Tracing Antibacterial Compounds from Kaledang (Artocarpus lanceifolius Roxb.) Stem Bark

Authors

Hamsidar Hasan, Department of Pharmacy, Faculty of Sports and Health, Gorontalo State University, IndonesiaFollow
Tety Tuloli, Department of Pharmacy, Faculty of Sports and Health, Gorontalo State University, IndonesiaFollow
Syamsul Bahri, Department of Animal Husbandry, Faculty of Agriculture, Gorontalo State University, IndonesiaFollow
La Ode Aman, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Gorontalo State University, IndonesiaFollow

Abstract

Artocarpus lanceifolius Roxb. or kaledang is a species of the mulberry family, Moraceae. Several extracts from its family have been reported to have antibacterial activity. Thus, it is necessary to determine compounds that have nature as antibacterial to be raw materials for antibacterial herbal medicines. The purpose of the study was to trace compounds that have antibacterial activity from Artocarpus lanceifolius stem bark. The study method was extraction by graded maceration, fractionation with column vacuum chromatography (CVC), isolation by radial chromatography, determination of antibacterial activity by agar diffusion method using the Gram positive and Gram negative bacteria. The characterization result of antibacterial compounds from Artocarpus lanceifolius chloroform extract stem bark showed the presence of prenylated flavonoid compound, namely 14-hydroxyartonin E (previously reported) with a diameter of inhibition zone at 5% concentration was 14.5 ± 0.1 mm against S. aureus (medium category), and 16.20 ± 0.2 mm against S. mutans (strong category), 14.3 ± 0.2 mm against E. coli (medium category), and 16.8± 0.7 mm against P. aeruginosa (strong category). It is concluded that 14-hydroxyartonin E in Artocarpus lanceifolius showed potential antibacterial activity.

References

Araya-Cloutier C., Vincken, J.P., Van De Schans, M.G.M., Hageman, J., Schaftenaar, G., Den Besten, H.M.W., et al. (2018). QSAR-based molecular signatures of prenylated (iso) flavonoids underlying antimicrobial potency against and membrane-disruption in gram positive and gram negative bacteria. Scientific Reports, 8(1):1–14.

Cao, S., Butler, M.S., Buss, A.D. (2003). Flavonoids from Artocarpus lanceifolius. Natural Product Research, 17(2):79–81.

Gurning, K., Siahaan, D., Iksen, I. (2020).Antibacterial activity test of extract ethanol of jackfruit leaves (Artocarpus heterophyllus. Lamk.) of bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis and Salmonella typhi. Journal of Pharmaceutical Sciences, 2(2):49–54.

Greenwood, D., 1995. Antimicrobial treatments. Sixty year of antimikrobial drug resistance comes of age. Lancet 346. Suppl.p. s 1

Hatano, T., Shintani, Y., Aga, Y., Shiota, S., Tsuchiya, T., Yoshida, T. (2000). Phenolic constituents of licorice. VIII. Structures of glicophenone and glicoisoflavanone, and effects of licorice phenolics on methicillin-resistant Staphylococcus aureus. Chemical and Pharmaceutical Bulletin, 48(9):1286–92.

Hashim, N.M., Rahmani, M., Ee, G.C.L., Sukari, M.A., Yahayu, M., Amin, M.A.M., Ali, A.M., Go, R. (2012). Antioxidant, antimicrobial, and tyrosinase inhibitory activities of xanthones isolated fom Artocarpus obtusus F.M.Jarret. Molecules, 17, 6071-6082

Heyne. (1987). Tumbuhan Berguna Indonesia. Jilid III. Cetakanke 1. Jakarta: Badan litbang kehutanan Jakarta. Departemen Kehutanan. Gatot subroto. 1374-1380.

Hayun, Arrahman, A., Suryadi, H., Yanuar, A. 2014. Uji Aktivitas antibakteri 1-[(kuinazolin-4-on-2-il)metil] piridin-1-ium-bromida dan 2-bromometilkuinazolin-4- on. Pharmaceutical Sciences and Research, 1(1), 1-8.

