Cassava pulp, a low cost solid byproduct of cassava starch industry, has been proposed as a high potential ethanolic fermentation substrate due to its high residual starch level, low ash content and small particle size of the lignocellulosic fibers. As the economic feasibility depends on complete degradation of the polysaccharides to fermentable glucose, the comparative hydrolytic potential of cassava pulp by six commercial enzymes were studied. Raw cassava pulp (12% w/v, particle size <320 >μm) hydrolyzed by both commercial pectinolytic (1) and amylolytic (2) enzymes cocktail, yielded 70.06% DE. Hydrothermal treatment of cassava pulp enhanced its susceptibility to enzymatic cleavageas compared to non-hydrothermal treatment raw cassava pulp. Hydrothermal pretreatment has shown that a glucoamylase (3) was the most effective enzyme for hydrolysis process of cassava pulp at temperature 65 °C or 95 °C for 10 min and yielded approximately 86.22% and 90.18% DE, respectively. Enzymatic pretreatment increased cassava pulp vulnerability to cellulase attacks. The optimum conditions for enzymatic pretreatment of 30% (w/v) cassava pulp by a potent cellulolytic/ hemicellulolytic enzyme (4) was achieves at 50 °C for 3, meanwhile for liquefaction and saccharification by a thermo-stable α-amylase (5) was achieved at 95 °C for 1 and a glucoamylase (3) at 50 °C for 24 hours, respectively, yielded a reducing sugar level up to 94,1% DE. The high yield of glucose indicates the potential use of enzymatic-hydrothermally treated cassava pulp as a cheap substrate for ethanol production.
H. Elkholy, A. Eltantawy, J. Root Crops 26 (2000) 1.
A. Akaracharanya, J. Kesornsit, N. Leepipatpiboon, T. Sri-norakutara, V. Kitpreechavanich, V. Tolieng, Ann. Microbiol. (2010.) DOI 10.1007/s13213-010-0155-8 Published online 15 November 2010.
I. Akpan, N. Uraih, C.O. Obuekwe, M.J. Ikenebomeh, Acta Biotechnol. 8 (2004) 39.
K. Sriroth, R. Chollakup, S. Chotineeranat, K. Piyachomkwan, G.C. Oates, Bioresour Technol 71
T. Srinorakutara, L. Kaewvimol, L. Saengow, J. Sci. Res. Chula. Univ. 31 (2006) 77.
H. Iwasaki, T. Kojima, Y. Matsumura, H. Yamamoto, K. Yamaji, K. Yamada, J. Jap. Inst. Ener. 86 (2007) 470 (in Japanese).
U. Rattanachomsri, S. Tanapongpipat, L.Eurwilaichitr, V. Champreda, J. Biosci Bioeng 107 (2009) 488.
A. Kosugi, A. Kondo, M. Ueda, Y. Murata, P. Vaithanomsat, W. Thanapase, T. Arai, Y. Mori, Renew. Energy 34 (2009) 1354.
J.S. Liu, W.X. Liu, C.L. Li, C.X. Shang, J.W. Yuan, Y.H. Wei, G.Z. Wang. K.R. Jiang, G.J. Yue, T.Y. Yu,WO 2010/03422-0 A1/1 April 2010.
AOAC, Official Methods of Analysis, 15th Ed. Association of Official Analytical Chemists, Washington, DC., 1990.
K. Sriroth, V. Santisopasri, C. Petchalanuwat, K. Kurotjanawong, K. Piyachomkwan, C.G. Oates, Carbohydr Polym. 38 (1999) 161.
S. Kajiwara, H. Maeda, Agric. Biol. Chem. 47 (1983) 2335.
L.D. Salvador, T. Suganuma, K. Kitahara, H. Tanoue, M. Ichiki, J. Agric. Food Chem. 48 (2000) 3448.
N. Thongchul, S. Navankasattusas, S.T. Yang, Bioprocess Biosyst Eng 33 (2010) 407.
L.D. Salvador, T. Suganuma, K. Kitahara, Y. Fukushige, H. Tanoue, J. Biosci Bioeng 93 (2002) 64.
L. Bailoni, S. Schiavon, G. Pagnin, F. Tagliapietra, M. Bonsembiante, Italy J. Anim. Sci. 4 (2005) 49.
D. Wang, S. Bean, J. McLaren, P. Seib, P. Madl, M. Tuinstra, J. Ind. Microbiol. Biotechnol. 35 (2008) 313.
S. Srikanta, S.A. Jaleel, N.P. Ghildyal, B.K. Lonsane, Die Nahrung. 36 (1992) 253.
S. Srichuwong, M. Fujiwara, X. Wang, T. Seyama, R. Shiroma, M. Arakane, N. Mukojima, K. Tokuyasu, Biomass Bioenergy 33 (2009) 890.
T. Godfrey, J. Reichelt, Industrial Enzymology, The Nature Press, New York. 1983, p.34.
M. Somogy, J. Biol. Chem. 195 (1952) 19.
J. Buchert, J.M. Koponen, M. Suutarinen, A. Mustranta, M. Lille, R. Tõrrõnen, K. Poutanen. J.
Sci. Food Agric.85 (2005) 2548.
Djumaáli, D. Primarini, W. Sumaryono, N. Sumarno, Soewarno, Prosiding Seminar Nasional Teknik Kimia UPN “Veteran” Surabaya (2010a) ISSN: 1978-0427.
A. Andersson, Y. Gekas, I. Lind, Y. Oliveira, R. Oste, Crit. Rev. in Food Sci. Nutr. 34 (1994) 229.
G.H. Robertson, D.W.S. Wong, C.C. Lee, K. Wagschal, M.R. Smith, W.J. Orts. J. Agric. Food Chem. 54 (2006) 353.
E.E. Perez, W.M. Brene, Y.A. Bahnassay, Starch/Starke 50 (1998) 70.
Y.H. Ahn, Y.L. Yang, C.Y. Choi, Biotech. Lett. 17 (1995) 547.
Djuma’ali, Djuma’ali; Soewarno, Nonot; Sumarno, Sumarno; Primarini, Dyah; and Sumaryono, Wahono
"Cassava Pulp as a Biofuel Feedstock of an Enzymatic Hydrolysis Proces,"
Makara Journal of Technology: Vol. 15:
2, Article 14.
Available at: https://scholarhub.ui.ac.id/mjt/vol15/iss2/14
Chemical Engineering Commons, Civil Engineering Commons, Computer Engineering Commons, Electrical and Electronics Commons, Metallurgy Commons, Ocean Engineering Commons, Structural Engineering Commons