"Analysis of Differentially Expressed Genes (DEGS) Related to Interleuk" by Siva Fauziah, Weri Veranita et al.
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Indonesian Journal of Medical Chemistry and Bioinformatics

Abstract

Atopic eczema, also known as atopic dermatitis, is a chronic inflammatory skin condition characterized by itchy, red, and swollen skin. It is often associated with other atopic diseases such as asthma and hay fever. Interleukin-17 (IL-17), a pro-inflammatory cytokine, plays a crucial role in various inflammatory and autoimmune conditions, including atopic eczema. This study aims to identify potential therapeutic targets for managing atopic eczema based on the analysis of differentially expressed genes (DEGs). The expression of these gene targets was subsequently validated for their potential as biomarkers. Additionally, upstream regulator protein (URP) searches for the resulting DEGs were conducted. DEG analysis of the Gene Expression Omnibus (GEO) dataset, GSE6012 (atopic eczema vs. healthy donor skin), revealed that genes related to IL-17 signaling—FOSL1, MMP1, DEFB4B, S100A7, S100A8, and S100A9—can serve as biomarkers for atopic eczema with sensitivity and specificity values of 1.000. URP analysis suggested that inhibition of IL1A and NOG, as well as TGFB1 activity, are potential therapeutic targets to downregulate these six DEGs, thereby restoring their expression to the levels observed in healthy skin.

Bahasa Abstract

Eksim atopik, juga dikenal sebagai dermatitis atopik, adalah kondisi kulit inflamasi kronis yang ditandai dengan kulit gatal, merah, dan bengkak. Hal ini sering dikaitkan dengan penyakit atopik lainnya seperti asma dan demam. Interleukin-17 (IL-17), suatu sitokin pro-inflamasi, memainkan peran penting dalam berbagai kondisi inflamasi dan autoimun, termasuk eksim atopik. Penelitian ini bertujuan mengidentifikasi target terapi potensial untuk mengatasi eksim atopik berdasarkan analisis gen-gen yang diekspresikan secara berbeda (Differentially Expressed Genes, DEG). Target ekspresi gen tersebut kemudian divalidasi kemampuannya sebagai biomarker. Pencarian protein regulator hulu (URP) dari DEGs yang dihasilkan juga dilakukan. Hasil analisis DEG pada dataset GEO, yaitu GSE6012 (eksim atopik vs healthy donor skin) menunjukkan gen-gen terkait persinyalan IL-17: FOSL1, MMP1, DEFB4B, S100A7, S100A8, dan S100A9 dapat digunakan sebagai biomarker untuk eksim atopik dengan nilai sensitivitas dan spesifisitas 1.000. Analisis URP menunjukkan bahwa penghambatan IL1A dan NOG, serta aktivitas TGFB1 merupakan target terapi yang dapat digunakan untuk men-downregulasi ke-6 DEG tersebut agar ekspresinya kembali pada kondisi healthy skin.

References

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