Prihantini, M.R.T. Siregar, Proceeding the International Conference on Opto-Electronics and Laser Application, Faculty of Engineering, University of Indonesia, Jakarta, Indonesia, 2002.
R. Wigajatri, N.B. Prihantini, A. Udhiarto, Instrumentasi 34/2 (2010) 137.
R. Wigajatri, N.B. Prihantini, RUUI Research Report, Universitas Indonesia, 2007.
W.D.P. Stewart, Algal Physiology and Biochemistry, Blackwell Scientific Publications
Ltd., California, 1974, p.346.
J.R.J.D. Ingle, S.R. Crouch, Spectrochemical Analysis, Prentice Hall, New Jersey, 1988, p.494.
R.W. Purnamaningsih, Doctor Desertation, Graduate Study Program on Opto-Electrotechnique and Laser Application, Faculty of Engineering, University of Indonesia, Jakarta, Indonesia, 2006.
Recommended Citation
Purnamaningsih, Retno Wigajatri and Prihantini, Nining Betawati
(2012)
"Improved Optical Probe for Measuring Phytoplankton Suspension Concentrations Based on Optical Fluoresensce and Absorption,"
Makara Journal of Technology: Vol. 16:
Iss.
2, Article 4.
DOI: 10.7454/mst.v16i2.1509
Available at:
https://scholarhub.ui.ac.id/mjt/vol16/iss2/4
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