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Indonesian Journal of Medical Chemistry and Bioinformatics

Abstract

Abstract

Introduction: Type 2 Diabetes Mellitus (DM) is a metabolic disorder characterized by hyperglycemia caused by B cell dysfunction, insulin resistance, or both. Indonesia and worldwide have an increasing incidence that leads to a significant socio-economic burden. The effective treatment of Type 2 DM with minimal side effects is still challenging. This research aims to identify phytochemical properties in ethanol extract of Mangifera quadrifida and the effectivity as an alfa-glucosidase inhibitor beneficial to research on the treatment of type 2 DM.

Method: Mangifera quadrifida was extracted by ethanol as solvent. The phytochemical analysis is conducted by phytochemical screening methods and thin-layer chromatography (TLC). The antidiabetic properties in ethanol extract of Mangifera quadrifida is tested by inhibitory activity on alpha-glukosidase.

Result: The ethanol extract of Mangifera quadrifida contains tanin, triterpenoid, flavonoid, and glycoside. TLC test on the ethanol extract of Mangifera quadrifida was detected 4 points (Rf 0,15; 0,464, 0,511; and 0,63). The IC50 to α-glucosidase of Mangifera quadrifida and the acarbose as positive control are 18,19 ppm and 4,88 ppm respectively.

Discussion: Tanin, triterpenoid, flavonoid, and glycoside of Mangifera quadrifida have antidiabetic properties. The result on IC50 value in ethanol extract of Mangifera quadrifida is among the active class of antidiabetic group although the value is higher the acarbose.

Conclusion: Ethanol extract of the Mangifera quadrifida has phytochemical compunds that have antiadiabetic potential. Acarbose has better IC50 to α-glucosidase compared to Ethanol extract of Mangifera quadrifida.

Bahasa Abstract

Abstrak

Pendahuluan : Diabetes Mellitus (DM) Tipe 2 merupakan penyakit metabolik yang ditandai dengan hiperglikemia yang disebabkan karena disfungsi sel B, resistensi insulin, atau keduanya. Indonesia dan dunia mengalami peningkatan kejadian sehingga mengarah pada beban sosio-ekonomi yang cukup signifikan. Pengobatan yang efektif bagi DM tipe 2 dengan efek samping rendah masih menjadi tantangan. Penelitian ini bertujuan untuk mengidentifikasi sifat fitokimia pada ekstrak etanol dari Mangifera quadrifida dan efektivitasnya sebagai penghambat alfa-glukosidasi yang bermanfaat pada penelitian pengobatan DM tipe 2.

Metode : Mangifera quadrifida diekstrak menggunakan etanol sebagai pelarut. Analisis fitokimia dilakukan dengan metode skrining fitokimia dan Kromatografi Lapis Tipis (KLT). Sifat antidiabetik pada ekstrak etanol Mangifera quadrifida diuji menggunakan aktivitas inhibisi pada alfa-glukosidase.

Hasil : Ekstrak etanol Mangifera quadrifida mengandung tanin, triterpenoid, flavonoid, dan glikosida. Uji KLT pada ekstrak etanol Mangifera quadrifida menghasilkan 4 titik (Rf 0,15; 4,464; 0,511; dan 0,63). IC50 dari alfa-glukosidase pada Mangifera quadrifida dan acarbose sebagai kontrol positif masing-masing adalah 18,19 ppm dan 4,88 ppm.

Pembahasan : Tanin, triterpenoid, flavonoid, dan glikosida dari Mangifera quadrifida memiliki sifat antidiabetik. Hasil pada nilai IC50 ekstrak etanol Mangifera quadrifida tergolong pada active class dari grup antidiabetik meskipun nilainya lebih tinggi dari acarbose.

Simpulan : Ekstrak etanol dari Mangifera qudrifida memiliki senyawa fitokimia yang berpotensi sebagai antidiabetik. Acarbose memiliki IC50 lebih baik untuk alfa-glukosidase dibandingkan dengan ekstrak etanol dari Mangifera quadrifida.

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