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Indonesian Journal of Medical Chemistry and Bioinformatics

Abstract

Introduction: Breast cancer occurs quite high and is one of the main causes of death in Indonesia and the world. Current treatment for cancer is expensive and has varying degrees of success and can cause side effects for patients. Kluwak (Pangium edule), whose reseach is still limited has the potential to be used as an alternative treatment for breast cancer. Methods: Kluwak powder was macerated using three different types of solvents to obtain ethanol extract, ethyl acetate extract and n-hexane kluwak extract. Phytochemical tests and thin layer chromatography (TLC) were carried out to determine the type and amount of phytochemical components of kluwak extract. The antioxidant activity of kluwak extract was tested using the DPPH method and the cytotoxic effect on MCF-7 breast cancer cells was determined using the MTT test. Results: The phytochemical components contained in kluwak extract include flavonoids, alkaloids, triterpenoids and glycosides. The ethanol extract of kluwak showed very weak antioxidant activity (IC50 = 26459 µg/ml), while the antioxidant activity of the ethyl acetate and n-hexane extracts could not be determined. Ethyl acetate and n-hexane kluwak extracts also had a moderate cytotoxic effect on MCF-7 breast cancer cells with IC50 values respectively 132.79 µg/ml and 232.93 µg/ml while the ethanol extract had a weak cytotoxic effect with values IC50 667.91 µg/ml.Conclusion: Kluwak (Pangium edule) extract contains phytochemical compounds and cytotoxic effects on MCF-7 breast cancer cells, so it has the potential to be developed as a therapeutic agent in the management of breast cancer.

Bahasa Abstract

Latar belakang: Kanker payudara terjadi cukup tinggi dan merupakan salah satu penyebab utama kematian di Indonesia dan dunia. Pengobatan untuk kanker yang mahal dan memiliki tingkat keberhasilan yang berbeda-beda dan dapat menyebabkan efek samping kepada pasien sehingga diperlukan pengembangan terapi alternatif untuk pengobatan kanker payudara dengan tanaman herbal yang biayanya terjangkau dan memiliki efek samping minimal, salah satunya kluwak (Pangium edule). Metode: Bubuk kluwak dimaserasi dengan menggunakan tiga jenis pelarut berbeda sehingga diperoleh ekstrak etanol, ekstrak etil asetat, dan ekstrak n-heksana kluwak. Uji fitokimia dan kromatografi lapis tipis (KLT) dilakukan untuk mengetahui jenis dan jumlah komponen fitokimia ekstrak kluwak. Aktivitas antioksidan ekstrak kluwak dilakukan uji dengan metode DPPH dan efek sitotoksisitas terhadap sel kanker payudara MCF-7 diketahui melalui uji MTT. Hasil: Komponen fitokimia yang terkandung di dalam ekstrak kluwak mencakup flavonoid, alkaloid, triterpenoid, dan glikosida. Ekstrak etanol kluwak menunjukkan aktivitas antioksidan yang sangat lemah (IC50 = 26459 µg/ml), sedangkan ekstrak etil asetat dan n-heksana tidak dapat ditentukan aktivitas antioksidannya. Ekstrak etil asetat dan n-heksana kluwak juga memiliki efek sitotoksisitas yang sedang terhadap sel kanker payudara MCF-7 dengan nilai IC50berturut-turut 132,79 µg/ml dan 232,93 µg/ml sedangkan ekstrak etanol memiliki efek sitotoksisitas yang lemah dengan nilai IC50 667,91 µg/ml. Kesimpulan: Ekstrak kluwak (Pangium edule) memiliki kandungan senyawa fitokimia dan efek sitotoksisitas terhadap sel kanker payudara MCF-7 sehingga berpotensi untuk dikembangkan menjadi salah satu agen terapeutik dalam tatalaksana kanker payudara.

