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Indonesian Journal of Medical Chemistry and Bioinformatics

Abstract

Cervical cancer is the fourth highest cancer occurring and causes death in women. Therefore, adequate management is needed to prevent its development. Currently, the treatment has a variety of adverse side effects, so it needs alternative treatments that are supportive and with minimal side effects. One way is to use herbal plants, such as the Kunto Dewo (Kigelia pinnata) plant which is often used as traditional medicine. This plant has antimicrobial and cytotoxic effects on cancer cells. Knowing the phytochemical profile and in-vitro anticancer activity of the n-hexane extract of peel and flesh of Kunto Dewo (Kigelia pinnata) fruit against cervical cancer HeLa cells. The peel and flesh of Kigelia pinnata fruit are macerated in n-hexane solvent then the resulting filtrate is evaporated to become an extract. The extract is used for phytochemical profile, carry out through phytochemical screening, thin layer chromatography, calculation of total phenol, and total flavonoids. The extract was also tested for cytotoxic activity against cervical cancer HeLa cells using MTT assay. The n-hexane extract of the peel and flesh of the kigelia pinnta fruit contains triterpenoids. In TLC analysis, there were found 4 components in the n-hexane extract of Kigelia pinnata fruit peel and 8 components in the n-hexane extract of Kigelia pinnata fruit flesh. The cytotoxic activity of the n-hexane extract of the peel and flesh of Kigelia pinnata fruit is included in the moderately active category. The n-hexane extract of the peel and flesh of the kigelia pinnata fruit has potential as an anti-cervical cancer.

Bahasa Abstract

Kanker serviks merupakan kanker tertinggi keempat yang terjadi dan menyebabkan kematian pada wanita. Oleh karena itu, diperlukan pengelolaan yang memadai untuk mencegah perkembangannya. Saat ini pengobatan tersebut memiliki berbagai efek samping yang merugikan, sehingga diperlukan pengobatan alternatif yang bersifat suportif dan dengan efek samping yang minimal. Salah satu caranya adalah dengan memanfaatkan tanaman herbal, seperti tanaman Kunto Dewo (Kigelia pinnata) yang sering digunakan sebagai obat tradisional. Tanaman ini memiliki efek antimikroba dan sitotoksik pada sel kanker. Mengetahui profil fitokimia dan aktivitas antikanker in-vitro ekstrak n-heksan kulit dan daging buah Kunto Dewo (Kigelia pinnata) terhadap sel HeLa kanker serviks. Kulit dan daging buah Kigelia pinnata dimaserasi dalam pelarut n-heksan kemudian filtrat yang dihasilkan diuapkan menjadi ekstrak. Ekstrak tersebut digunakan untuk profil fitokimia, dilakukan melalui skrining fitokimia, kromatografi lapis tipis, perhitungan fenol total, dan flavonoid total. Ekstrak juga diuji aktivitas sitotoksiknya terhadap sel kanker serviks HeLa menggunakan uji MTT. Ekstrak n-heksana kulit dan daging buah kigelia pinnta mengandung triterpenoid. Pada analisis KLT ditemukan 4 komponen pada ekstrak n-heksana kulit buah Kigelia pinnata dan 8 komponen pada ekstrak n-heksana daging buah Kigelia pinnata. Aktivitas sitotoksik ekstrak n-heksan kulit dan daging buah Kigelia pinnata termasuk dalam kategori cukup aktif. Ekstrak n-heksana kulit dan daging buah kigelia pinnata berpotensi sebagai anti kanker serviks.

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Srisawat T, Chumkaew P, Heed-chim W, Sukpondma Y, Kanokwiroon K. Phytochemical screening and cytotoxicity of Crude extracts of vatica diospyroides symington type LS. Trop J Pharm Res, February 2013;12 (1):71-6

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