Jamil, S., Mariam, S., Awanis, S., Jemaon, N., Sirat, H.M., 2014. Antimikrobial flavonoid from Artocarpus anisophyllus Miq. and Artocarpus lowii King. Jurnal Teknologi, 71:1. Pp 95-99

Mawea, F., Maarisit, W., Datu, O., Potalangi, N. (2019). Efektivitas ekstrak daun cempedak Artocarpus integer sebagai antibakteri. The Tropical Journal of Biopharmaceutical, 2(1):115–22

Markham, K. (1988). Cara Mengidentifikasi Flavonoid, terjemahan Kosasih Padmawinata. Bandung: Penerbit ITB; 1988.

Osorio, M., carvajal, M., Vergara, A., Butassi, E., Zacchino, S., Mascayano, C., Montoya, M., Mejias, S., Cortez, M., Martinez, V. (2021). Prenylated flavonids with potential antimicrobial activity synthesis biological activity and in silico study. International Journal of Molecular Sciences. Doi: 10.33399/ijms22115472

Nomura, T., Hano, Y., Aida M. (1998). Isoprenoid- substituted flavonoids from artocarpus plants (Moraceae). ChemInform, 29(26).

Prastiyanto, M.E. (2021). Seeds extract of three artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (mdr) Escherichia coli isolates from urinary tract infections (utis). Biodiversitas, 22(10):4356–62.

Setiawan, R., Hamzah, H., Wirnawati. 2022. The efficacy of secondary metabolit compound in kaledang plant (Artocarpus lanceifoliu Roxb) as anticytoxic: Literatur review. International Journal of Medical Sciense and Dental Research, Vol 5 Issue 01. PP 44-49.

Suhartati, T., Wulandari, G.S., Yandri., Hadi, S. (2019). The antibacterial and anticancer test of cyclomulberochromen compounds from Artocarpus altilis. Research Journal of Chemistry and Environment, 23(9):10–6.

Syah, Y.M. (2016). Dasar-dasar penentuan struktur senyawa alam: Senyawa aromatik. Laboratorium Spektroskopi Massa dan NMR Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Teknologi Bandung; 2016.

Syafitri, I.F., Ersam, T. (2016). Senyawa sikloartobiloksanton dari kulit akar. Jurnal Sains dan Seni ITS, 5(2):80–4.

Ventola, 2015. The antibiotic resistance Crisis. PubMed Central, Vol.40(4) PMC 4378521

WHO. (2014).Global Report on Surveillance 2014. WHO 2014 AMR Rep [Internet].1–8. Available from: http://www.who.int/drugresistance/documents/AMR_ report_Web_slide_set.pdf.

Xie, Y.,Yang, W.,Tang, F., Chen, X., Ren, L. (2014). Antibacterial activities of flavonoids: Structure-activity relationship and mechanism. Current Medicinal Chemistry, 22(1):132–49.

Yang, X., Jiang, Y., He, J., Sun, J., Chen, F., Zhang, M., Yang, B., (2015). Prenylated flavonoids, Promising nutraceuticals with impressive biological activities. Trend in Food Science and technology, 44(1), 93-104.

Zakaria, Z., Soekamto, N.H., Syah, Y.M., Firdaus, F. (2017).Aktivitas antibakteri dari fraksi Artocarpus integer (thunb.) merr. dengan metode difusi agar (Antibacterial activity of Artocarpus integer (thunb.) Merr. fraction by difusi agar method). Jurnal Agro Industri Perkebunan, 12(2):1.

Recommended Citation

Hasan, Hamsidar; Tuloli, Tety; Bahri, Syamsul; and Aman, La Ode (2023) "Tracing Antibacterial Compounds from Kaledang (Artocarpus lanceifolius Roxb.) Stem Bark," Pharmaceutical Sciences and Research: Vol. 10: No. 3, Article 8.
DOI: 10.7454/psr.v10i3.1275
Available at: https://scholarhub.ui.ac.id/psr/vol10/iss3/8