References

  1. International Agency for research on Cancer. Fact Sheet: Breast Cancer Globocan 2020 [Internet]. International Agency for research on Cancer: World Health Organization, 2020. Available from: https://gco.iarc.fr/today/data/factsheets/cancers/20-Breast-fact-sheet.pdf
  2. International Agency for research on Cancer. Fact Sheet: Indonesia Globocan 2020 [Internet]. International Agency for research on Cancer: World Health Organization, 2021. Available from: https://gco.iarc.fr/today/data/factsheets/populations/360-indonesia-fact-sheets.pdf
  3. Badan Penyelenggara Jaminan Sosial Kesehatan. Laporan pengelolaan program tahun 2020 dan laporan keuangan tahun 2020 (auditan) [Internet]. Badan Penyelenggara Jaminan Sosial Kesehatan: Indonesia, 2021. Available from: https://www.bpjs-kesehatan.go.id/bpjs/dmdocuments/b1ccf38a84e322f2cf5f648b857f1de0.pdf
  4. Trayes, K.P.; Cokenakes, S.E.H. Breast cancer treatment. Am Fam Physician, 2021, 104(2):171-178.
  5. Waks, A.G.; Winer, E.P. Breast cancer treatment: a review. JAMA, 2019, 321(3):288-300.
  6. El-Dahiyat, F.; Rashrash, M.; Abuhamdah, S.; Abu Farha, R.; Babar, Z.U. Herbal medicines: a cross-sectional study to evaluate the prevalence and predictors of use among Jordanian adults. J Pharm Policy Pract, 2020, 13:2.
  7. Wardani, R.K.; Putri, Y.M.; Nurjanah, D.A.; Susidarti, R.A. Mengungkap potensi kluwak (Pangium edule Reinw) sebagai antioksidan alami dan penghambat senescence. Jurnal Ilmu Kefarmasian Indonesia, 2021, 19(2): 189-96.
  8. Listyorini, K.I.; Kusumaningrum, H.D.; Lioe, H.N. Antifungal activity and major bioactive compounds of water extract of Pangium edule seed against Aspergillus flavus. Int J Food Sci, 202.
  9. Budianto, R.; Ali, H.M.; Abustam, E. Antioxidant activites of beef sausage with addition of Kluwak (Pangium edule Reinw). J Anim Sci, 2019, 1(1): 45-54.
  10. Warnasih, S.; Hasanah, U. Phytochemical characterization and tannin stability test from kluwek (Pangium edule Reinw). Journal of Science Innovare, 2018, 1(2): 44-49.
  11. Zamaniyah, M. Perbandingan aktivitas sitotoksik fraksi n-heksan ekstrak etanol daging biji kluwak (Pangium edule R.) dengan metode ekstraksi maserasi dan ultrasonic terhadap sel kanker payudara T47D [Skripsi]. Semarang: Fakultas Farmasi Universitas Wahid Hasyim Semarang, 2018.
  12. Prasetyoy, E.H. Uji aktivitas sitotoksik fraksi n-heksan ekstrak etanol daging biji kluwak (Pangium edule R.) dengan metode ultrasonic terhadap sel kanker serviks HELA [Skripsi]. Semarang: Fakultas Farmasi Universitas Wahid Hasyim Semarang, 2018.
  13. Pangisian, J.; Sangi, M.S.; Kumaunang, M. Analisis senyawa metabolit sekunder dan uji aktivitas antiosidan serta antibakteri biji buah pangi (pangium edule reinw). Jurnal LPPM Bidang Sains dan Teknologi, 2022, 7(1): 11-19.
  14. Warnasih, S.; Hasanah, U. Ekstraksi zat warna dari kluwek (Pangium edule Reinw.) menggunakan berbagai pelarut. Ekologia. 2018, 1(1): 40-48.
  15. Zhang, Y.; Cai, P.; Cheng, G.; Zhang, Y. A brief review of phenolic compounds identified from plants: Their extraction, analysis, and biological activity. Natural Product Communications, 2022, 17(1): 1934578X211069721.
  16. Chiocchio, I.; Mandrone, M.; Tomasi, P.; Marincich, L.; Poli, F. Plant secondary metabolites: An opportunity for circular economy. Molecule, 2021, 26(2): 495.
  17. Karak, P. Biological activities of flavonoids: an overview. Int. J. Pharm. Sci. Res, 2019, 10(4): 1567-74.
  18. Ghosh, S. Triterpenoids: Structural diversity, biosynthetic pathway, and bioactivity. Studies in Natural Products Chemistry, 2020, 67: 411-61.
  19. Tong, W. Biotransformation of terpenoids and steroids. Natural Products, Springer, 2018: 2733-59.
  20. Bribi, N. Pharmacological activity of alkaloids: a review. Asian Journal of Botany, 2018, 1(1):1-6.
  21. Khan, H.; Saeedi, M.; Nabavi, S.M.; Mubarak, M.S.; Bishayee, A. Glycosides from medicinal plants as potential anticancer agents: emerging trends towards future drugs. Curr Med Chem, 2019, 26(13): 2389-2406.
  22. Baliyan, S.; Mukherjee, R.; Priyadarshini, A.; Vibhuti, A.; Gupta, A.; Pandey, R.P.; Chang, C.M. Determination of antioxidants by dpph radical scavenging activity and quantitative phytochemical analysis of ficus religiosa. Molecules, 2022, 27(4): 1326.
  23. Widyantari, N.P.; Sari, P.M. Review: Aktivitas antioksidan ekstrak herba suruhan. Jurnal Farmasi dan Kesehatan Indonesia. 2023, 3(1): 1-3.
  24. Warasari, R.T.P. Aktivitas antioksidan pada ekstrak kasar kluwek (Pangium edule Reinw) dengan metode microwave assisted extraction (kajian rasio bahan:pelarut dan waktu ekstraksi) [Skripsi]. Malang: Fakultas Teknologi Pertanian Universitas Brawijaya, 2018.
  25. Riss, T.; Niles, A.; Moravec, R.; et al. Cytotoxicity Assays: In Vitro Methods to Measure Dead Cells, 2019.
  26. Saroyo, H.; Saputri, N.F. Cytotoxicity of mangrove leaves (rhizophora) ethanolic extract on cancer cells. Journal of Nutraceuticals and Herbal Medicine, 2021, 4(1):43-52.
  27. Sudhakaran, M.; Sardesai, S.; Doseff, A.I. Flavonoids: New frontier for immuno-regulation and breast cancer control. Antioxidants (Basel), 2019, 8(4): 103.

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