Ethanolic extract of bacang mango leaves (Mangifera foetida L.) decreased iron concentration in blood Sprague dawley rats that had been induced iron overload. The aim of this experiment was to determine acute toxicity (LD50) value of ethanolic extract of M.foetida by weil method and its effect to blood component. This study was conducted by employing a complete random design using 25 male and 25 female mice of DDY strain which devided into 5 groups. The first group until fourth group were administered ethanolic extract of M.foetida with dose variation which suspension in aquadest orally. The fifth group was control that administered aquadest. The LD50 was determined by the total of death on all group. LD50 value of the extract showed no death in the biggest doses (13.013 g/kg). The examination was continued with measured blood count (erythrocites, trombocytes, leukocytes, and haemoglobin concentration). It was shown that the extract at dose 1.626; 3.253; 6.506 and 13.013 g/kg unchanged the blood count measurement.

Bahasa Abstract

Ekstrak etanol daun mangga bacang (Mangifera foetida L.) dapat menurunkan konsentrasi besi dalam darah tikus galur Sprague dawley yang telah diinduksi dengan besi berlebih (iron overload). Tujuan penelitian ini adalah menetukan nilai toksisitas akut (LD50) dari ekstrak etanol M.foetida dengan menggunakan metode weil dan pengaruhnya terhadap komponen dalam darah. Penelitian ini menggunakan desain randomisasi secara acak. Pada penelitian ini menggunakan 25 mencit jantan dan 25 mencit betina galur DDY yang dibagi menjadi 5 kelompok. Kelompok pertama sampai keempat merupakan kelompok yang diberikan ekstrak etanol M.foetida dengan dosis bertingkat yang disuspensikan dengan aquades yang diberikan secara oral. Kelompok kelima merupakan kelompok kontrol yang diberikan aquades. Nilai LD50 ditentukan dengan cara menghitung jumlah kematian total dari semua kelompok. Hasil LD50 dari ekstrak menunjukkan tidak adanya kematian sampai pada dosis terbesar (13,013 g/kg). Pengukuran dilanjutkan dengan penghitungan komponen darah (eritrosit, trombosit, leukosit, dan konsentrasi hemoglobin). Hasil menunjukkan bahwa ekstrak pada dosis 1,626; 3,253; 6,506 and 13,013 g/kg tidak mengubah dari jumlah atau konsentrasi komponen darah.


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