Diafiltration by means of the ultrafiltration system of probiotic fermented Mung beans (Phaseolus radiatus L.) concentrate has been performed to reduce or eliminate salts and smaller impurities than the nominal cut-off of the membrane of 20,000 nominal weight cut-off (NWCO). These processes have been conducted as an attempt in order to get a probiotic product with organoleptic acceptability, composition, and the optimal total lactic acid bacteria (LAB) counts because the presence of salts will affect on the viability of LAB and the cell lysis of LAB and limit its utility in food products. Concentrate of probiotic mung beans was prepared through fermentation of LAB using inoculum of LAB consisting of Lactobacillus bulgaricus and Streptococcus thermophylus (1 : 1) on fermented mung beans extract inoculated by inoculum of Rhizopus–C1 in rice substrates at salt condition. Ultrafiltration and diafiltration modes have been carried out at flow rate of 8.77 Liter/minute, room temperature and the pressure of 5 bar (0 to 79.7 minutes) and 7 bar (0-154.5 minutes) with the ratio of the volume of pure water to the volume of initial feed (number of diavolume, Nd) of 0, 0.25, 0.5, 0.75, 1.0 and 1.25, respectively. The experiment results based on total LAB counts as a probiotic product show that a high Nd can reduce the salt content but increase the total LAB counts. Nd of 1.0 results reduce the salt content which is equal to retentate, permeate, and the optimal total LAB counts. Ultrafiltration and diafiltration modes at the pressure of 7 bar and Nd of 1.0 give a retentate with total solid of 6.1355%, salt of 1.3515% and remove 86.15% of the salt from probiotic fermented mung beans concentrate and total LAB counts of 10.73 log cycles. Meanwhile, the permeate obtained at this condition results in flux value of 10.83 Liter/m2.hour with contents of total solid of 6.8199%, salt of 1.325% and total LAB counts of 5.49 log cycles.


K.G. Lee, A.E. Mitchel, T. Shibamoto, J. Agric. Food Chem. 48 (2000) 4817.

A. Montano, A.H. Sanchez, A. De Castro, J. Food Sci. 65/6 (2000) 1022.

A. Simon, L. Vandanjon, G. Levesque, P. Bourseau, Desalin. 144 (2002) 314.

R.D. Noble, S.A. Stern, Membrane Separations Technology: Principles and Applications, Elsevier, Amsterdam, 1999, p. 394.

M. Cheryan, Ultrafiltration and Microfiltration Handbook, Technomic Publishing Co., Lancaster, 1997.

M. Cheryan, Ultrafiltration Handbook, Technomic Publishing Co., Lancaster, 1986, p. 206.

A.S. Grandison, M.J. Lewis, Separation Processes in the Food and Biotechnology Industries: Principles and Applications, Technomic Publishing Co. Inc., Lancaster, 1996, p. 95.

L. Wang, L. Song, J. Membr. Sci. 160 (1999) 41.

H.E. Shallo, A. Rao, A.P. Ericson, R.L. Thomas, Preparation of Soy Protein Concentrate by Ultrafiltration, J. Food Sci. 66/2 (2001) 242.

Salt Removal by Diafiltration [Brochure]. Ohio: Brochure of Amicon Corporation; 1981.

Operating Manual DSS Lab Unit M20, Denmark: DSS; 2000.

A.Y. Tamime, V.M.E. Marshall, Microbiology and Biochemistry of Cheese and Fermented Milk, Blackie Academic and Professional, London, 1997.



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